Development of a PCR system for porcine cytomegalovirus detection and determination of the putative partial sequence of its DNA polymerase gene
After PCR amplification with conservative cytomegalovirus primers, a 520 nucleotide putative partial sequence of the DNA polymerase gene of porcine cytomegalovirus (PCMV) was determined. Sequence comparison revealed homology to DNA polymerase genes from various beta herpes viruses and a dendrogram w...
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Published in | Epidemiology and infection Vol. 123; no. 1; pp. 177 - 180 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge
Cambridge University Press
01.08.1999
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Abstract | After PCR amplification with conservative cytomegalovirus primers, a 520 nucleotide putative
partial sequence of the DNA polymerase gene of porcine cytomegalovirus (PCMV) was
determined. Sequence comparison revealed homology to DNA polymerase genes from various
beta herpes viruses and a dendrogram was constructed depicting the relationship of PCMV to
other members of the Herpesviridae family. The dendrogram indicates that PCMV is indeed a
beta herpes virus that is more closely related to human herpes virus types 6 and 7 than to
type 5. To address the difficulties encountered during conventional PCMV detection and
characterization a set of nested PCR primers were constructed which generated DNA
fragments of 415 and 257 bp from the DNA polymerase gene. The nested PCR system proved
specific for PCMV and provided a novel means for the detection of this poorly characterized
herpes virus in pig populations, vaccines and in organs used in xenotransplantation. |
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AbstractList | After PCR amplification with conservative cytomegalovirus primers, a 520 nucleotide putative partial sequence of the DNA polymerase gene of porcine cytomegalovirus (PCMV) was determined. Sequence comparison revealed homology to DNA polymerase genes from various beta herpes viruses and a dendrogram was constructed depicting the relationship of PCMV to other members of the Herpesviridae family. The dendrogram indicates that PCMV is indeed a beta herpes virus that is more closely related to human herpes virus types 6 and 7 than to type 5. To address the difficulties encountered during conventional PCMV detection and characterization a set of nested PCR primers were constructed which generated DNA fragments of 415 and 257 bp from the DNA polymerase gene. The nested PCR system proved specific for PCMV and provided a novel means for the detection of this poorly characterized herpes virus in pig populations, vaccines and in organs used in xenotransplantation. After PCR amplification with conservative cytomegalovirus primers, a 520 nucleotide putative partial sequence of the DNA polymerase gene of porcine cytomegalovirus (PCMV) was determined. Sequence comparison revealed homology to DNA polymerase genes from various beta herpes viruses and a dendrogram was constructed depicting the relationship of PCMV to other members of the Herpesviridae family. The dendrogram indicates that PCMV is indeed a beta herpes virus that is more closely related to human herpes virus types 6 and 7 than to type 5. To address the difficulties encountered during conventional PCMV detection and characterization a set of nested PCR primers were constructed which generated DNA fragments of 415 and 257 bp from the DNA polymerase gene. The nested PCR system proved specific for PCMV and provided a novel means for the detection of this poorly characterized herpes virus in pig populations, vaccines and in organs used in xenotransplantation. After PCR amplification with conservative cytomegalovirus primers, a 520 nucleotide putative partial sequence of the DNA polymerase gene of porcine cytomegalovirus (PCMV) was determined. Sequence comparison revealed homology to DNA polymerase genes from various beta herpes viruses and a dendrogram was constructed depicting the relationship of PCMV to other members of the Herpesviridae family. The dendrogram indicates that PCMV is indeed a beta herpes virus that is more closely related to human herpes virus types 6 and 7 than to type 5. To address the difficulties encountered during conventional PCMV detection and characterization a set of nested PCR primers were constructed which generated DNA fragments of 415 and 257 bp from the DNA polymerase gene. The nested PCR system proved specific for PCMV and provided a novel means for the detection of this poorly characterized herpes virus in pig populations, vaccines and in organs used in xenotransplantation. |
Author | BANKS, M. BELAK, S. LOWINGS, J. P. WIDEN, B. F. |
AuthorAffiliation | Virology Department, Central Veterinary Laboratory/Veterinary Laboratories Agency, New Haw, Addlestone, United Kingdom |
AuthorAffiliation_xml | – name: Virology Department, Central Veterinary Laboratory/Veterinary Laboratories Agency, New Haw, Addlestone, United Kingdom |
Author_xml | – sequence: 1 givenname: B. F. surname: WIDEN fullname: WIDEN, B. F. organization: Virology Department, Central Veterinary Laboratory/Veterinary Laboratories Agency, New Haw, Addlestone, KT15 3NB, United Kingdom – sequence: 2 givenname: J. P. surname: LOWINGS fullname: LOWINGS, J. P. organization: Virology Department, Central Veterinary Laboratory/Veterinary Laboratories Agency, New Haw, Addlestone, KT15 3NB, United Kingdom – sequence: 3 givenname: S. surname: BELAK fullname: BELAK, S. organization: Virology Department, Central Veterinary Laboratory/Veterinary Laboratories Agency, New Haw, Addlestone, KT15 3NB, United Kingdom – sequence: 4 givenname: M. surname: BANKS fullname: BANKS, M. organization: Virology Department, Central Veterinary Laboratory/Veterinary Laboratories Agency, New Haw, Addlestone, KT15 3NB, United Kingdom |
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Keywords | Porcine cytomegalovirus Nucleotide sequence Enzyme Herpesviridae Transferases Method Phylogeny Betaherpesvirinae Pig Infection Virus Polymerase chain reaction Nucleotidyltransferases Vertebrata Mammalia Animal Viral disease Artiodactyla Diagnosis Veterinary Detection Ungulata DNA-directed DNA polymerase |
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SubjectTerms | Animal viral diseases Animals Antibodies Base Sequence Biological and medical sciences Consensus Sequence Cytomegalovirus Cytomegalovirus - classification Cytomegalovirus - genetics Cytomegalovirus - isolation & purification Cytomegalovirus Infections - veterinary Cytomegalovirus Infections - virology DNA DNA Primers DNA, Viral - chemistry DNA-Directed DNA Polymerase - genetics Fundamental and applied biological sciences. Psychology Infections Infectious diseases Medical sciences Microbiology Molecular Sequence Data Nucleotide sequences Plasmids Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Porcine cytomegalovirus Sensitivity and Specificity SHORT PAPER Swine Swine Diseases - virology Techniques used in virology Viral diseases Virology Viruses |
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Title | Development of a PCR system for porcine cytomegalovirus detection and determination of the putative partial sequence of its DNA polymerase gene |
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