Molecular Diagnosis, Prevalence and Importance of Zoonotic Vector-Borne Pathogens in Cuban Shelter Dogs—A Preliminary Study

The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examin...

Full description

Saved in:
Bibliographic Details
Published inPathogens (Basel) Vol. 9; no. 11; p. 901
Main Authors Díaz-Sánchez, Adrian Alberto, Corona-González, Belkis, Meli, Marina L., Roblejo-Arias, Lisset, Fonseca-Rodríguez, Osvaldo, Pérez Castillo, Anisleidy, Vega Cañizares, Ernesto, Lobo Rivero, Evelyn, Hofmann-Lehmann, Regina
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 28.10.2020
MDPI
Subjects
16S rRNA
Anaplasma phagocytophilum
Anaplasma platys
Anemia
Arachnids
assays
Bacteria
Blood
blood sampling
Borrelia burgdorferi
canine vector-borne diseases (CVBDs)
Canis
Cuba
Disease
Dogs
Ehrlichia canis
genes
Genetic diversity
genetic variation
Hematology
Hemoglobin
Infections
mixed infection
occurrence
Pathogens
Phylogeny
prevalence
Public health
real-time qPCR
Rhipicephalus sanguineus
Rickettsia felis
rRNA 16S
Samples
Serology
shelter dogs
Shelters
Statistical methods
strains
Thrombocytopenia
Ticks
Vectors (Biology)
Zoonoses
Venezuela
Asia
North America
Europe
Cuba
Rickettsia felis
16S rRNA
canine vector-borne diseases (CVBDs)
shelter dogs
Anaplasma platys
real-time qPCR
Ehrlichia canis
Online AccessGet full text

Cover

Abstract The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examined by molecular methods. Overall, 85 (85%; 95% CI: 77.88–92.12) dogs tested positive for at least one vector-borne pathogen using species-specific qPCR assays. Among the positive samples, E. canis was the most prevalent 62% (95% CI: 52.32–71.68), followed by A. platys 40% (95% CI: 30.23–49.77) and Rickettsia felis 27% (95% CI: 18.15–35.85), whereas 36% (95% CI: 26.43–45.57) showed co-infections. All samples were negative for A. phagocytophilum and B. burgdorferi s.l. The presence of 248 Rhipicephalus sanguineus ticks collected from the dogs was not statistically associated with the occurrence of infections. Thrombocytopenia was the most frequent haematological alteration found in PCR-positive dogs; it was statistically associated with the presence of E. canis, as well as co-infections (p < 0.05). The phylogenetic analyses of A. platys and E. canis based on 16S rRNA, groEL and gltA genes showed a low genetic diversity between Cuban strains. The present study demonstrates the high prevalence of vector-borne pathogens with zoonotic potential in shelter dogs from Cuba.
AbstractList The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examined by molecular methods. Overall, 85 (85%; 95% CI: 77.88–92.12) dogs tested positive for at least one vector-borne pathogen using species-specific qPCR assays. Among the positive samples, E. canis was the most prevalent 62% (95% CI: 52.32–71.68), followed by A. platys 40% (95% CI: 30.23–49.77) and Rickettsia felis 27% (95% CI: 18.15–35.85), whereas 36% (95% CI: 26.43–45.57) showed co-infections. All samples were negative for A. phagocytophilum and B. burgdorferi s.l. The presence of 248 Rhipicephalus sanguineus ticks collected from the dogs was not statistically associated with the occurrence of infections. Thrombocytopenia was the most frequent haematological alteration found in PCR-positive dogs; it was statistically associated with the presence of E. canis, as well as co-infections (p < 0.05). The phylogenetic analyses of A. platys and E. canis based on 16S rRNA, groEL and gltA genes showed a low genetic diversity between Cuban strains. The present study demonstrates the high prevalence of vector-borne pathogens with zoonotic potential in shelter dogs from Cuba.
The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys , Anaplasma phagocytophilum , Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examined by molecular methods. Overall, 85 (85%; 95% CI: 77.88-92.12) dogs tested positive for at least one vector-borne pathogen using species-specific qPCR assays. Among the positive samples, E. canis was the most prevalent 62% (95% CI: 52.32-71.68), followed by A. platys 40% (95% CI: 30.23-49.77) and Rickettsia felis 27% (95% CI: 18.15-35.85), whereas 36% (95% CI: 26.43-45.57) showed co-infections. All samples were negative for A. phagocytophilum and B. burgdorferi s.l. The presence of 248 Rhipicephalus sanguineus ticks collected from the dogs was not statistically associated with the occurrence of infections. Thrombocytopenia was the most frequent haematological alteration found in PCR-positive dogs; it was statistically associated with the presence of E. canis , as well as co-infections ( p &lt; 0.05). The phylogenetic analyses of A. platys and E. canis based on 16S rRNA, groEL and gltA genes showed a low genetic diversity between Cuban strains. The present study demonstrates the high prevalence of vector-borne pathogens with zoonotic potential in shelter dogs from Cuba.
The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examined by molecular methods. Overall, 85 (85%; 95% CI: 77.88-92.12) dogs tested positive for at least one vector-borne pathogen using species-specific qPCR assays. Among the positive samples, E. canis was the most prevalent 62% (95% CI: 52.32-71.68), followed by A. platys 40% (95% CI: 30.23-49.77) and Rickettsia felis 27% (95% CI: 18.15-35.85), whereas 36% (95% CI: 26.43-45.57) showed co-infections. All samples were negative for A. phagocytophilum and B. burgdorferi s.l. The presence of 248 Rhipicephalus sanguineus ticks collected from the dogs was not statistically associated with the occurrence of infections. Thrombocytopenia was the most frequent haematological alteration found in PCR-positive dogs; it was statistically associated with the presence of E. canis, as well as co-infections (p < 0.05). The phylogenetic analyses of A. platys and E. canis based on 16S rRNA, groEL and gltA genes showed a low genetic diversity between Cuban strains. The present study demonstrates the high prevalence of vector-borne pathogens with zoonotic potential in shelter dogs from Cuba.The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examined by molecular methods. Overall, 85 (85%; 95% CI: 77.88-92.12) dogs tested positive for at least one vector-borne pathogen using species-specific qPCR assays. Among the positive samples, E. canis was the most prevalent 62% (95% CI: 52.32-71.68), followed by A. platys 40% (95% CI: 30.23-49.77) and Rickettsia felis 27% (95% CI: 18.15-35.85), whereas 36% (95% CI: 26.43-45.57) showed co-infections. All samples were negative for A. phagocytophilum and B. burgdorferi s.l. The presence of 248 Rhipicephalus sanguineus ticks collected from the dogs was not statistically associated with the occurrence of infections. Thrombocytopenia was the most frequent haematological alteration found in PCR-positive dogs; it was statistically associated with the presence of E. canis, as well as co-infections (p < 0.05). The phylogenetic analyses of A. platys and E. canis based on 16S rRNA, groEL and gltA genes showed a low genetic diversity between Cuban strains. The present study demonstrates the high prevalence of vector-borne pathogens with zoonotic potential in shelter dogs from Cuba.
The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys , Anaplasma phagocytophilum , Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examined by molecular methods. Overall, 85 (85%; 95% CI: 77.88–92.12) dogs tested positive for at least one vector-borne pathogen using species-specific qPCR assays. Among the positive samples, E. canis was the most prevalent 62% (95% CI: 52.32–71.68), followed by A. platys 40% (95% CI: 30.23–49.77) and Rickettsia felis 27% (95% CI: 18.15–35.85), whereas 36% (95% CI: 26.43–45.57) showed co-infections. All samples were negative for A. phagocytophilum and B. burgdorferi s.l. The presence of 248 Rhipicephalus sanguineus ticks collected from the dogs was not statistically associated with the occurrence of infections. Thrombocytopenia was the most frequent haematological alteration found in PCR-positive dogs; it was statistically associated with the presence of E. canis , as well as co-infections ( p < 0.05). The phylogenetic analyses of A. platys and E. canis based on 16S rRNA, groEL and gltA genes showed a low genetic diversity between Cuban strains. The present study demonstrates the high prevalence of vector-borne pathogens with zoonotic potential in shelter dogs from Cuba.
The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including , , sensu lato, and spp. in shelter dogs from Cuba. Blood samples were collected from 100 shelter dogs and examined by molecular methods. Overall, 85 (85%; 95% CI: 77.88-92.12) dogs tested positive for at least one vector-borne pathogen using species-specific qPCR assays. Among the positive samples, was the most prevalent 62% (95% CI: 52.32-71.68), followed by 40% (95% CI: 30.23-49.77) and 27% (95% CI: 18.15-35.85), whereas 36% (95% CI: 26.43-45.57) showed co-infections. All samples were negative for and s.l. The presence of 248 ticks collected from the dogs was not statistically associated with the occurrence of infections. Thrombocytopenia was the most frequent haematological alteration found in PCR-positive dogs; it was statistically associated with the presence of , as well as co-infections ( < 0.05). The phylogenetic analyses of and based on rRNA, and genes showed a low genetic diversity between Cuban strains. The present study demonstrates the high prevalence of vector-borne pathogens with zoonotic potential in shelter dogs from Cuba.
Author Díaz-Sánchez, Adrian Alberto
Fonseca-Rodríguez, Osvaldo
Roblejo-Arias, Lisset
Lobo Rivero, Evelyn
Vega Cañizares, Ernesto
Hofmann-Lehmann, Regina
Corona-González, Belkis
Pérez Castillo, Anisleidy
Meli, Marina L.
AuthorAffiliation 5 Instituto Nacional de Higiene, Epidemiología y Microbiología (INHEM), La Habana 10300, Cuba
4 Department of Epidemiology and Global Health, Umeå University, 901 87 Umeå, Sweden; osvaldo.fonseca@umu.se
1 Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas 32700, Cuba; add196@usask.ca (A.A.D.-S.); bcorona@censa.edu.cu (B.C.-G.); lroblejo@censa.edu.cu (L.R.-A.); anisleidy@inhem.sld.cu (A.P.C.); evega@censa.edu.cu (E.V.C.); elobo@censa.edu.cu (E.L.R.)
2 Clinical Laboratory, Department of Clinical Diagnostics and Services, and Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland; mmeli@vetclinics.uzh.ch
3 Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK S7N 5E2, Canada
AuthorAffiliation_xml – name: 5 Instituto Nacional de Higiene, Epidemiología y Microbiología (INHEM), La Habana 10300, Cuba
– name: 3 Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK S7N 5E2, Canada
– name: 4 Department of Epidemiology and Global Health, Umeå University, 901 87 Umeå, Sweden; osvaldo.fonseca@umu.se
– name: 2 Clinical Laboratory, Department of Clinical Diagnostics and Services, and Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland; mmeli@vetclinics.uzh.ch
– name: 1 Centro Nacional de Sanidad Agropecuaria (CENSA), Carretera de Tapaste y Autopista Nacional, Apartado Postal 10, San José de las Lajas 32700, Cuba; add196@usask.ca (A.A.D.-S.); bcorona@censa.edu.cu (B.C.-G.); lroblejo@censa.edu.cu (L.R.-A.); anisleidy@inhem.sld.cu (A.P.C.); evega@censa.edu.cu (E.V.C.); elobo@censa.edu.cu (E.L.R.)
Author_xml – sequence: 1
  givenname: Adrian Alberto
  orcidid: 0000-0001-7743-7794
  surname: Díaz-Sánchez
  fullname: Díaz-Sánchez, Adrian Alberto
– sequence: 2
  givenname: Belkis
  orcidid: 0000-0002-2302-5361
  surname: Corona-González
  fullname: Corona-González, Belkis
– sequence: 3
  givenname: Marina L.
  orcidid: 0000-0002-3609-2416
  surname: Meli
  fullname: Meli, Marina L.
– sequence: 4
  givenname: Lisset
  orcidid: 0000-0002-3246-1026
  surname: Roblejo-Arias
  fullname: Roblejo-Arias, Lisset
– sequence: 5
  givenname: Osvaldo
  orcidid: 0000-0002-0253-5928
  surname: Fonseca-Rodríguez
  fullname: Fonseca-Rodríguez, Osvaldo
– sequence: 6
  givenname: Anisleidy
  surname: Pérez Castillo
  fullname: Pérez Castillo, Anisleidy
– sequence: 7
  givenname: Ernesto
  surname: Vega Cañizares
  fullname: Vega Cañizares, Ernesto
– sequence: 8
  givenname: Evelyn
  orcidid: 0000-0002-8103-4821
  surname: Lobo Rivero
  fullname: Lobo Rivero, Evelyn
– sequence: 9
  givenname: Regina
  orcidid: 0000-0001-9750-4296
  surname: Hofmann-Lehmann
  fullname: Hofmann-Lehmann, Regina
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33126690$$D View this record in MEDLINE/PubMed
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-177300$$DView record from Swedish Publication Index
BookMark eNqNks1u1DAQgCNUREvpnROKxIUDATt27PiCtGz5WamISoUeuFiOM951ldiLnRT1gMRD8IQ8CU63rborIeGLPfY3n2bkeZztOe8gy55i9IoQgV6v1bDyS3BRYIwEwg-ygxJxVqAa87175_3sKMYLlFaNpvhRtk8ILhkT6CD7-cl3oMdOhfzYqqXz0caX-WmAS9WB05Ar1-aLfu3DoKbQm_yb984PVufnoAcfirc-OMhPb6vJrcvnY6NcfraCboAk9sv459fv2aTtbG-dClf52TC2V0-yh0Z1EY5u9sPs6_t3X-Yfi5PPHxbz2UmhK4aHouE1oQJDbQBpSg1RVVuXiLWpawMGOEatxqQVDRbADOUUGc2VYmA01BrIYbbYeFuvLuQ62D6VIL2y8vrCh6VUIbXUgVS4bkRVAxO8oYZiVbYNiFIDrzAoZZKr2LjiD1iPzZbt2J7Prm1jP0rMOUEo8W82fIJ7aDW4IahuK237xdmVXPpLyZkoOauS4MWNIPjvI8RB9jZq6DrlwI9RllTUBFPC2H-gFaOYkKpM6PMd9MKPwaVfkGWVuJJSMlHP7hd_V_XtACUAbQAdfIwBzB2CkZzGVO6OaUphOynaDmqwfmrfdv9O_As3pvIz
CitedBy_id crossref_primary_10_1186_s13071_023_06098_0
crossref_primary_10_1016_j_actatropica_2022_106320
crossref_primary_10_1128_spectrum_03088_22
crossref_primary_10_3390_pathogens11090964
crossref_primary_10_1186_s13071_022_05426_0
Cites_doi 10.1016/j.ttbdis.2017.10.012
10.1007/s00436-015-4765-8
10.1603/0022-2585-41.3.533
10.1016/j.cvsm.2010.07.004
10.1016/j.ttbdis.2016.04.012
10.1128/AEM.71.10.6418-6422.2005
10.1016/j.cimid.2017.04.003
10.1016/j.meegid.2011.07.022
10.1093/molbev/msw054
10.1016/j.vetpar.2009.09.013
10.1016/j.ttbdis.2016.06.004
10.1016/j.vetpar.2007.12.030
10.1111/j.1469-0691.2010.03316.x
10.1128/jb.173.5.1576-1589.1991
10.1177/104063870601800215
10.1099/00207713-50-4-1449
10.1128/JCM.00366-06
10.1016/0304-4017(95)00904-3
10.1093/nar/gks400
10.4269/ajtmh.2005.73.1083
10.1016/j.ttbdis.2016.09.012
10.1136/vr.160.5.159
10.1196/annals.1374.016
10.1016/j.cimid.2015.02.002
10.1186/s13071-018-2914-5
10.1016/j.parint.2016.04.011
10.1093/molbev/mst010
10.1186/1746-6148-2-24
10.1016/j.ttbdis.2016.02.010
10.1093/nar/gkn201
10.1590/S1517-83822010000200019
10.1016/j.tvjl.2010.02.001
10.1016/j.prevetmed.2018.09.012
10.17236/sat00087
10.1016/j.meegid.2017.01.023
10.1016/j.pt.2010.01.007
10.1128/JCM.37.10.3390-3391.1999
10.1051/vetres:2003038
10.1186/s13071-016-1552-z
10.4269/ajtmh.14-0372
10.1186/1756-3305-3-26
10.1128/JCM.36.7.2035-2037.1998
10.1128/CMR.00032-13
10.1099/00207713-51-6-2145
10.1590/0001-3765201720150556
10.1186/1756-3305-6-103
10.1016/j.jiph.2017.01.002
10.1016/j.vetpar.2011.03.040
10.1055/s-2007-985142
10.1016/j.bjid.2012.06.020
10.1016/j.pt.2017.05.013
10.1016/j.vetpar.2016.04.017
10.1016/j.ttbdis.2019.05.009
10.1093/oxfordjournals.molbev.a026334
ContentType Journal Article
Copyright 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2020 by the authors. 2020
Copyright_xml – notice: 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2020 by the authors. 2020
DBID AAYXX
CITATION
NPM
7T7
8FD
8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
C1K
CCPQU
DWQXO
FR3
GNUQQ
HCIFZ
LK8
M7P
P64
PHGZM
PHGZT
PIMPY
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
7X8
7S9
L.6
5PM
ADHXS
ADTPV
AOWAS
D8T
D93
ZZAVC
DOA
DOI 10.3390/pathogens9110901
DatabaseName CrossRef
PubMed
Industrial and Applied Microbiology Abstracts (Microbiology A)
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One
ProQuest Central Korea
Engineering Research Database
ProQuest Central Student
SciTech Premium Collection
Biological Sciences
Biological Science Database
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic
Publicly Available Content Database
ProQuest One Academic Middle East (New)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
PubMed Central (Full Participant titles)
SWEPUB Umeå universitet full text
SwePub
SwePub Articles
SWEPUB Freely available online
SWEPUB Umeå universitet
SwePub Articles full text
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
PubMed
Publicly Available Content Database
ProQuest Central Student
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest Central
ProQuest One Applied & Life Sciences
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
Industrial and Applied Microbiology Abstracts (Microbiology A)
ProQuest Central (New)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Biological Science Database
ProQuest SciTech Collection
Biotechnology and BioEngineering Abstracts
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
ProQuest One Academic (New)
MEDLINE - Academic
AGRICOLA
AGRICOLA - Academic
DatabaseTitleList CrossRef

MEDLINE - Academic
AGRICOLA


PubMed
Publicly Available Content Database
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
Public Health
EISSN 2076-0817
ExternalDocumentID oai_doaj_org_article_a18b958e697b4f41a2dbe92ce751eaaf
oai_DiVA_org_umu_177300
PMC7692765
33126690
10_3390_pathogens9110901
Genre Journal Article
GeographicLocations Venezuela
Asia
North America
Europe
Cuba
GeographicLocations_xml – name: Asia
– name: Venezuela
– name: North America
– name: Europe
– name: Cuba
GroupedDBID 53G
5VS
8FE
8FH
AADQD
AAHBH
AAYXX
ADBBV
AFKRA
AFZYC
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
CCPQU
CITATION
DIK
GROUPED_DOAJ
HCIFZ
HYE
IHR
KQ8
LK8
M48
M7P
MODMG
M~E
OK1
PGMZT
PHGZM
PHGZT
PIMPY
PROAC
RPM
NPM
PQGLB
7T7
8FD
ABUWG
AZQEC
C1K
DWQXO
FR3
GNUQQ
P64
PKEHL
PQEST
PQQKQ
PQUKI
PRINS
7X8
7S9
L.6
5PM
ADHXS
ADRAZ
ADTPV
AOWAS
D8T
D93
IAO
IPNFZ
ITC
RIG
ZZAVC
PUEGO
ID FETCH-LOGICAL-c561t-b783491e8fe0c44f3a5d8206d901fefe710dc13d9b19e6f4740fc7aa6efce8ce3
IEDL.DBID M48
ISSN 2076-0817
IngestDate Wed Aug 27 01:24:31 EDT 2025
Thu Aug 21 06:42:07 EDT 2025
Thu Aug 21 14:39:57 EDT 2025
Fri Jul 11 11:31:13 EDT 2025
Fri Jul 11 10:20:38 EDT 2025
Fri Jul 25 12:11:47 EDT 2025
Mon Jul 21 05:51:51 EDT 2025
Tue Jul 01 04:15:35 EDT 2025
Thu Apr 24 22:57:39 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 11
Keywords Rickettsia felis
16S rRNA
canine vector-borne diseases (CVBDs)
shelter dogs
Anaplasma platys
real-time qPCR
Ehrlichia canis
Language English
License https://creativecommons.org/licenses/by/4.0
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c561t-b783491e8fe0c44f3a5d8206d901fefe710dc13d9b19e6f4740fc7aa6efce8ce3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0002-3246-1026
0000-0001-9750-4296
0000-0002-8103-4821
0000-0002-3609-2416
0000-0001-7743-7794
0000-0002-0253-5928
0000-0002-2302-5361
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.3390/pathogens9110901
PMID 33126690
PQID 2524524432
PQPubID 2032351
ParticipantIDs doaj_primary_oai_doaj_org_article_a18b958e697b4f41a2dbe92ce751eaaf
swepub_primary_oai_DiVA_org_umu_177300
pubmedcentral_primary_oai_pubmedcentral_nih_gov_7692765
proquest_miscellaneous_2498314366
proquest_miscellaneous_2456413352
proquest_journals_2524524432
pubmed_primary_33126690
crossref_primary_10_3390_pathogens9110901
crossref_citationtrail_10_3390_pathogens9110901
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20201028
PublicationDateYYYYMMDD 2020-10-28
PublicationDate_xml – month: 10
  year: 2020
  text: 20201028
  day: 28
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Pathogens (Basel)
PublicationTitleAlternate Pathogens
PublicationYear 2020
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Paulino (ref_29) 2018; 159
Thomson (ref_37) 2018; 11
Khrouf (ref_43) 2016; 7
Lima (ref_7) 2010; 41
Harrus (ref_17) 2011; 17
Santos (ref_34) 2009; 179
Noda (ref_45) 2016; 7
Soares (ref_27) 2017; 89
Santos (ref_22) 2016; 7
Alberti (ref_39) 2005; 71
Margos (ref_12) 2011; 11
Artimo (ref_58) 2012; 40
Belkahia (ref_20) 2017; 49
Traversa (ref_25) 2017; 51
Katoh (ref_60) 2013; 30
Otranto (ref_1) 2017; 33
(ref_11) 2010; 3
Chomel (ref_2) 2011; 179
Ordosgoitia (ref_16) 2019; 10
Liu (ref_26) 2016; 65
Stenos (ref_42) 2005; 73
Wagmann (ref_50) 2016; 158
Dolz (ref_5) 2017; 8
Martinez (ref_41) 2012; 16
Pusterla (ref_49) 1998; 36
Maggi (ref_23) 2013; 6
Marsilio (ref_38) 2006; 18
Little (ref_6) 2010; 40
Regnery (ref_55) 1991; 173
Oteo (ref_24) 2006; 44
Bulla (ref_33) 2004; 35
Parra (ref_10) 2014; 91
Woldehiwet (ref_13) 2010; 167
Foley (ref_52) 2007; 160
Castresana (ref_61) 2000; 17
Barlough (ref_53) 1996; 63
Parola (ref_15) 2013; 26
Inpankaew (ref_30) 2016; 9
(ref_31) 2008; 152
ref_35
Perez (ref_4) 2006; 1078
Leutenegger (ref_51) 1999; 37
Manzillo (ref_32) 2006; 48
Nicholson (ref_46) 2010; 26
Hamel (ref_18) 2015; 115
Kumar (ref_59) 2016; 33
Chisu (ref_36) 2018; 9
Meli (ref_48) 2007; 39
Navarrete (ref_21) 2018; 14
Dumler (ref_3) 2001; 51
Hernandez (ref_40) 2009; 12
Dahmani (ref_8) 2015; 39
Tsiodras (ref_14) 2017; 10
ref_47
Roux (ref_54) 2000; 50
Starkey (ref_28) 2016; 224
Harrus (ref_19) 2011; 187
Cardoso (ref_9) 2010; 183
Labruna (ref_56) 2004; 41
Hii (ref_44) 2020; 10
Johnson (ref_57) 2008; 36
References_xml – volume: 9
  start-page: 31
  year: 2018
  ident: ref_36
  article-title: GroEL typing and phylogeny of Anaplasma species in ticks from domestic and wild vertebrates
  publication-title: Ticks Tick Borne Dis.
  doi: 10.1016/j.ttbdis.2017.10.012
– volume: 115
  start-page: 489
  year: 2015
  ident: ref_18
  article-title: Parasites and vector-borne pathogens in client-owned dogs in Albania. Blood pathogens and seroprevalences of parasitic and other infectious agents
  publication-title: Parasitol. Res.
  doi: 10.1007/s00436-015-4765-8
– volume: 41
  start-page: 533
  year: 2004
  ident: ref_56
  article-title: Molecular Evidence for a Spotted Fever GroupRickettsiaSpecies in the TickAmblyomma longirostrein Brazil
  publication-title: J. Med. Entomol.
  doi: 10.1603/0022-2585-41.3.533
– volume: 40
  start-page: 1121
  year: 2010
  ident: ref_6
  article-title: Ehrlichiosis and Anaplasmosis in Dogs and Cats
  publication-title: Vet. Clin. Small Anim. Pract.
  doi: 10.1016/j.cvsm.2010.07.004
– volume: 7
  start-page: 938
  year: 2016
  ident: ref_22
  article-title: Molecular detection and characterization of Anaplasma platys in dogs and ticks in Cuba
  publication-title: Ticks Tick Borne Dis.
  doi: 10.1016/j.ttbdis.2016.04.012
– volume: 71
  start-page: 6418
  year: 2005
  ident: ref_39
  article-title: Equine and Canine Anaplasma phagocytophilum Strains Isolated on the Island of Sardinia (Italy) Are Phylogenetically Related to Pathogenic Strains from the United States
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.71.10.6418-6422.2005
– volume: 51
  start-page: 69
  year: 2017
  ident: ref_25
  article-title: Zoonotic intestinal parasites and vector-borne pathogens in Italian shelter and kennel dogs
  publication-title: Comp. Immunol. Microbiol. Infect. Dis.
  doi: 10.1016/j.cimid.2017.04.003
– volume: 183
  start-page: 232
  year: 2010
  ident: ref_9
  article-title: Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from the North of Portugal
  publication-title: Veter. J.
– volume: 11
  start-page: 1545
  year: 2011
  ident: ref_12
  article-title: Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato
  publication-title: Infect. Genet. Evol.
  doi: 10.1016/j.meegid.2011.07.022
– volume: 33
  start-page: 1870
  year: 2016
  ident: ref_59
  article-title: MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets
  publication-title: Mol. Biol. Evol.
  doi: 10.1093/molbev/msw054
– volume: 167
  start-page: 108
  year: 2010
  ident: ref_13
  article-title: The natural history of Anaplasma phagocytophilum
  publication-title: Veter. Parasitol.
  doi: 10.1016/j.vetpar.2009.09.013
– volume: 7
  start-page: 1057
  year: 2016
  ident: ref_45
  article-title: First report of spotted fever group Rickettsia in Cuba
  publication-title: Ticks Tick Borne Dis.
  doi: 10.1016/j.ttbdis.2016.06.004
– volume: 48
  start-page: 135
  year: 2006
  ident: ref_32
  article-title: Tick-transmitted diseases in dogs: Clinicopathological findings
  publication-title: Parassitologia
– volume: 152
  start-page: 173
  year: 2008
  ident: ref_31
  article-title: The brown dog tick, Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): From taxonomy to control
  publication-title: Veter. Parasitol.
  doi: 10.1016/j.vetpar.2007.12.030
– volume: 17
  start-page: 459
  year: 2011
  ident: ref_17
  article-title: Molecular detection of Ehrlichia canis, Anaplasma bovis, Anaplasma platys, Candidatus Midichloria mitochondrii and Babesia canis vogeli in ticks from Israel
  publication-title: Clin. Microbiol. Infect.
  doi: 10.1111/j.1469-0691.2010.03316.x
– volume: 173
  start-page: 1576
  year: 1991
  ident: ref_55
  article-title: Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes
  publication-title: J. Bacteriol.
  doi: 10.1128/jb.173.5.1576-1589.1991
– volume: 18
  start-page: 215
  year: 2006
  ident: ref_38
  article-title: Direct Identification ofEhrlichia Canisby a Novel Polymerase Chain Reaction Method and Molecular Analysis of the Citrate Synthase (gltA) Gene from Various Italian Strains
  publication-title: J. Veter. Diagn. Investig.
  doi: 10.1177/104063870601800215
– volume: 50
  start-page: 1449
  year: 2000
  ident: ref_54
  article-title: Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB (ompB)
  publication-title: Int. J. Syst. Evol. Microbiol.
  doi: 10.1099/00207713-50-4-1449
– volume: 44
  start-page: 2669
  year: 2006
  ident: ref_24
  article-title: Cluster of Cases of Human Rickettsia felis Infection from Southern Europe (Spain) Diagnosed by PCR
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.00366-06
– volume: 63
  start-page: 319
  year: 1996
  ident: ref_53
  article-title: Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus)
  publication-title: Vet. Parasitol.
  doi: 10.1016/0304-4017(95)00904-3
– volume: 40
  start-page: W597
  year: 2012
  ident: ref_58
  article-title: ExPASy: SIB bioinformatics resource portal
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gks400
– volume: 73
  start-page: 1083
  year: 2005
  ident: ref_42
  article-title: A highly sensitive and specific real-time PCR assay for the detection of spotted fever and Typhus group Rickettsiae
  publication-title: Am. J. Trop. Med. Hyg.
  doi: 10.4269/ajtmh.2005.73.1083
– volume: 8
  start-page: 36
  year: 2017
  ident: ref_5
  article-title: Novel genotype of Ehrlichia canis detected in samples of human blood bank donors in Costa Rica
  publication-title: Ticks Tick Borne Dis.
  doi: 10.1016/j.ttbdis.2016.09.012
– volume: 160
  start-page: 159
  year: 2007
  ident: ref_52
  article-title: Association between polyarthritis and thrombocytopenia and increased prevalence of vectorborne pathogens in Californian dogs
  publication-title: Vet. Rec.
  doi: 10.1136/vr.160.5.159
– volume: 1078
  start-page: 110
  year: 2006
  ident: ref_4
  article-title: Human Infection with Ehrlichia Canis Accompanied by Clinical Signs in Venezuela
  publication-title: Ann. N. Y. Acad. Sci.
  doi: 10.1196/annals.1374.016
– volume: 39
  start-page: 39
  year: 2015
  ident: ref_8
  article-title: Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria
  publication-title: Comp. Immunol. Microbiol. Infect. Dis.
  doi: 10.1016/j.cimid.2015.02.002
– volume: 11
  start-page: 350
  year: 2018
  ident: ref_37
  article-title: A new TaqMan method for the reliable diagnosis of Ehrlichia spp. in canine whole blood
  publication-title: Parasites Vectors
  doi: 10.1186/s13071-018-2914-5
– volume: 65
  start-page: 357
  year: 2016
  ident: ref_26
  article-title: Molecular survey of canine vector-borne diseases in stray dogs in Thailand
  publication-title: Parasitol. Int.
  doi: 10.1016/j.parint.2016.04.011
– volume: 30
  start-page: 772
  year: 2013
  ident: ref_60
  article-title: MAFFT multiple sequence alignment software version 7: Improvements in performance and usability
  publication-title: Mol. Biol. Evol.
  doi: 10.1093/molbev/mst010
– ident: ref_35
  doi: 10.1186/1746-6148-2-24
– volume: 7
  start-page: 653
  year: 2016
  ident: ref_43
  article-title: Molecular diagnosis of Rickettsia infection in patients from Tunisia
  publication-title: Ticks Tick Borne Dis.
  doi: 10.1016/j.ttbdis.2016.02.010
– volume: 36
  start-page: W5
  year: 2008
  ident: ref_57
  article-title: NCBI BLAST: A better web interface
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkn201
– volume: 41
  start-page: 381
  year: 2010
  ident: ref_7
  article-title: Molecular detection of Anaplasma platys in a naturally-infected cat in Brazil
  publication-title: Braz. J. Microbiol.
  doi: 10.1590/S1517-83822010000200019
– volume: 187
  start-page: 292
  year: 2011
  ident: ref_19
  article-title: Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia canis): An overview
  publication-title: Vet. J.
  doi: 10.1016/j.tvjl.2010.02.001
– volume: 159
  start-page: 135
  year: 2018
  ident: ref_29
  article-title: Epidemiology of Ehrlichia canis in healthy dogs from the Southeastern region of the state of Rio de Janeiro, Brazil
  publication-title: Prev. Veter. Med.
  doi: 10.1016/j.prevetmed.2018.09.012
– ident: ref_47
– volume: 158
  start-page: 691
  year: 2016
  ident: ref_50
  article-title: Detection of ‘Candidatus Neoehrlichia mikurensis’ and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries
  publication-title: Schweizer Archiv für Tierheilkunde
  doi: 10.17236/sat00087
– volume: 49
  start-page: 226
  year: 2017
  ident: ref_20
  article-title: Anaplasma platys-like strains in ruminants from Tunisia
  publication-title: Infect. Genet. Evol.
  doi: 10.1016/j.meegid.2017.01.023
– volume: 26
  start-page: 205
  year: 2010
  ident: ref_46
  article-title: The increasing recognition of rickettsial pathogens in dogs and people
  publication-title: Trends Parasitol.
  doi: 10.1016/j.pt.2010.01.007
– volume: 37
  start-page: 3390
  year: 1999
  ident: ref_51
  article-title: Molecular Evidence of Coinfection of Ticks with Borrelia burgdorferi Sensu Lato and the Human Granulocytic Ehrlichiosis Agent in Switzerland
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.37.10.3390-3391.1999
– volume: 14
  start-page: 170
  year: 2018
  ident: ref_21
  article-title: Serological and molecular diagnosis of Ehrlichia canis and associated risk factors in dogs domiciled in western Cuba
  publication-title: Vet. Parasitol. Reg. Stud. Rep.
– volume: 35
  start-page: 141
  year: 2004
  ident: ref_33
  article-title: The relationship between the degree of thrombocytopenia and infection with Ehrlichia canis in an endemic area
  publication-title: Veter. Res.
  doi: 10.1051/vetres:2003038
– volume: 9
  start-page: 253
  year: 2016
  ident: ref_30
  article-title: Canine vector-borne pathogens in semi-domesticated dogs residing in northern Cambodia
  publication-title: Parasites Vectors
  doi: 10.1186/s13071-016-1552-z
– volume: 91
  start-page: 1161
  year: 2014
  ident: ref_10
  article-title: Molecular Evidence of Anaplasma platys Infection in Two Women from Venezuela
  publication-title: Am. J. Trop. Med. Hyg.
  doi: 10.4269/ajtmh.14-0372
– volume: 3
  start-page: 26
  year: 2010
  ident: ref_11
  article-title: Biology and ecology of the brown dog tick, Rhipicephalus sanguineus
  publication-title: Parasites Vectors
  doi: 10.1186/1756-3305-3-26
– volume: 12
  start-page: 101
  year: 2009
  ident: ref_40
  article-title: The ixodid ticks (Acari: Ixodidae) of Cuba
  publication-title: Syst. Appl. Acarol.
– volume: 36
  start-page: 2035
  year: 1998
  ident: ref_49
  article-title: Identification of a Granulocytic Ehrlichia Strain Isolated from a Horse in Switzerland and Comparison with Other Rickettsiae of the Ehrlichia phagocytophila Genogroup
  publication-title: J. Clin. Microbiol.
  doi: 10.1128/JCM.36.7.2035-2037.1998
– volume: 26
  start-page: 657
  year: 2013
  ident: ref_15
  article-title: Update on Tick-Borne Rickettsioses around the World: A Geographic Approach
  publication-title: Clin. Microbiol. Rev.
  doi: 10.1128/CMR.00032-13
– volume: 51
  start-page: 2145
  year: 2001
  ident: ref_3
  article-title: Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: Unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ’HGE agent’ as subjective synonyms of Ehrlichia phagocytophila
  publication-title: Int. J. Syst. Evol. Microbiol.
  doi: 10.1099/00207713-51-6-2145
– volume: 89
  start-page: 301
  year: 2017
  ident: ref_27
  article-title: Molecular survey of Anaplasma platys and Ehrlichia canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil
  publication-title: Anais da Academia Brasileira de Ciências
  doi: 10.1590/0001-3765201720150556
– volume: 6
  start-page: 103
  year: 2013
  ident: ref_23
  article-title: Co-infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian
  publication-title: Parasites Vectors
  doi: 10.1186/1756-3305-6-103
– volume: 10
  start-page: 819
  year: 2017
  ident: ref_14
  article-title: Fatal human anaplasmosis associated with macrophage activation syndrome in Greece and the Public Health response
  publication-title: J. Infect. Public Health
  doi: 10.1016/j.jiph.2017.01.002
– volume: 179
  start-page: 294
  year: 2011
  ident: ref_2
  article-title: Tick-borne infections in dogs—An emerging infectious threat
  publication-title: Veter. Parasitol.
  doi: 10.1016/j.vetpar.2011.03.040
– volume: 39
  start-page: 548
  year: 2007
  ident: ref_48
  article-title: Quantitative Real-time PCR for the Measurement of 11?-HSD1 and 11?-HSD2 mRNA Levels in Tissues of Healthy Dogs
  publication-title: Horm. Metab. Res.
  doi: 10.1055/s-2007-985142
– volume: 10
  start-page: 1
  year: 2020
  ident: ref_44
  article-title: Domestic dogs are mammalian reservoirs for the emerging zoonosis flea-borne spotted fever, caused by Rickettsia felis
  publication-title: Sci. Rep.
– volume: 16
  start-page: 405
  year: 2012
  ident: ref_41
  article-title: Serological evidences suggest Borrelia burgdorferi sensu lato infection in Cuba
  publication-title: Braz. J. Infect. Dis.
  doi: 10.1016/j.bjid.2012.06.020
– volume: 33
  start-page: 813
  year: 2017
  ident: ref_1
  article-title: Zoonotic Parasites of Sheltered and Stray Dogs in the Era of the Global Economic and Political Crisis
  publication-title: Trends Parasitol.
  doi: 10.1016/j.pt.2017.05.013
– volume: 224
  start-page: 7
  year: 2016
  ident: ref_28
  article-title: Prevalence of vector-borne pathogens in dogs from Haiti
  publication-title: Vet. Parasitol.
  doi: 10.1016/j.vetpar.2016.04.017
– volume: 179
  start-page: 145
  year: 2009
  ident: ref_34
  article-title: Molecular evaluation of the incidence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs from Ribeirão Preto, Brazil
  publication-title: Veter. J.
– volume: 10
  start-page: 995
  year: 2019
  ident: ref_16
  article-title: Fatal Rickettsia rickettsii infection in a child, Northwestern Colombia, 2017
  publication-title: Ticks Tick Borne Dis.
  doi: 10.1016/j.ttbdis.2019.05.009
– volume: 17
  start-page: 540
  year: 2000
  ident: ref_61
  article-title: Selection of Conserved Blocks from Multiple Alignments for Their Use in Phylogenetic Analysis
  publication-title: Mol. Biol. Evol.
  doi: 10.1093/oxfordjournals.molbev.a026334
SSID ssj0000800817
Score 2.1933413
Snippet The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys, Anaplasma phagocytophilum, Borrelia...
The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including , , sensu lato, and spp. in shelter dogs from Cuba. Blood...
The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys , Anaplasma phagocytophilum , Borrelia...
The present study aimed to determine the prevalence of zoonotic vector-borne pathogens, including Anaplasma platys , Anaplasma phagocytophilum , Borrelia...
SourceID doaj
swepub
pubmedcentral
proquest
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 901
SubjectTerms 16S rRNA
Anaplasma phagocytophilum
Anaplasma platys
Anemia
Arachnids
assays
Bacteria
Blood
blood sampling
Borrelia burgdorferi
canine vector-borne diseases (CVBDs)
Canis
Cuba
Disease
Dogs
Ehrlichia canis
genes
Genetic diversity
genetic variation
Hematology
Hemoglobin
Infections
mixed infection
occurrence
Pathogens
Phylogeny
prevalence
Public health
real-time qPCR
Rhipicephalus sanguineus
Rickettsia felis
rRNA 16S
Samples
Serology
shelter dogs
Shelters
Statistical methods
strains
Thrombocytopenia
Ticks
Vectors (Biology)
Zoonoses
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1di9QwFA2yIPgifltdJYIIgmUnTZqPx9kdl1VYEXSXxZeSpIkzspvKzPRhHgR_hL_QX2Ju2g5blfXFxzZpaO-9aU6Sm3MQem6NotJInyvKWc6o0blimuWSMU8LqkVp4aDw8Tt-dMLenpVnl6S-ICesowfuDLeniTSqlI4rYZhnRBe1caqwTpTEae3h7xvHvEuTqS89DpJEdPuSNM7rIb963kSfrBRwbPYaMMM4lOj6_4Yx_0yVHBGKpkHo8Ba62aNHPO3e-ja65sIddL3Tk9zcRd-OB7FbPOtS6BarVxhYmnQ6W4R1qPGbiwS54bLx-FPThCa2hk_T8n2-3yyDw--Hz8CLgA9aowP-MHewsY5nzefVz-8_ptDseZIEW24wZCNu7qGTw9cfD47yXl8htxE1rXMDIhuKOOndxIJvdFkDnXsdzeOddxF81JbQWhmiHPdMsIm3QmvuvHXSOnof7YQmuIcIg4I1KGhIzh0ro8sntebaahC_0oTVGdobrF3ZnnwcNDDOqzgJAf9Uv_snQy-3T3ztiDeuqLsPDtzWA8rsdCMGUtUHUvWvQMrQ7uD-qu_Hq6ooYWeaMVpk6Nm2OPZA2FbRwTVtrAOMPATOrl1VR0kaoSnnGXrQRdT2bSklESWpSYbEKNZGnzMuCYt5YgIXXBWClxl60UXl6JHZ4nSaLNBetBURIEzw6H8Y6jG6UcCiQxzAC7mLdtbL1j2JyGxtnqZO-AszpD3k
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1daxQxFL3oFkEQ0ap1tEoEEQSHbiaZTPIku92WKrQUtaX4MmQySXehndT9eNgHwR_hL_SXmDsfK6uyjzuTGTa5N8mZe2_OAXhtCsVkIV2smOAxZ4WOFdc8lpw7ljCdpQYPCh-fiKMz_vEivWgDbrO2rLJbE-uFuvQGY-R7SYo5Qs5Z8v7mW4yqUZhdbSU0bsNWWIKl7MHW8ODk9NMqyoJ4SNKsyU-y8H2PddZjH2wzU8i12WrBdPtRTdv_P6z5b8nkGrFovRkdPoD7LYokg8bsD-GWrbbhTqMrudyGe00wjjRnjB7B9-NOBJeMmtK6yewdQfYmXZ85IroqyYfrGorjT-_IV-8rH95Ozuuwfjz008qS065bZFKR_UWhK_J5bDHhTkb-cvbrx88BvvaqlgqbLglWKS4fw9nhwZf9o7jVXYhNQFPzuEDxDUWtdLZv0GY6LZHmvQzD5ayzAZSUhrJSFVRZ4XjG-85kWgvrjJXGsifQq3xlnwJBZWtU1pBCWJ4GV-iXWmijURRLU15GsNeNfm5aUnLUxrjKw8cJ2iv_214RvF09cdMQcmxoO0SDrtohlXZ9wU8v83Zm5prKQqXSCpUV3HGqk7KwKjE2S6nV2kWw27lD3s7vWf7HGyN4tbodZiamW3Rl_SK0QaYeimfaNrVRkgXIKkQEO42Hrf4tYzSgJ9WPIFvzvbXurN-pJuOaITwTKslEGsGbxkvXHhlNzgf1CCyuFznNULDg2eY-Poe7CYYZwpadyF3ozacL-yJgsXnxsp1wvwEt0Dpn
  priority: 102
  providerName: ProQuest
Title Molecular Diagnosis, Prevalence and Importance of Zoonotic Vector-Borne Pathogens in Cuban Shelter Dogs—A Preliminary Study
URI https://www.ncbi.nlm.nih.gov/pubmed/33126690
https://www.proquest.com/docview/2524524432
https://www.proquest.com/docview/2456413352
https://www.proquest.com/docview/2498314366
https://pubmed.ncbi.nlm.nih.gov/PMC7692765
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-177300
https://doaj.org/article/a18b958e697b4f41a2dbe92ce751eaaf
Volume 9
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1di9QwFA2yi-CL-G3XdYgggmB10qRp8iAys7PLKsyyqLMsvpQ0TXZGZludmYLzIPgj_IX-EnPTdqQ6LL5Np2lo770h5ya55yD0VGeSikzYUFLOQkYzFUqmWCgYszSiKok1FAqPT_jxhL07j8__lEc3BlxuTe1AT2qymL_89nX9xg3415BxupQdjk5PS2fupQT6TCjm2nXzUgJ6BuMG7H9usJHwEryRS95D-F3vW27tpDNPeTr_bRj036OUHcJRP0kd3UI3G3SJB3U43EbXTHEHXa_1Jtd30fdxK4aLR_URu9nyBQYWJ-Vrj7Aqcvz20lsDLkuLP5VlUbre8Jlf3g-H5aIw-LT9DDwr8EGVqQJ_mBrYeMej8mL568fPAXQ795JhizWG04rre2hydPjx4Dhs9BdC7VDVKsxAhEMSI6zpa_CdinOge8-deayxxoGTXBOay4xIwy1LWN_qRClurDZCG3of7RRlYR4iDArXoLAhODcsdiHRzxVXWoE4liIsD9Cr1tqpbsjJQSNjnrokBfyT_u2fAD3fPPGlJua4ou0QHLhpB5Ta_o9ycZE2IzRVRGQyFobLJGOWERXlmZGRNklMjFI2QPut-9M2TNMohp1rxmgUoCeb226EwraLKkxZuTbA2EOgtu2qNlJQB105D9CDOqI2b0spcShK9gOUdGKt8zndO8Vs6pnCEy6jhMcBelZHZeeR0exs4C1QXVYpSUC4YO8_XvARuhHBmoObvyOxj3ZWi8o8dsBslfXQ7vDw5PR9zy9s9Pzo-w18cz8m
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3dbtMwFD4anRBICMH4CwwwEiAhLVoSO058gVC7bmrZWk2wTRM3wXGctdKWjKYV6gUSD8Fz8FA8CT75KSqg3u0yiW3FOcfHX3x-PoCXKhY0jMPUFpQzm9FY2oJJZoeMpdSjMvAVJgoPhrx3zN6f-qdr8LPJhcGwysYmloY6yRWekW97PvoIGaPeu8svNrJGoXe1odCo1GJfz7-aX7bibb9r5PvK8_Z2j3Z6ds0qYCuDFaZ2jNQSwtVhqh2FbyT9BIuYJ2ZnTHWqzZabKJcmInaF5ikLmJOqQEquU6VDpakZ9xqsM8odrwXrnd3h4YfFqQ7ir9ANKn8opcLBuO5RbnShEFjbs-aeafa_kibgf9j23xDNpUKm5ea3dwdu16iVtCs1uwtrOtuA6xWP5XwDblWHf6TKaboH3wYN6S7pVqF842KLYLUoWeY4EZklpH9RQn-8zFPyKc-z3IxOTko3gt3JJ5kmh820yDgjO7NYZuTjSKODn3Tzs-LX9x9tHPa8pCabzAlGRc7vw_GVSOQBtLI804-AIJM2MnmEnGvmG9VzEsmlkkjCJV2WWLDdfP1I1UXQkYvjPDI_Qyiv6G95WfBm0eOyKgCyom0HBbpoh6W7yxv55CyqLUEk3TAWfqi5CGKWMld6SayFp3Tgu1rK1ILNRh2i2p4U0R_tt-DF4rGxBOjekZnOZ6YNVgZyMYduVRsRUgORObfgYaVhi7el1DVoTTgWBEu6tzSd5SfZeFRWJA-48ALuW_C60tKlLt3xSbv8ArOLWeQGSJDwePUcn8ON3tHgIDroD_efwE0PjzgMXPDCTWhNJzP91ODAafysXnwEPl_1ev8NM2h5Bg
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3dbtMwFD4anUBICMH4CwwwEiAhLWoTO058gVC7rloZqypg08RNcBx7rbQloz9CvUDiIXgaHocnwSc_RQXUu10mcaw45_j42Oec7wN4rhJBoyQyrqCcuYwm0hVMMjdizFCfyjBQWCh8OOD7R-ztSXCyAT_rWhhMq6xtYmGo01zhGXnTDzBGyBj1m6ZKixh2e28uvrjIIIWR1ppOo1SRA734ardv09f9rpX1C9_v7X3c3XcrhgFXWb9h5iZIMyE8HRndUvh1MkgR0Dy1q6TRRtvlN1UeTUXiCc0NC1nLqFBKro3SkdLU9nsFNkPcFTVgs7M3GL5fnvCgLxZ5YRkbpVS0MMd7lFu9mArE-ax4aOq1sKAM-J-f-2-65gqoabEQ9m7BzcqDJe1S5W7Dhs624GrJabnYghvlQSAp65vuwLfDmoCXdMu0vvF0hyBylCzqnYjMUtI_L7YBeJkb8inPs9z2To6LkILbySeZJsN6WGSckd15IjPyYaQx2E-6-en01_cfbez2rKApmywIZkgu7sLRpUjkHjSyPNMPgCCrNrJ6RJxrFlg1bKWSSyWRkEt6LHWgWf_9WFWA6MjLcRbbjRHKK_5bXg68Wr5xUYKBrGnbQYEu2yGMd3Ejn5zGlVWIpRclIog0F2HCDPOknyZa-EqHgaelNA5s1-oQV7ZlGv-ZCQ48Wz62VgFDPTLT-dy2QZQgD-vp1rUREbXuMucO3C81bPm1lHrWcxMtB8IV3VsZzuqTbDwq0MlDLvyQBw68LLV05ZXu-Lhd_IH5-Tz2QiRLeLh-jE_hmp3n8bv-4OARXPfxtMN6Dn60DY3ZZK4fW5dwljyp5h6Bz5c93X8DY6N9Ow
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Molecular+Diagnosis%2C+Prevalence+and+Importance+of+Zoonotic+Vector-Borne+Pathogens+in+Cuban+Shelter+Dogs%E2%80%94A+Preliminary+Study&rft.jtitle=Pathogens+%28Basel%29&rft.au=D%C3%ADaz-S%C3%A1nchez%2C+Adrian+Alberto&rft.au=Corona-Gonz%C3%A1lez%2C+Belkis&rft.au=Meli%2C+Marina+L&rft.au=Roblejo-Arias%2C+Lisset&rft.date=2020-10-28&rft.issn=2076-0817&rft.eissn=2076-0817&rft.volume=9&rft.issue=11&rft_id=info:doi/10.3390%2Fpathogens9110901&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2076-0817&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2076-0817&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2076-0817&client=summon