miR-300 inhibits epithelial to mesenchymal transition and metastasis by targeting Twist in human epithelial cancer

• Published in: Molecular cancer Vol. 13; no. 1; p. 121
• Main Authors: Yu, Jingshuang, Xie, Furong, Bao, Xin, Chen, Wantao, Xu, Qin
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24.05.2014; BioMed Central
• Subjects: 3' Untranslated Regions; Analysis; Animals; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cell Line, Tumor; Epigenesis, Genetic; Epithelial Cells - metabolism; Epithelial Cells - pathology; Epithelial-Mesenchymal Transition - genetics; Female; Gene Expression Regulation, Neoplastic; Genes, Reporter; Genotype & phenotype; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - metabolism; Head and Neck Neoplasms - pathology; Humans; Luciferase; Luciferases - genetics; Luciferases - metabolism; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - secondary; Medical research; Metastasis; Mice; Mice, Nude; MicroRNAs - antagonists & inhibitors; MicroRNAs - genetics; MicroRNAs - metabolism; Microscopy; Neoplasm Invasiveness; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Physiological aspects; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Signal Transduction; Twist-Related Protein 1 - genetics; Twist-Related Protein 1 - metabolism; Xenograft Model Antitumor Assays

Epithelial-to-mesenchymal transition (EMT) is a key step of the progression of tumor cell metastasis. Recent work has demonstrated some miRNAs play critical roles in EMT. In this study, we focused on the roles of miR-300 in regulating EMT. The expression levels of miR-300 were examined in epithelial...