Chloroquine treatment induces secretion of autophagy-related proteins and inclusion of Atg8-family proteins in distinct extracellular vesicle populations

• Published in: Autophagy Vol. 18; no. 11; pp. 2547 - 2560
• Main Authors: Xu, Jing, Yang, Kevin C, Go, Nancy Erro, Colborne, Shane, Ho, Cally J, Hosseini-Beheshti, Elham, Lystad, Alf H, Simonsen, Anne, Guns, Emma Tomlinson, Morin, Gregg B, Gorski, Sharon M
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 02.11.2022; Landes Bioscience
• Subjects: Apoptosis Regulatory Proteins - metabolism; ATG16L1; Atg8; autophagy; Autophagy - physiology; Autophagy-Related Protein 8 Family - metabolism; Autophagy-Related Proteins - metabolism; CD63; chloroquine; Chloroquine - pharmacology; endosome; extracellular vesicle; Extracellular Vesicles - metabolism; gamma-Aminobutyric Acid; lysosome; MAP1LC3B; Research Paper; SDCBP/syntenin-1; Syntenins - metabolism; chloroquine; CD63; MAP1LC3B; endosome; autophagy; ATG16L1; SDCBP/syntenin-1; Atg8; extracellular vesicle; lysosome

Chloroquine (CQ), a lysosomotropic agent, is commonly used to inhibit lysosomal degradation and macroautophagy/autophagy. Here we investigated the cell-extrinsic effects of CQ on secretion. We showed that lysosomal and autophagy inhibition by CQ altered the secretome, and induced the release of Atg8...