New Insights From Single-Cell Sequencing Data: Synovial Fibroblasts and Synovial Macrophages in Rheumatoid Arthritis

• Published in: Frontiers in Immunology Vol. 12; p. 709178
• Main Authors: Cheng, Liyun, Wang, Yanyan, Wu, Ruihe, Ding, Tingting, Xue, Hongwei, Gao, Chong, Li, Xiaofeng, Wang, Caihong
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media SA 19.07.2021; Frontiers Media S.A
• Subjects: Arthritis, Rheumatoid; Arthritis, Rheumatoid - pathology; biomarkers; Fibroblasts; Fibroblasts - classification; Fibroblasts - physiology; Humans; Immunologic diseases. Allergy; Immunology; Macrophages; Macrophages - physiology; RC581-607; rheumatoid arthritis; Sequence Analysis, RNA; Sequence Analysis, RNA - methods; Single-Cell Analysis; Single-Cell Analysis - methods; single-cell RNA sequencing; synovial fibroblast; synovial macrophage; Synovial Membrane; Synovial Membrane - cytology; single-cell RNA sequencing; synovial macrophage; synovial fibroblast; rheumatoid arthritis; biomarkers
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2021.709178

Single-cell RNA sequencing (scRNA-seq) technology can analyze the transcriptome expression level of cells with high-throughput from the single cell level, fully show the heterogeneity of cells, and provide a new way for the study of multicellular biological heterogeneity. Synovitis is the pathological basis of rheumatoid arthritis (RA). Synovial fibroblasts (SFs) and synovial macrophages are the core target cells of RA, which results in the destruction of articular cartilage, as well as bone. Recent scRNA-seq technology has made breakthroughs in the differentiation and development of two types of synovial cells, identification of subsets, functional analysis, and new therapeutic targets, which will bring remarkable changes in RA treatment.