ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation

• Published in: Developmental cell Vol. 56; no. 4; pp. 427 - 442.e5
• Main Authors: Miao, Guangyan, Zhao, Hongyu, Li, Yan, Ji, Mingming, Chen, Yong, Shi, Yi, Bi, Yuhai, Wang, Peihui, Zhang, Hong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 22.02.2021
• Subjects: Autophagosomes - metabolism; Autophagy; Autophagy-Related Proteins - metabolism; COVID-19; COVID-19 - metabolism; COVID-19 - virology; DMV; HEK293 Cells; HeLa Cells; HOPS; Humans; Lysosomes - metabolism; ORF3a; Protein Binding; SARS-CoV-2; SARS-CoV-2 - metabolism; SARS-CoV-2 - pathogenicity; SNARE; SNARE Proteins - metabolism; Vesicular Transport Proteins - metabolism; Viroporin Proteins - genetics; Viroporin Proteins - metabolism; COVID-19; SARS-CoV-2; autophagy; HOPS; DMV; ORF3a; SNARE

Autophagy acts as a cellular surveillance mechanism to combat invading pathogens. Viruses have evolved various strategies to block autophagy and even subvert it for their replication and release. Here, we demonstrated that ORF3a of the COVID-19 virus SARS-CoV-2 inhibits autophagy activity by blockin...