Comparison of DNA and RNA, and Cultivation Approaches for the Recovery of Terrestrial and Aquatic Fungi from Environmental Samples

• Published in: Current microbiology Vol. 66; no. 2; pp. 185 - 191
• Main Authors: Rao, Subramanya, Hyde, Kevin D, Pointing, Stephen B
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.02.2013; Springer Nature B.V
• Subjects: Anguillospora; Aquatic fungi; Ascomycota; Basidiomycota; Biodiversity; Biomedical and Life Sciences; Biotechnology; classification; Cultivation; Deoxyribonucleic acid; DNA; DNA libraries; DNA, Fungal; DNA, Fungal - genetics; Environmental Microbiology; forests; freshwater; Fungi; Fungi - classification; Fungi - isolation & purification; genetics; isolation & purification; Life Sciences; Metagenomics; Metagenomics - methods; methods; Microbiological Techniques; Microbiological Techniques - methods; Microbiology; Molecular Sequence Data; phylogeny; Ribonucleic acid; RNA; RNA libraries; RNA, Fungal; RNA, Fungal - genetics; Sequence Analysis, DNA; soil; Terminal Restriction Fragment Length Polymorphism; Trichoderma; Clone Library; Fungal Community; Internal Transcribe Spacer

Estimates of fungal biodiversity from environmental samples are all subject to bias. Major issues are that the commonly adopted cultivation-based approaches are suitable for taxa which grow readily under laboratory conditions, while the DNA-based approaches provide more reliable estimates, but do no...