Establishment of a human microbiome- and immune system-reconstituted dual-humanized mouse model

• Published in: Experimental Animals Vol. 72; no. 3; pp. 402 - 412
• Main Authors: Ka, Yuyo, Ito, Ryoji, Nozu, Ryoko, Tomiyama, Kayo, Ueno, Masami, Ogura, Tomoyuki, Takahashi, Riichi
• Format: Journal Article
• Language: English
• Published: Japan Japanese Association for Laboratory Animal Science 01.01.2023; Japan Science and Technology Agency
• Subjects: Animal models; CD3 antigen; CD34 antigen; CD34+ cells; Cell proliferation; fecal microbiota transplantation; Flow cytometry; germ-free; Germfree; Hematopoietic stem cells; Homeostasis; humanized mice; Immune system; Immunodeficiency; Immunology; In vivo methods and tests; Intestinal microflora; Lymphocytes; Lymphocytes T; Maintenance; Microbiomes; Microbiota; Microorganisms; NOD/Shi-scid-IL2rγnull (NOG) mice; Original; Stem cells; Therapeutic targets; germ-free; NOD/Shi-scid-IL2rγnull (NOG) mice; fecal microbiota transplantation; humanized mice; CD34+ cells

Humanized mice are widely used to study the human immune system in vivo and investigate therapeutic targets for various human diseases. Immunodeficient NOD/Shi-scid-IL2rγnull (NOG) mice transferred with human hematopoietic stem cells are a useful model for studying human immune systems and analyzing...