Gram Stain-Specific-Probe-Based Real-Time PCR for Diagnosis and Discrimination of Bacterial Neonatal Sepsis

• Published in: Journal of Clinical Microbiology Vol. 46; no. 8; pp. 2613 - 2619
• Main Authors: Wu, Yi-Dong, Chen, Li-Hua, Wu, Xiu-Jing, Shang, Shi-Qiang, Lou, Jin-Tu, Du, Li-Zhong, Zhao, Zheng-Yan
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.2008; American Society for Microbiology (ASM)
• Subjects: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacterial diseases; Bacterial sepsis; Bacteriology; Biological and medical sciences; Blood - microbiology; DNA Primers - genetics; DNA, Bacterial - genetics; Female; Fundamental and applied biological sciences. Psychology; Human bacterial diseases; Humans; Infant, Newborn; Infant, Newborn, Diseases - diagnosis; Infant, Newborn, Diseases - microbiology; Infectious diseases; Male; Medical sciences; Microbiology; Miscellaneous; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Sepsis - diagnosis; Sepsis - microbiology; Human; Infection; Polymerase chain reaction; Sepsis syndrome; Newborn; Microbiology; Bacteriosis; Bacteria; Diagnosis; Real time; Bacteremia

Sepsis is a serious disease with high mortality in newborns. It is very important to have a convenient and accurate method for pathogenic diagnosis of neonatal sepsis. We developed a method of simultaneous detection and Gram classification of clinically relevant bacterial pathogens causing sepsis di...