Changes in lipoprotein lipase modulate tissue energy supply during stress

• Published in: Journal of applied physiology (1985) Vol. 99; no. 4; pp. 1343 - 1351
• Main Authors: Ricart-Jane, David, Cejudo-Martin, Pilar, Peinado-Onsurbe, Julia, Lopez-Tejero, M. Dolores, Llobera, Miquel
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01.10.2005; American Physiological Society
• Subjects: Adipose Tissue - metabolism; Adipose Tissue - pathology; Adrenal Glands - enzymology; Animals; Biological and medical sciences; Energy Metabolism; Enzymes; Epididymis; Fatty acids; Fundamental and applied biological sciences. Psychology; Immobilization; Lipoprotein Lipase - blood; Lipoprotein Lipase - genetics; Lipoprotein Lipase - metabolism; Male; Mesentery; Muscle, Skeletal - enzymology; Myocardium - enzymology; Organ Size; Proteins; Rats; Rats, Wistar; Retroperitoneal Space; Ribonucleic acid; RNA; RNA, Messenger - metabolism; Rodents; Stress; Stress, Physiological - enzymology; Stress, Physiological - etiology; Stress, Physiological - metabolism; Stress, Physiological - pathology; Time Factors; Weight Loss; White adipose tissue; Enzyme; lipoprotein lipase messenger ribonucleic acid; Immobilization; Esterases; Lipoprotein lipase; Striated muscle; Stress; Carboxylic ester hydrolases; Blood flow; immobilization stress; Vertebrata; Mammalia; muscle; Hydrolases; Hemodynamics
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00971.2004

Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain Submitted 3 September 2004 ; accepted in final form 6 June 2005 We studied the variations caused by stress in lipoprotein lipase (LPL) activity, LPL-mRNA, and local blood flow in LPL-rich tissues in the rat. Stress was produced by body immobilization (Immo): the rat's limbs were taped to metal mounts, and its head was placed in a plastic tube. Chronic stress (2 h daily of Immo) decreased total LPL activity in mesenteric and epididymal white adipose tissue (WAT) and was accompanied by a weight reduction of these tissues. In limb muscle, heart, and adrenals, total LPL activity and mRNA levels increased, and, in plasma, LPL activity and mass also increased. Acute stress (30-min Immo) caused a decrease in total LPL activity only in retroperitoneal WAT and an increase in preheparin plasma active LPL, but the overall weight of this tissue did not vary significantly. We propose an early release of the enzyme from this tissue into the bloodstream by some unknown extracellular pathways or other local mechanisms. These changes in this key energy-regulating enzyme are probably induced by catecholamines. They modify the flow of energy substrates between tissues, switching the WAT from importer to exporter of free fatty acids and favoring the uptake by muscle of circulating triacylglycerides for energy supply. Moreover, we found that acute stress almost doubled blood flow in all WAT studied, favoring the export of free fatty acids. immobilization stress; lipoprotein lipase messenger ribonucleic acid; muscle; white adipose tissue; blood flow Address for reprint requests and other correspondence: M. Llobera, Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Avda. Diagonal 645, E-08028 Barcelona, Spain (e-mail: millobera{at}ub.edu )