Nuclear receptor interaction protein, a coactivator of androgen receptors (AR), is regulated by AR and Sp1 to feed forward and activate its own gene expression through AR protein stability

• Published in: Nucleic acids research Vol. 36; no. 1; pp. 51 - 66
• Main Authors: Chen, Pei-Hong, Tsao, Yeou-Ping, Wang, Chih-Chiang, Chen, Show-Li
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2008; Oxford Publishing Limited (England)
• Subjects: Adaptor Proteins, Signal Transducing; Amino Acid Sequence; androgen receptors; Animals; Base Sequence; Binding Sites; Cell Line; Cell Line, Tumor; chromatin; DNA; gene expression; genes; Humans; luciferase; Male; Molecular Biology; Molecular Sequence Data; neoplasm cells; Nuclear Proteins - genetics; nucleotide sequences; precipitin tests; Promoter Regions, Genetic; Prostate cancer; prostatic neoplasms; Prostatic Neoplasms - genetics; proteasome endopeptidase complex; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; RNA, Messenger - metabolism; Sp1 Transcription Factor - metabolism; transcription (genetics); Transcriptional Activation; Up-Regulation

Previously, we found a novel gene, nuclear receptor interaction protein (NRIP), a transcription cofactor that can enhance an AR-driven PSA promoter activity in a ligand-dependent manner in prostate cancer cells. Here, we investigated NRIP regulation. We cloned a 413-bp fragment from the transcriptio...