Next-generation sequencing of representational difference analysis products for identification of genes involved in diosgenin biosynthesis in fenugreek (Trigonella foenum-graecum)

• Published in: Planta Vol. 245; no. 5; pp. 977 - 991
• Main Authors: Ciura, Joanna, Szeliga, Magdalena, Grzesik, Michalina, Tyrka, Mirosław
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Science + Business Media 01.05.2017; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Acetates - metabolism; Agriculture; biochemical pathways; Biomedical and Life Sciences; Biosynthesis; Cholesterol; complementary DNA; crops; Cyclopentanes - metabolism; diosgenin; Diosgenin - metabolism; DNA, Complementary - genetics; Ecology; Fabaceae; fenugreek; Forestry; High-Throughput Nucleotide Sequencing; Life Sciences; methyl jasmonate; ontogeny; Original; ORIGINAL ARTICLE; Oxylipins - metabolism; Phytosterols - metabolism; Plant Growth Regulators - metabolism; Plant Sciences; reverse transcriptase polymerase chain reaction; sapogenins; Sequence Analysis, DNA; squalene; Transcriptome; Trigonella - genetics; Trigonella - metabolism; Trigonella foenum-graecum; unigenes; Representational difference analysis of cDNA; Steroidal saponins; Diosgenin; Transcriptome user-friendly analysis; Next-generation sequencing; Phytosterols

Fenugreek (Trigonella foenum-graecum L.) is a valuable medicinal and crop plant. It belongs to Fabaceae family and has a unique potential to synthesize valuable steroidal saponins, e.g., diosgenin. Elicitation (methyl jasmonate) and precursor feeding (cholesterol and squalene) were used to enhance t...