Stable S/MAR-based episomal vectors are regulated at the chromatin level

• Published in: Chromosome research Vol. 18; no. 7; pp. 757 - 775
• Main Authors: Tessadori, Federico, Zeng, Kang, Manders, Erik, Riool, Martijn, Jackson, Dean, van Driel, Roel
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.11.2010; Springer Netherlands; Springer Nature B.V
• Subjects: Acetylation; Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; Cell Biology; Cell Nucleus - metabolism; Cell Nucleus - ultrastructure; ChIP; CHO Cells; Chromatin; Chromatin - genetics; Chromatin - metabolism; Cricetinae; Cricetulus; Episome; gene therapy; Genes, Reporter; Genetic Vectors - genetics; Genetic Vectors - metabolism; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Herpes Simplex Virus Protein Vmw65 - genetics; Histones - metabolism; Human Genetics; In vivo; Interphase; Lac Operon; lacO/LacR; Life Sciences; mammals; Methylation; Mice; mutation; Plant Genetics and Genomics; Plasmids - genetics; Plasmids - metabolism; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - genetics; reporter genes; S/MAR; transcription (genetics); Transcriptional Activation; Vector space; lacO/LacR; Episome; In vivo; Chromatin; S/MAR; ChIP

Episomal vectors assembled from defined genetic components are a promising alternative to traditional gene therapy vectors that integrate in the host genome and may cause insertional mutations. The vector pEPI-eGFP is stably retained in the episomal state in cultured mammalian cells at low copy numb...