Determination of Five Coumarins in Angelicae Pubescentis Radix from Different Origins by HPTLC-Scanning

The HPTLC method is widely used in the field of quality evaluation and component analysis of traditional Chinese medicine (TCM). This work developed an HPTLC method to determine the five effective components of osthole, columbianadin, isoimperatorin, oxypeucedanin, and imperatorin in Angelicae Pubes...

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Published inJournal of analytical methods in chemistry Vol. 2022; pp. 1 - 7
Main Authors Ji, Dangtong, Li, Qian, Yang, Hanting, Fan, Yue, Wang, Ting, Chen, Yuying
Format Journal Article
LanguageEnglish
Published New York Hindawi 29.08.2022
John Wiley & Sons, Inc
Hindawi Limited
Subjects
Cluster analysis
Coumarins
Factor analysis
Herbal medicine
Linearity
Origins
Quality assessment
Quality control
Quantitative analysis
Regression analysis
Sample size
Stability analysis
Stability tests
Traditional Chinese medicine
China
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Abstract The HPTLC method is widely used in the field of quality evaluation and component analysis of traditional Chinese medicine (TCM). This work developed an HPTLC method to determine the five effective components of osthole, columbianadin, isoimperatorin, oxypeucedanin, and imperatorin in Angelicae Pubescentis Radix (APR) from twelve different origins, and the quality difference was analyzed by comprehensive factor analysis and cluster analysis. The results showed that the calibration curves of five components exhibited good linearity within the linear ranges (0.8–4.0 μg). The RSD of precision was 1.06%–1.21%, and the repeatability and stability tests were good. The results of cluster analysis showed that the APR from 12 different areas was divided into two categories, and at the same time, it was found that the quality of Dazhou in Sichuan and Huating in Gansu was better than in other areas. In this study, a simple, rapid, and efficient method for quality evaluation of TCM was established by the HPTLC method.
AbstractList The HPTLC method is widely used in the field of quality evaluation and component analysis of traditional Chinese medicine (TCM). This work developed an HPTLC method to determine the five effective components of osthole, columbianadin, isoimperatorin, oxypeucedanin, and imperatorin in Angelicae Pubescentis Radix (APR) from twelve different origins, and the quality difference was analyzed by comprehensive factor analysis and cluster analysis. The results showed that the calibration curves of five components exhibited good linearity within the linear ranges (0.8–4.0  μ g). The RSD of precision was 1.06%–1.21%, and the repeatability and stability tests were good. The results of cluster analysis showed that the APR from 12 different areas was divided into two categories, and at the same time, it was found that the quality of Dazhou in Sichuan and Huating in Gansu was better than in other areas. In this study, a simple, rapid, and efficient method for quality evaluation of TCM was established by the HPTLC method.
The HPTLC method is widely used in the field of quality evaluation and component analysis of traditional Chinese medicine (TCM). This work developed an HPTLC method to determine the five effective components of osthole, columbianadin, isoimperatorin, oxypeucedanin, and imperatorin in Angelicae Pubescentis Radix (APR) from twelve different origins, and the quality difference was analyzed by comprehensive factor analysis and cluster analysis. The results showed that the calibration curves of five components exhibited good linearity within the linear ranges (0.8–4.0 μg). The RSD of precision was 1.06%–1.21%, and the repeatability and stability tests were good. The results of cluster analysis showed that the APR from 12 different areas was divided into two categories, and at the same time, it was found that the quality of Dazhou in Sichuan and Huating in Gansu was better than in other areas. In this study, a simple, rapid, and efficient method for quality evaluation of TCM was established by the HPTLC method.
The HPTLC method is widely used in the field of quality evaluation and component analysis of traditional Chinese medicine (TCM). This work developed an HPTLC method to determine the five effective components of osthole, columbianadin, isoimperatorin, oxypeucedanin, and imperatorin in Angelicae Pubescentis Radix (APR) from twelve different origins, and the quality difference was analyzed by comprehensive factor analysis and cluster analysis. The results showed that the calibration curves of five components exhibited good linearity within the linear ranges (0.8-4.0μ g). The RSD of precision was 1.06%-1.21%, and the repeatability and stability tests were good. The results of cluster analysis showed that the APR from 12 different areas was divided into two categories, and at the same time, it was found that the quality of Dazhou in Sichuan and Huating in Gansu was better than in other areas. In this study, a simple, rapid, and efficient method for quality evaluation of TCM was established by the HPTLC method.
Audience Academic
Author Li, Qian
Ji, Dangtong
Wang, Ting
Yang, Hanting
Chen, Yuying
Fan, Yue
AuthorAffiliation State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
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CitedBy_id crossref_primary_10_1016_j_prmcm_2022_100212
crossref_primary_10_1016_j_chroma_2023_464564
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ContentType Journal Article
Copyright Copyright © 2022 Dangtong Ji et al.
COPYRIGHT 2022 John Wiley & Sons, Inc.
Copyright © 2022 Dangtong Ji et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0
Copyright © 2022 Dangtong Ji et al. 2022
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SubjectTerms Cluster analysis
Coumarins
Factor analysis
Herbal medicine
Linearity
Origins
Quality assessment
Quality control
Quantitative analysis
Regression analysis
Sample size
Stability analysis
Stability tests
Traditional Chinese medicine
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Title Determination of Five Coumarins in Angelicae Pubescentis Radix from Different Origins by HPTLC-Scanning
URI https://dx.doi.org/10.1155/2022/3415938
https://www.proquest.com/docview/2712664994
https://search.proquest.com/docview/2711840907
https://pubmed.ncbi.nlm.nih.gov/PMC9444472
https://doaj.org/article/eff1ba8ce75747b0b62d846626d04fd5
Volume 2022
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