Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device

• Published in: Nature biotechnology Vol. 20; no. 8; pp. 826 - 830
• Main Authors: Toner, Mehmet, Li Jeon, Noo, Baskaran, Harihara, Dertinger, Stephan K. W, Whitesides, George M, Van De Water, Livingston
• Format: Journal Article
• Language: English
• Published: New York, NY Nature 01.08.2002; Nature Publishing Group
• Subjects: Biological and medical sciences; Biological Assay - instrumentation; Biological Assay - methods; Biotechnology; Cancer; Cell Culture Techniques - instrumentation; Cell physiology; Chemotactic Factors - pharmacology; Chemotaxis, Leukocyte - drug effects; Cliffs; Cytokines; Dose-Response Relationship, Drug; Equipment Design; Fundamental and applied biological sciences. Psychology; Interleukin-8 - pharmacology; Lithography; Medical research; Microchemistry; Miniaturization; Molecular and cellular biology; Motility and taxis; Neutrophils; Neutrophils - cytology; Neutrophils - drug effects; Reynolds number; Gradient; Neutrophil; Chemotaxis; Interleukin 8; Cytokine; Microequipment

Although a wealth of knowledge about chemotaxis has accumulated in the past 40 years, these studies have been hampered by the inability of researchers to generate simple linear gradients instantaneously and to maintain them at steady state. Here we describe a device microfabricated by soft lithograp...