Modulation of Higher Order Chromatin Conformation in Mammalian Cell Nuclei Can Be Mediated by Polyamines and Divalent Cations

• Published in: PloS one Vol. 8; no. 6; p. e67689
• Main Authors: Visvanathan, Ashwat, Ahmed, Kashif, Even-Faitelson, Liron, Lleres, David, Bazett-Jones, David P., Lamond, Angus I.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.06.2013; Public Library of Science (PLoS)
• Subjects: Adenosine Triphosphate - metabolism; ATP; Biochemistry; Biochemistry, Molecular Biology; Biology; Calcium; Cancer; Cations; Cations, Divalent - metabolism; Cell Nucleus - metabolism; Cellular Biology; Chromatin; Chromatin - chemistry; Compaction; Condensation; Conformation; Deoxyglucose; Deoxyribonucleic acid; Depletion; Development Biology; Divalent cations; DNA; Fluorescence; Fluorescence microscopy; Fluorescence Resonance Energy Transfer; Genomes; HeLa Cells; Humans; Interphase; Life Sciences; Magnesium; Mammals; Microscopy; Microscopy, Fluorescence; Mitosis; Nuclei; Nuclei (cytology); Nucleosomes - metabolism; Polyamines; Polyamines - metabolism; Proteins; Sodium; Sodium azide; Spermidine; Spermine; Tomography; Transcription

The organisation of the large volume of mammalian genomic DNA within cell nuclei requires mechanisms to regulate chromatin compaction involving the reversible formation of higher order structures. The compaction state of chromatin varies between interphase and mitosis and is also subject to rapid an...