Automated Detection of Inaccurate and Imprecise Transitions in Peptide Quantification by Multiple Reaction Monitoring Mass Spectrometry

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 56; no. 2; pp. 291 - 305
• Main Authors: Abbatiello, Susan E, Mani, D. R, Keshishian, Hasmik, Carr, Steven A
• Format: Journal Article
• Language: English
• Published: Washington, DC Am Assoc Clin Chem 01.02.2010; American Association for Clinical Chemistry; Oxford University Press
• Subjects: Algorithms; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cancer; Chromatography; Data processing; Fundamental and applied biological sciences. Psychology; Humans; Investigative techniques, diagnostic techniques (general aspects); Mass spectrometry; Mass Spectrometry - methods; Medical research; Medical sciences; Molecular biophysics; Peptides; Peptides - blood; ROC Curve; Sensitivity and Specificity; Stable isotopes; Multiple; Peptides; Surveillance; Clinical biology; Quantization; Biochemistry; Diagnosis; Automatic analysis; Molecular biology; Detection; Mass spectrometry; Quantitative analysis
• ISSN: 0009-9147; 1530-8561; 1530-8561
• DOI: 10.1373/clinchem.2009.138420

Background: Multiple reaction monitoring mass spectrometry (MRM-MS) of peptides with stable isotope–labeled internal standards (SISs) is increasingly being used to develop quantitative assays for proteins in complex biological matrices. These assays can be highly precise and quantitative, but the fr...