Dicer is essential for formation of the heterochromatin structure in vertebrate cells

• Published in: Nature cell biology Vol. 6; no. 8; pp. 784 - 791
• Main Authors: Fukagawa, Tatsuo, Nogami, Masahiro, Yoshikawa, Mitsuko, Ikeno, Masashi, Okazaki, Tuneko, Takami, Yasunari, Nakayama, Tatsuo, Oshimura, Mitsuo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.08.2004; Nature Publishing Group
• Subjects: Accumulation; Animals; Biomedical and Life Sciences; Blotting, Western; Cancer Research; Cell Biology; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell Death - genetics; Cell Line; Cell Survival; Cells; Centromere - chemistry; Chickens; Chromatin; Chromosomes, Human, Pair 21; Developmental Biology; Endoribonucleases - deficiency; Endoribonucleases - genetics; Endoribonucleases - physiology; Gene Silencing; Genetic aspects; Heterochromatin - chemistry; Heterochromatin - genetics; Heterochromatin - metabolism; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; letter; Life Sciences; Models, Biological; Mutation; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Phosphoproteins - genetics; Phosphoproteins - metabolism; Physiological aspects; Protein Kinases - genetics; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases; Proteins; Restriction Mapping; RNA Interference; Stem Cells; Transgenes; Vertebrates; Japan

RNA interference is an evolutionarily conserved gene-silencing pathway in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs. The biological function of the RNAi-related pathway in vertebrate cells is not fully understood. Here, we report the generation of a conditional...