Protective effects of crude chalaza hydrolysates against liver fibrogenesis via antioxidation, anti-inflammation/anti-fibrogenesis, and apoptosis promotion of damaged hepatocytes

• Published in: Poultry science Vol. 100; no. 8; p. 101175
• Main Authors: Lin, Yi-Ling, Lu, Ching-Fu, Wu, Yi-Hsieng Samuel, Yang, Kuo-Tai, Yang, Wen-Yuan, Chen, Jr-Wei, Tseng, Jung-Kai, Chen, Yi-Chen
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.08.2021; Elsevier
• Subjects: anti-inflammation; antioxidant; apoptosis; crude chalaza hydrolysates; liver fibrosis; PROCESSING AND PRODUCT; crude chalaza hydrolysates; apoptosis; antioxidant; liver fibrosis; anti-inflammation
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2021.101175

Four-hundred metric-ton chalazae are produced annually from the liquid-egg processing and always cause a heavy burden due to handling cost in Taiwan. After chalazae were hydrolyzed by protease A, the amounts of hydrophobic, aromatic, and branched-chain amino acids, as well as anserine were dramatically increased. This study was to understand the antifibrogenic effects of protease A-digested crude chalaza hydrolysates (CCH-As) on livers of thioacetamide (TAA) treated rats. CCH-As improved (P< 0.05) growth performance, serum liver damage indices, histopathological liver inflammation, and liver collagen deposition in TAA-treated rats. The antifibrogenic effects of CCH-As were due to decreased (P < 0.05) inflammatory/fibrogenic cytokine contents, α-smooth-muscle-actin (α-SMA) protein expression, and matrix metallopeptidase (MMP)-2 and -9 activities, as well as increased (P < 0.05) the antioxidant capacity in livers. CCH-As also increased (P < 0.05) cleaved caspase-3 and cleaved poly ADP-ribose polymerase protein levels in livers of TAA-treated rats which accelerating cell renewal. Thus, this study does not only reveal a novel nutraceutical ingredient, CCH-As, against liver fibrogenesis, but also offer an alternative way to expand the utilization of poultry byproducts.