Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 4; p. 989
• Main Authors: Choi, Hyeon Jun, Song, Bo Ram, Kim, Ji Eun, Bae, Su Ji, Choi, Yun Ju, Lee, Su Jin, Gong, Jeong Eun, Lee, Hee Seob, Lee, Chung Yeoul, Kim, Bae-Hwan, Hwang, Dae Youn
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.02.2020; MDPI
• Subjects: Aging; alpha-Linolenic Acid - chemistry; alpha-Linolenic Acid - pharmacology; Animal cognition; Animal models; Animals; Antioxidants; Apoptosis; Asthma; Biomarkers; Cell cycle; Cell lines; Cell viability; cold-pressed perilla oil; Diabetes; epidermis; Erythema; Fatty acids; Fibroblasts; Fibroblasts - drug effects; Galactosidase; Hairless; Histopathology; Humans; Hydration; Interstitial collagenase; Kinases; Linoleic acid; Linoleic Acid - chemistry; Linoleic Acid - pharmacology; Linolenic acid; Matrix metalloproteinase; Melanin; Mice; Mice, Hairless; mRNA; Oil; Oleic acid; Oleic Acid - chemistry; Oleic Acid - pharmacology; Palmitic acid; Perilla frutescens; photoaging; Phytochemicals; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Oils - chemistry; Plant Oils - pharmacology; ros; Seeds; Skin; Skin - drug effects; Skin - pathology; Skin - radiation effects; Skin Aging - drug effects; Skin Aging - pathology; Skin Aging - radiation effects; Stearic acid; Topical application; Ultraviolet radiation; Ultraviolet Rays - adverse effects; Water loss; wrinkle formation; UV; cold-pressed perilla oil; wrinkle formation; ROS; epidermis; photoaging
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25040989

Positive physiological benefits of several plant oils on the UV-induced photoaging have been reported in some cell lines and model mice, but perilla oil collected from the seeds of has not been investigated in this context. To study the therapeutic effects of cold-pressed perilla oil (CPO) on UV-induced photoaging in vitro and in vivo, UV-induced cellular damage and cutaneous photoaging were assessed in normal human dermal fibroblasts (NHDFs) and HR-1 hairless mice. CPO contained five major fatty acids including linolenic acid (64.11%), oleic acid (16.34%), linoleic acid (11.87%), palmitic acid (5.06%), and stearic acid (2.48%). UV-induced reductions in NHDF cell viability, ROS production, SOD activity, and G2/M cell cycle arrest were remarkably improved in UV + CPO treated NHDF cells as compared with UV + Vehicle treated controls. Also, UV-induced increases in MMP-1 protein and galactosidase levels were remarkably suppressed by CPO. In UV-radiated hairless mice, topical application of CPO inhibited an increase in wrinkle formation, transepidermal water loss (TEWL), erythema value, hydration and melanin index on dorsal skin of UVB-irradiated hairless mice. CPO was observed to similarly suppress UV-induced increases in epidermal thickness, mast cell numbers, and galactosidase and MMP-3 mRNA levels. These results suggest CPO has therapeutic potential in terms of protecting against skin photoaging by regulating skin morphology, histopathology and oxidative status.