Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts

• Published in: Journal of experimental botany Vol. 61; no. 14; pp. 4043 - 4054
• Main Authors: Pulido, Pablo, Spínola, María Cristina, Kirchsteiger, Kerstin, Guinea, Manuel, Pascual, María Belén, Sahrawy, Mariam, Sandalio, Luisa María, Dietz, Karl-Josef, González, Maricruz, Cejudo, Francisco Javier
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.09.2010
• Subjects: Antioxidants; Antioxidants - metabolism; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Biological and medical sciences; Chloroplast; Chloroplasts; Chloroplasts - enzymology; Chloroplasts - metabolism; Cysteine - metabolism; Darkness; Enzymes; Fundamental and applied biological sciences. Psychology; Hydrogen Peroxide - metabolism; Leaves; Light; NADP - metabolism; Oxidation-Reduction; Oxidative stress; Oxidative Stress - physiology; Peroxides; peroxiredoxin; Peroxiredoxins - metabolism; Phenotypes; Photosynthesis; Plant physiology; Plants; Research Papers; Thioredoxin; Thioredoxin-Disulfide Reductase - metabolism; Thioredoxins - metabolism; Arabidopsis thaliana; Oxidative stress; peroxiredoxin; Cruciferae; Dicotyledones; Angiospermae; Botany; Spermatophyta; Functional analysis; Experimental plant; Thioredoxin; Chloroplast
• DOI: 10.1093/jxb/erq218
• ISSN: 0022-0957

Photosynthesis is a process that inevitably produces reactive oxygen species, such as hydrogen peroxide, which is reduced by chloroplast-localized detoxification mechanisms one of which involves 2-Cys peroxiredoxins (2-Cys Prxs). Arabidopsis chloroplasts contain two very similar 2-Cys Prxs (denoted A and B). These enzymes are reduced by two pathways: NADPH thioredoxin reductase C (NTRC), which uses NADPH as source of reducing power; and plastidial thioredoxins (Trxs) coupled to photosynthetically reduced ferredoxin of which Trx x is the most efficient reductant in vitro. With the aim of establishing the functional relationship between NTRC, Trx x, and 2-Cys Prxs in vivo, an Arabidopsis Trx x knock-out mutant has been identified and a double mutant (denoted Δ2cp) with <5% of 2-Cys Prx content has been generated. The phenotypes of the three mutants, ntrc, trxx, and Δ2cp, were compared under standard growth conditions and in response to continuous light or prolonged darkness and oxidative stress. Though all mutants showed altered redox homeostasis, no difference was observed in response to oxidative stress treatment. Moreover, the redox status of the 2-Cys Prx was imbalanced in the ntrc mutant but not in the trxx mutant. These results show that NTRC is the most relevant pathway for chloroplast 2-Cys Prx reduction in vivo, but the antioxidant function of this system is not essential. The deficiency of NTRC caused a more severe phenotype than the deficiency of Trx x or 2-Cys Prxs as determined by growth, pigment content, CO₂ fixation, and Fv/Fm, indicating additional functions of NTRC.