Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii

• Published in: FEMS yeast research Vol. 18; no. 7; pp. 1 - 9
• Main Authors: Ito, Yoichiro, Watanabe, Toru, Aikawa, Shimpei, Nishi, Teruyuki, Nishiyama, Tozo, Nakamura, Yasuyuki, Hasunuma, Tomohisa, Okubo, Yuji, Ishii, Jun, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.11.2018
• Subjects: Analysis; Biotechnology; Deoxyribonucleic acid; DNA; DNA ligase (ATP); DNA Ligase ATP - genetics; DNA Ligase ATP - metabolism; DNA repair; Efficiency; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Deletion; Gene targeting; Gene Targeting - methods; genes; Genetic research; Homologous Recombination; Komagataella pastoris; Komagataella phaffii; Ku Autoantigen - genetics; Ku Autoantigen - metabolism; Ligases; Non-homologous end joining; protein synthesis; Recombinant proteins; Saccharomycetales - genetics; Species; telomeres; yeasts; Japan

The non-conventional yeast Komagataella phaffii, formerly Pichia pastoris, is a popular host for recombinant protein production. The relatively lower gene targeting efficiency observed in this species occurs due to high levels of non-homologous recombination activity. In the current study, we explor...