Evaluation of sensitivity of TaqMan RT-PCR for rubella virus detection in clinical specimens

• Published in: Journal of clinical virology Vol. 80; pp. 98 - 101
• Main Authors: Okamoto, Kiyoko, Mori, Yoshio, Komagome, Rika, Nagano, Hideki, Miyoshi, Masahiro, Okano, Motohiko, Aoki, Yoko, Ogura, Atsushi, Hotta, Chiemi, Ogawa, Tomoko, Saikusa, Miwako, Kodama, Hiroe, Yasui, Yoshihiro, Minagawa, Hiroko, Kurata, Takako, Kanbayashi, Daiki, Kase, Tetsuo, Murata, Sachiko, Shirabe, Komei, Hamasaki, Mitsuhiro, Kato, Takashi, Otsuki, Noriyuki, Sakata, Masafumi, Komase, Katsuhiro, Takeda, Makoto
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2016
• Subjects: Allergy and Immunology; Female; Humans; Infectious Disease; Japan; Laboratory diagnosis; Male; Pharynx - virology; Real-Time Polymerase Chain Reaction - methods; RNA, Viral - genetics; Rubella; Rubella - diagnosis; Rubella virus; Rubella virus - genetics; Rubella virus - isolation & purification; Sensitivity and Specificity; TaqMan real-time RT-PCR; Urine - virology; TaqMan real-time RT-PCR; Laboratory diagnosis; Rubella

•We evaluated a TaqMan RT-PCR assay for the rubella surveillance system in Japan.•We tested its sensitivity with all rubella virus genotypes and clinical specimens.•Detection limits for the rubella virus genotypes ranged from 1 to 10 pfu/reaction.•The sensitivity of the TaqMan RT-PCR was lower than...