Functional Reconstitution of the Purified Sodium Channel Protein from Rat Sarcolemma

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 79; no. 11; pp. 3651 - 3655
• Main Authors: Weigele, J. B., Barchi, R. L.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.06.1982; National Acad Sciences
• Subjects: Animals; Binding sites; Biological Transport; Eggs; Ethanol; Freeze Fracturing; Hot Temperature; Ion Channels - drug effects; Ion Channels - physiology; Liposomes; Muscle Proteins - isolation & purification; Phospholipids; Rats; Receptors; reconstitution; Sarcolemma; Sarcolemma - analysis; Saxitoxin - pharmacology; saxitoxin-binding protein; Sodium; Sodium - physiology; Sodium channels; Solubilization; Toxins; vesicles

The purified saxitoxin (STX) binding component of the rat sarcolemmal sodium channel (SBC) has been reconstituted into phospholipid vesicles. The reconstituted SBC displays the pharmacological properties and the ability to control sodium fluxes expected of a functional sodium channel. Batrachotoxin...