Relevance of interferon-gamma in pathogenesis of life-threatening rapidly progressive interstitial lung disease in patients with dermatomyositis

• Published in: Arthritis research & therapy Vol. 20; no. 1; pp. 240 - 10
• Main Authors: Ishikawa, Yuichi, Iwata, Shigeru, Hanami, Kentaro, Nawata, Aya, Zhang, Mingzeng, Yamagata, Kaoru, Hirata, Shintaro, Sakata, Kei, Todoroki, Yasuyuki, Nakano, Kazuhisa, Nakayamada, Shingo, Satoh, Minoru, Tanaka, Yoshiya
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 26.10.2018; BioMed Central; BMC
• Subjects: Aged; Aged, 80 and over; Arthritis; Biomarkers; Biomarkers - blood; Care and treatment; Chemokines; Complications and side effects; Cytokines; Dermatomyositis; Dermatomyositis - blood; Dermatomyositis - diagnostic imaging; Dermatomyositis - epidemiology; Development and progression; Disease Progression; Female; Genetic aspects; Humans; Hypoxemia; IFN-γ; Immunoglobulins; Intensive care; Interferon; Interferon-gamma - blood; Interstitial lung diseases; Lung diseases; Lung Diseases, Interstitial - blood; Lung Diseases, Interstitial - diagnostic imaging; Lung Diseases, Interstitial - epidemiology; Lungs; Lymphatic system; Male; Medical prognosis; Middle Aged; Mortality; Pathogenesis; Patients; Rapidly progressive interstitial lung disease; Rheumatology; Risk factors; Sepsis; Severity of Illness Index; Spleen; Time Factors; Tomography; Tomography, X-Ray Computed - methods; Tumor necrosis factor-TNF; Japan; IFN-γ; Rapidly progressive interstitial lung disease; Dermatomyositis
• ISSN: 1478-6362; 1478-6354; 1478-6362
• DOI: 10.1186/s13075-018-1737-2

Dermatomyositis (DM) with rapidly progressive interstitial lung disease (DM RP-ILD) is a life-threatening condition. Serum cytokine levels are potentially suitable biomarkers for DM RP-ILD. However, the relationships among cytokine levels, lung imaging findings, and lung pathology have not been investigated. The aim of the present retrospective study was to determine the association between hypercytokinemia and lung inflammation in patients with DM RP-ILD. The study subjects were nine patients with life-threatening DM RP-ILD and severe hypoxemia (partial arterial oxygen pressure (PaO )/fraction of inspired oxygen (FiO ) ratio ≤ 200) before receiving intensive care management, who were admitted to our hospital between 2006 and 2015. The controls included 10 patients with DM without RP-ILD and 19 healthy subjects. We assessed the association between serum cytokine levels and computed tomography (CT) scores of the lung (ground glass opacity-score, G-score; fibrosis-score, F-score). Lung, hilar lymph nodes, and spleen from two autopsies were examined by hematoxylin-eosin (H&E) staining and immunostaining. Serum interferon (IFN)-γ, interleukin (IL)-1β and IL-12 levels were significantly higher in patients with DM RP-ILD than in the other two groups, whereas serum IL-6 levels were elevated in the two patient groups but not in the healthy subjects. Serum levels of IL-2, IL-4, IL-8, IL-10, IFN-α, and TNF (tumor necrosis factor)-α were not characteristically elevated in the DM RP-ILD group. Serum IFN-γ levels correlated with G-scores in patients with DM RP-ILD, while IL-1β was negatively correlation with F-scores. Immunohistochemical staining showed infiltration of numerous IFN-γ-positive histiocytes in the lung and hilar lymph nodes; but not in the spleen. Serum IL-6 levels did not correlate with the CT scores. Numerous IL-6-positive plasma cells were found in hilar lymph nodes, but not in the lungs or spleen. Our results suggest strong IFN-γ-related immune reaction in the lungs and hilar lymph nodes of patients with life-threatening DM RP-ILD, and potential IFN-γ involvement in the pathogenesis of DM, specifically in the pulmonary lesions of RP-ILD.