Headspace generation coupled to gas chromatography–mass spectrometry for the automated determination and quantification of endogenous compounds in urine. Aldehydes as possible markers of oxidative stress

• Published in: Journal of Chromatography A Vol. 1367; pp. 9 - 15
• Main Authors: Antón, Ana Pérez, Ferreira, Ana María Casas, Pinto, Carmelo García, Cordero, Bernardo Moreno, Pavón, José Luis Pérez
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07.11.2014; Elsevier
• Subjects: Adult; Aldehydes; Aldehydes - analysis; Aldehydes - urine; Analysis of variance; automation; Automation, Laboratory; Biological and medical sciences; Biomarkers - urine; Calibration; derivatization; experimental design; Gas chromatography; gas chromatography-mass spectrometry; Gas Chromatography-Mass Spectrometry - methods; headspace analysis; Headspace generation; Humans; Joining; Limit of Detection; Linearity; Mass spectrometry; Medical sciences; Methodology; Middle Aged; Oxidative Stress; Temperature; Tobacco, tobacco smoking; Toxicology; Urine; Young Adult; Urine; Oxidative stress; Aldehydes; Headspace generation; Biological fluid; Chemical analysis; Biological marker; Alkanal; Optimization; Smoker; Quantitative analysis; Human; Automation; Healthy subject; Coupled method; Benzaldehyde; Endogenous substance; Volatile organic compound; Aldehyde; Non smoker; Gas chromatography; Experimental design; Headspace; Mass spectrometry; Chemometrics

•HS-PTV-GC–MS coupling for determination of five aldehydes in urine samples.•Minimization of sample manipulation and shortening of pre-treatment time.•Simplification due to no derivatization requirement.•External calibration for quantification of analytes in healthy subjects’ urine.•LODs obtained ra...