Improvement of Lactose Digestion in Humans by Ingestion of Unfermented Milk Containing Bifidobacterium longum

• Published in: Journal of dairy science Vol. 79; no. 5; pp. 750 - 757
• Main Authors: Jiang, Tianan, Mustapha, Azlin, Savaiano, Dennis A.
• Format: Journal Article
• Language: English
• Published: Savoy, IL Elsevier Inc 01.05.1996; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Adult; Animals; BACTERIA; BETA GALACTOSIDASA; BETA GALACTOSIDASE; beta-Galactosidase - metabolism; BIFIDOBACTERIUM; Bifidobacterium - metabolism; Bifidobacterium longum; Biological and medical sciences; breath hydrogen test; Breath Tests; CONTEO DE CELULAS; culture media; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; Digestion; Double-Blind Method; enzyme activity; Female; Fermentation; Gastroenterology. Liver. Pancreas. Abdomen; GENERO HUMANO; GENRE HUMAIN; GLUCOSA; GLUCOSE; Glucose - metabolism; HIDROGENO; Humans; hydrogen; Hydrogen - metabolism; HYDROGENE; LACTOSA; LACTOSE; Lactose - metabolism; lactose digestion; lactose intolerance; Lactose Intolerance - metabolism; Lactose Intolerance - therapy; LAIT; LECHE; Male; Medical sciences; MEDIO DE CULTIVO; metabolism; microbiology; MILIEU DE CULTURE; milk; Milk - microbiology; NUMERATION CELLULAIRE; Other diseases. Semiology; plate count; SINTOMAS; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; strain differences; SYMPTOME; therapy; TRASTORNOS DIGESTIVOS; TROUBLE DIGESTIF; B6 milk; ONPG; lactose digestion; Bifidobacterium longum; Com-B6 milk; breath hydrogen test; 15708 milk; m-MRS; β-gal; Human; Actinomycetaceae; Food intolerance; Enzyme; Digestion; Feeding; Actinomycetales; Lactose; Enzymatic activity; Intestinal malabsorption; Glycosidases; Digestive diseases; Bacteria; Hydrolases; Intestinal disease; Actinomycetes; O-Glycosidases; β-Galactosidase; Milk
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(96)76422-6

Fifteen lactose malabsorbers were studied to evaluate the effects of consumption of milk containing different strains of Bifidobacterium longum on lactose digestion. Influences of different growth substrates, bile sensitivity, and lactose transport on lactose digestion by bifidobacteria were also investigated. Lactose malabsorption was determined by measuring breath hydrogen excretion of subjects fed four different test milks (three of which contained 5 × 108 cfu/ml of B. longum) on 4 different d using a randomized, double-blinded trial. Test milks included 1) 400ml of lowfat milk (control), 2) 400ml of milk containing B. longum B6 that had been grown with lactose, 3) 400ml of milk containing B. longum B6 grown with lactose plus glucose, or 4) 400ml of milk containing B. longum ATCC 15708 grown with lactose. β-Galactosidase activity was highest in milk containing B6 grown with lactose but was extremely low in milk containing B6 grown with lactose and glucose. Consumption of milk containing B6 grown with lactose resulted in significantly less hydrogen production and flatulence than occurring after consumption of control milk or the milk containing B6 grown with both lactose and glucose. Hydrogen production after ingestion of 15708 was also significantly lower than hydrogen production after ingestion of the control milk. We concluded that milks containing B. longum might reduce breath hydrogen response and symptoms from lactose malabsorption when the culture is grown in a medium containing only lactose to induce a higher β-galactosidase level and increase rate of lactose uptake.