Crystal structure of Spot 14, a modulator of fatty acid synthesis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 107; no. 44; pp. 18820 - 18825
• Main Authors: Colbert, Christopher L., Kim, Chai-Wan, Moon, Young-Ah, Henry, Lisa, Palnitkar, Maya, McKean, William B., Fitzgerald, Kevin, Deisenhofer, Johann, Horton, Jay D., Kwon, Hyock Joo
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 02.11.2010; National Acad Sciences
• Subjects: Acetyl-CoA Carboxylase - genetics; Acetyl-CoA Carboxylase - metabolism; Animals; Antibodies; BASIC BIOLOGICAL SCIENCES; Biochemistry; Biological Sciences; CARBOXYLASE; CARBOXYLIC ACIDS; Cellular immunity; CHO Cells; Cricetinae; Cricetulus; CRYSTAL STRUCTURE; Crystallography, X-Ray; Dimers; ENZYMES; Fatty acids; Fatty Acids - biosynthesis; Fatty Acids - chemistry; Fatty Acids - genetics; Female; Gene expression; GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; Hepatocytes; LIPIDS; Liver; MICE; Microtubule-Associated Proteins - chemistry; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Models, Molecular; Monomers; Nuclear Proteins - chemistry; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; POLYMERIZATION; Protein Structure, Tertiary; Protein synthesis; PROTEINS; Small interfering RNA; Structure-Activity Relationship; SYNTHESIS; Tissues; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors - metabolism

Spot 14 (S14) is a protein that is abundantly expressed in lipogenic tissues and is regulated in a manner similar to other enzymes involved in fatty acid synthesis. Deletion of S14 in mice decreased lipid synthesis in lactating mammary tissue, but the mechanism of S14’s action is unknown. Here we pr...