Hierarchical Rules for Argonaute Loading in Drosophila

• Published in: Molecular cell Vol. 36; no. 3; pp. 445 - 456
• Main Authors: Czech, Benjamin, Zhou, Rui, Erlich, Yaniv, Brennecke, Julius, Binari, Richard, Villalta, Christians, Gordon, Assaf, Perrimon, Norbert, Hannon, Gregory J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.11.2009
• Subjects: 3' Untranslated Regions; Animals; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Argonaute 2 protein; Argonaute Proteins; Base Pairing; Blotting, Northern; Cell Line; Drosophila; Drosophila melanogaster - cytology; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Gene expression; Immunoprecipitation; MicroRNAs - chemistry; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Models, Biological; mRNA; Nucleic Acid Conformation; Protein Binding; PROTEINS; RNA; RNA Interference; RNA Precursors - genetics; RNA Precursors - metabolism; RNA, Double-Stranded - genetics; RNA, Double-Stranded - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - chemistry; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; RNA-Induced Silencing Complex - genetics; RNA-Induced Silencing Complex - metabolism; RNA-mediated interference; siRNA; Thermodynamics; RNA; PROTEINS

Drosophila Argonaute-1 and Argonaute-2 differ in function and small RNA content. AGO2 binds to siRNAs, whereas AGO1 is almost exclusively occupied by microRNAs. MicroRNA duplexes are intrinsically asymmetric, with one strand, the miR strand, preferentially entering AGO1 to recognize and regulate the...