Genetic diversity of HIV type 1 in rural eastern Cameroon
To monitor the presence of genotypic HIV-1 variants circulating in eastern Cameroon, blood samples from 57 HIV-1-infected individuals attending 3 local health centers in the bordering rural villages with Central African Republic (CAR) were collected and analyzed phylogenetically. Out of the 40 HIV-1...
Saved in:
Published in | Journal of acquired immune deficiency syndromes (1999) Vol. 37; no. 5; p. 1641 |
---|---|
Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
15.12.2004
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | To monitor the presence of genotypic HIV-1 variants circulating in eastern Cameroon, blood samples from 57 HIV-1-infected individuals attending 3 local health centers in the bordering rural villages with Central African Republic (CAR) were collected and analyzed phylogenetically. Out of the 40 HIV-1 strains with positive polymerase chain reaction (PCR) profile for both gag and env-C2V3,12 (30.0%) had discordant subtype or CRF designation: 2 subtype B/A (gag/env), 1 B/CRF01, 2 B/CRF02, 1 CRF01/CRF01.A, 2 CRF11/CRF01, 1 CRF13/A, 1 CRF13/CRF01, 1 CRF13/CRF11, and 1 G/U (unclassified). Twenty-eight strains (70.0%) had concordant subtypes or CRF designation between gag and env: 27 subtype A and 1 F2. Out of the remaining 17HIV-1 strains negative for PCR with the env-C2V3 primers used, 10 (58.8%) had discordant subtype or CRF, and 7 (41.2%) had concordant one based on gag/pol/env-gp41 analysis. Altogether, a high proportion (22/57, 38.6%) of the isolates were found to be recombinant strains. In addition, an emergence of new forms of HIV-1 strains, such as subtype B/A (gag/env), B/CRF01 and B/CRF02, was identified. The epidemiologic pattern of HIV-1 in eastern Cameroon, relatively low and high prevalence of CRF02 and CRF11, respectively, was more closely related to those of CAR and Chad than that of other regions of Cameroon, where CRF02 is the most predominant HIV-1 strain. These findings strongly suggest that this part of Cameroon is a potential hotspot of HIV-1 recombination, with a likelihood of an active generation of new forms of HIV-1 variants, though epidemiologic significance of new HIV-1 forms is unknown. |
---|---|
AbstractList | To monitor the presence of genotypic HIV-1 variants circulating in eastern Cameroon, blood samples from 57 HIV-1-infected individuals attending 3 local health centers in the bordering rural villages with Central African Republic (CAR) were collected and analyzed phylogenetically. Out of the 40 HIV-1 strains with positive polymerase chain reaction (PCR) profile for both gag and env-C2V3,12 (30.0%) had discordant subtype or CRF designation: 2 subtype B/A (gag/env), 1 B/CRF01, 2 B/CRF02, 1 CRF01/CRF01.A, 2 CRF11/CRF01, 1 CRF13/A, 1 CRF13/CRF01, 1 CRF13/CRF11, and 1 G/U (unclassified). Twenty-eight strains (70.0%) had concordant subtypes or CRF designation between gag and env: 27 subtype A and 1 F2. Out of the remaining 17HIV-1 strains negative for PCR with the env-C2V3 primers used, 10 (58.8%) had discordant subtype or CRF, and 7 (41.2%) had concordant one based on gag/pol/env-gp41 analysis. Altogether, a high proportion (22/57, 38.6%) of the isolates were found to be recombinant strains. In addition, an emergence of new forms of HIV-1 strains, such as subtype B/A (gag/env), B/CRF01 and B/CRF02, was identified. The epidemiologic pattern of HIV-1 in eastern Cameroon, relatively low and high prevalence of CRF02 and CRF11, respectively, was more closely related to those of CAR and Chad than that of other regions of Cameroon, where CRF02 is the most predominant HIV-1 strain. These findings strongly suggest that this part of Cameroon is a potential hotspot of HIV-1 recombination, with a likelihood of an active generation of new forms of HIV-1 variants, though epidemiologic significance of new HIV-1 forms is unknown. |
Author | Zekeng, Leopold Takemura, Taichiro Ndembi, Nicaise Kobayashi, Eiji Ngansop, Charlotte Kaptue, Lazare Ichimura, Hiroshi Hayami, Masanori Takehisa, Jun Kageyama, Seiji Ido, Eiji Songok, Elijah M |
Author_xml | – sequence: 1 givenname: Nicaise surname: Ndembi fullname: Ndembi, Nicaise organization: Department of Viral Infection and International Health, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan – sequence: 2 givenname: Jun surname: Takehisa fullname: Takehisa, Jun – sequence: 3 givenname: Leopold surname: Zekeng fullname: Zekeng, Leopold – sequence: 4 givenname: Eiji surname: Kobayashi fullname: Kobayashi, Eiji – sequence: 5 givenname: Charlotte surname: Ngansop fullname: Ngansop, Charlotte – sequence: 6 givenname: Elijah M surname: Songok fullname: Songok, Elijah M – sequence: 7 givenname: Seiji surname: Kageyama fullname: Kageyama, Seiji – sequence: 8 givenname: Taichiro surname: Takemura fullname: Takemura, Taichiro – sequence: 9 givenname: Eiji surname: Ido fullname: Ido, Eiji – sequence: 10 givenname: Masanori surname: Hayami fullname: Hayami, Masanori – sequence: 11 givenname: Lazare surname: Kaptue fullname: Kaptue, Lazare – sequence: 12 givenname: Hiroshi surname: Ichimura fullname: Ichimura, Hiroshi |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15577423$$D View this record in MEDLINE/PubMed |
BookMark | eNo1j81KxDAURrMYcX70FSQvEL03uUmTpRSdGRhwo26HNE2hMk1L2hH69hbU1YGzOHzflq1SnyJjHOERwRVPACiNUiQkAKFEDQIW51Zsg1pqQaj0mm3H8WuxhsjdsjVqXRQk1Ya5fUxxagOv2--Yx3aaed_ww_GTT_MQOfI28XzN_sKjH6eYEy99F3Pfpzt20_jLGO__uGMfry_v5UGc3vbH8vkkgjbSCWWXSRqtLIJRQKAtBFIqkEfrqyCrBmqytgJPRgYsCkMuoPHBB5LaWrljD7_d4Vp1sT4Pue18ns__H-QPJiRHSg |
CitedBy_id | crossref_primary_10_1177_0956462414556328 crossref_primary_10_1089_aid_2008_0124 crossref_primary_10_1089_aid_2008_0223 crossref_primary_10_2174_1874613600903010014 crossref_primary_10_1089_aid_2006_22_1036 crossref_primary_10_1097_01_qai_0000219784_81163_2e crossref_primary_10_1002_jmv_20912 crossref_primary_10_1089_aid_2012_0182 crossref_primary_10_1186_1471_2377_10_60 crossref_primary_10_1177_2325957413510607 crossref_primary_10_1007_s15010_013_0568_y crossref_primary_10_1097_QAI_0b013e318269c414 crossref_primary_10_1371_journal_pone_0225575 crossref_primary_10_1089_aid_2006_0177 crossref_primary_10_1097_QAD_0b013e328045c4bd crossref_primary_10_1089_aid_2008_0018 crossref_primary_10_1097_01_COH_0000232344_23533_be crossref_primary_10_1097_01_qai_0000223017_01568_e7 crossref_primary_10_1097_01_qai_0000226793_16216_55 crossref_primary_10_1016_j_jviromet_2009_01_004 crossref_primary_10_1128_CVI_00483_10 crossref_primary_10_1016_j_jcv_2011_06_014 crossref_primary_10_1016_S1473_3099_10_70186_9 crossref_primary_10_1128_JCM_00428_07 crossref_primary_10_1016_j_jcv_2010_04_008 crossref_primary_10_3390_ijerph5050378 crossref_primary_10_1128_JVI_00889_07 crossref_primary_10_1016_j_meegid_2005_12_001 crossref_primary_10_1007_s00705_015_2623_8 crossref_primary_10_1089_aid_2006_22_936 crossref_primary_10_1089_aid_2009_0229 crossref_primary_10_1089_aid_2006_22_812 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1097/00126334-200412150-00019 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine |
ExternalDocumentID | 15577423 |
Genre | Research Support, Non-U.S. Gov't Journal Article Comparative Study |
GeographicLocations | Chad Cameroon Central African Republic |
GeographicLocations_xml | – name: Cameroon – name: Chad – name: Central African Republic |
GroupedDBID | --- .-D .GJ .XZ .Z2 01R 0R~ 1J1 354 40H 4Q1 4Q2 4Q3 53G 5GY 5RE 5VS 77Y 7O~ 8L- AAAAV AAAXR AAGIX AAHPQ AAIQE AAJCS AAMOA AARTV AASOK AAXQO ABBUW ABDIG ABIVO ABJNI ABXVJ ABZAD ACCJW ACDDN ACEWG ACGFO ACGFS ACILI ACOAL ACPRK ACWDW ACWRI ACXNZ ADGGA ADHPY ADNKB AE3 AE6 AEETU AFDTB AFEXH AFRAH AFUWQ AGINI AHMBA AHOMT AHQNM AHVBC AIJEX AINUH AJIOK AJNWD AJNYG AJZMW ALMA_UNASSIGNED_HOLDINGS AMJPA AMNEI AWKKM BKOMP BOYCO BQLVK BYPQX C45 CGR CS3 CUY CVF DIWNM DUNZO E.X ECM EIF EX3 F2K F2L F2M F2N F5P FCALG FL- FRJ FRP FW0 H0~ HZ~ IKREB IKYAY IN~ JF9 JG8 JK3 JK8 K8S KD2 KMI L-C NPM N~7 N~B O9- OAG OAH OCUKA ODA ODMTH OHH OHYEH OLG OLH OLU OLV OLW OLY OLZ OPUJH ORVUJ OUVQU OVD OVDNE OVIDH OVLEI OWU OWV OWX OWY OWZ OXXIT P2P PQQKQ RIG RLZ RXW S4R S4S T8P TAE TEORI V2I VVN W2D W3M WOQ WOW X3V X3W XJT XXN XYM YHZ YOC ZGI ZZMQN |
ID | FETCH-LOGICAL-c5629-3821551827c63040580c433c4a18abc2bf0d488b0a462c177649c16acac425882 |
ISSN | 1525-4135 |
IngestDate | Sat Sep 28 08:37:27 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 5 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c5629-3821551827c63040580c433c4a18abc2bf0d488b0a462c177649c16acac425882 |
OpenAccessLink | https://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=38634&item_no=1&attribute_id=26&file_no=1 |
PMID | 15577423 |
ParticipantIDs | pubmed_primary_15577423 |
PublicationCentury | 2000 |
PublicationDate | 2004-December-15 |
PublicationDateYYYYMMDD | 2004-12-15 |
PublicationDate_xml | – month: 12 year: 2004 text: 2004-December-15 day: 15 |
PublicationDecade | 2000 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Journal of acquired immune deficiency syndromes (1999) |
PublicationTitleAlternate | J Acquir Immune Defic Syndr |
PublicationYear | 2004 |
SSID | ssj0016449 |
Score | 1.9758687 |
Snippet | To monitor the presence of genotypic HIV-1 variants circulating in eastern Cameroon, blood samples from 57 HIV-1-infected individuals attending 3 local health... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 1641 |
SubjectTerms | Adolescent Adult Amino Acid Sequence Cameroon - epidemiology Central African Republic - epidemiology Chad - epidemiology Child, Preschool Female Genetic Variation HIV Infections - epidemiology HIV Infections - virology HIV-1 - classification HIV-1 - genetics Humans Male Middle Aged Molecular Sequence Data Phylogeny Polymerase Chain Reaction Recombination, Genetic Rural Population Sequence Analysis, DNA Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - metabolism |
Title | Genetic diversity of HIV type 1 in rural eastern Cameroon |
URI | https://www.ncbi.nlm.nih.gov/pubmed/15577423 |
Volume | 37 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LSsQwFA2jgrgR32_Jwm2ladM2XcqgDKKzcmBwI3kNVpkq6Cz0E_xq703SscqIj00ZGijTntPTm-Tecwk5EmpUaKOKiHETR7wweaQgLois0PD1l0IwhgXOl_28N-Dnw2zY6by1spYmz-pYv86sK_kPqnAOcMUq2T8gO70onIDfgC8cAWE4_gpj9IxGw1XTTq7owYTOLawyV6vibDWwQQ-2POvKsYVQuf4mJJUa84IhBK2wagQrqtBfwhVnNs4G3tkJ4r3WEkLf2LGqAq9wK-pjOeDe3lZPPh13MqXhtb23XmIuLPZoMFPVB3F5we5OTqGru-rTmoRzPvRVmY2MJlkEn8esrbPe3CXwKWuJJszY2Ew19y7BGJHlacod9GiG4UrhvdC2QH4cO5QhPipw9_nn0S8-283QHJkrBGplH9d9wn4URI2lc94N9xVywrz95-y_5xo--Ut-mbK40OVqhSwHgOmJJ9Aq6dh6jSxehqyKdVIGHtEpj-jDiAKPKPKIMlrV1PGIBh7RhkcbZHB2etXtRaGlRqQh0C2jVCTOgy8pdJ6Cfmci1jxNNZdMSKUTNYoNSLqKJc8TzYoi5yW8tFJLDeIOs7FNMl8_1Hab0FJyJjPDYb4ac5sYoUTONCbdKF5yqXbIlr_pm0fvm3LTPI7db0f2yNIHnfbJwgheVHsAUd-zOnSAvAPMKkzb |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Genetic+diversity+of+HIV+type+1+in+rural+eastern+Cameroon&rft.jtitle=Journal+of+acquired+immune+deficiency+syndromes+%281999%29&rft.au=Ndembi%2C+Nicaise&rft.au=Takehisa%2C+Jun&rft.au=Zekeng%2C+Leopold&rft.au=Kobayashi%2C+Eiji&rft.date=2004-12-15&rft.issn=1525-4135&rft.volume=37&rft.issue=5&rft.spage=1641&rft_id=info:doi/10.1097%2F00126334-200412150-00019&rft_id=info%3Apmid%2F15577423&rft_id=info%3Apmid%2F15577423&rft.externalDocID=15577423 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1525-4135&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1525-4135&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1525-4135&client=summon |