海洋红酵母RH1菌株发酵培养条件的研究

该研究比较了不同碳源、氮源、无机盐对海洋红酵母菌（Rhodotorula sp.）RH1菌株发酵产量的影响,通过葡萄糖、蛋白胨、酵母膏和硫酸镁（MgSO4）4因素3水平的正交试验,确定了RH1菌株的最优培养基为蛋白胨10 g.L-1,酵母膏15 g.L-1,葡萄糖20 g.L-1,MgSO40.25 g.L-1,磷酸二氢钾（KH2PO4）0.25 g.L-1,氯化钠（NaCl）10 g.L-1。结果显示,该菌株最佳摇瓶发酵条件为接种量10%,初始pH 5.0,摇瓶装液量80 mL/500 mL三角瓶,培养温度28℃,经48 h培养,菌量可达10.46×108cfu.mL-1,比优化条件前提高2...

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Bibliographic Details
Published in南方水产科学 Vol. 8; no. 1; pp. 67 - 74
Main Author 杨莺莺 陈永青 杨铿 梁晓华 徐创文 梁润捷 洪敏娜
Format Journal Article
LanguageChinese
Published 中国水产科学研究院南海水产研究所,广东广州,510300 2012
Subjects
优化
培养条件
海洋红酵母
优化
海洋红酵母
培养条件
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ISSN2095-0780
DOI10.3969/j.issn.2095-0780.2012.01.011

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Summary:该研究比较了不同碳源、氮源、无机盐对海洋红酵母菌（Rhodotorula sp.）RH1菌株发酵产量的影响,通过葡萄糖、蛋白胨、酵母膏和硫酸镁（MgSO4）4因素3水平的正交试验,确定了RH1菌株的最优培养基为蛋白胨10 g.L-1,酵母膏15 g.L-1,葡萄糖20 g.L-1,MgSO40.25 g.L-1,磷酸二氢钾（KH2PO4）0.25 g.L-1,氯化钠（NaCl）10 g.L-1。结果显示,该菌株最佳摇瓶发酵条件为接种量10%,初始pH 5.0,摇瓶装液量80 mL/500 mL三角瓶,培养温度28℃,经48 h培养,菌量可达10.46×108cfu.mL-1,比优化条件前提高23.9%。还进行了RH1菌株25 L发酵罐扩大培养试验,在接种量为8%、初始pH 5.0、搅拌速率350～400 r.min-1、通气量9 L.min-1、温度28℃的条件下,经28 h的培养,菌量可达33.6×108cfu.mL-1。预计通过连续补料的方式进行培养,有望进一步提高菌量。
Bibliography:YANG Yingying,CHENG Yongqing,YANG Keng,LIANG Xiaohua, XU Chuangwen,LIANG Runjie,HONG Minna（South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China）
Rhodotorula sp.； culture conditions； optimization
44-1683/S
We compared the effects of different carbon sources,nitrogen sources and inorganic salts on fermentation yield of oceanic red yeast（Rhodotorula sp.）RH1.By 4 factors and 3 levels orthogonal test,the optimum fermentation conditions for RH1 were determined： peptone 10 g·L-1,yeast extract 15 g·L-1,glucose 20 g·L-1,MgSO4 0.25 g·L-1,KH2PO4 0.25 g·L-1,NaCl 10 g·L-1,inoculation amount 10%,initial pH 5.0,temperature 28 ℃,80 mL medium in 500 mL triangle bottle,and fermentation time 48 h.Under above conditions,the cell biomass of RH1 can reach 10.46×108 cfu·mL-1,which increases by 23.9% as compared with the fermentation before optimization.An experiment of expanded culture is also conducted in a 25 L fermentation tank.The cell biomass of RH1 increased to 33.6×108 cfu·mL-1
ISSN:2095-0780
DOI:10.3969/j.issn.2095-0780.2012.01.011