Identification of shared and unique immunoglobulin E epitopes of the highly conserved tropomyosins in Blomia tropicalis and Dermatophagoides pteronyssinus

• Published in: Clinical and experimental allergy Vol. 32; no. 8; pp. 1203 - 1210
• Main Authors: Yi, F. C., Cheong, N., Shek, P. C. L., Wang, D. Y., Chua, K. Y., Lee, B. W.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science, Ltd 01.08.2002; Blackwell; Wiley Subscription Services, Inc
• Subjects: Adolescent; Adult; Aged; allergen; Allergens - genetics; Allergens - immunology; Allergic diseases; Animals; Biological and medical sciences; Blomia tropicalis; Child; Child, Preschool; Cross Reactions; Dermatophagoides pteronyssinus; Dermatophagoides pteronyssinus - immunology; DNA Probes; Dust; Epitopes - analysis; Epitopes - genetics; Gene Library; General aspects; Humans; Hypersensitivity - immunology; IgE epitope; Immunoglobulin E - blood; Immunoglobulin E - immunology; Immunopathology; Medical sciences; Mice; Middle Aged; Mites - immunology; Sequence Analysis, DNA; Singapore; Skin Tests; tropomyosin; Tropomyosin - immunology; Singapore; Human; Immunopathology; Allergy; Prick test; Antigenic determinant; Tropomyosin; IgE; Acaridida; Dermatophagoides pteronyssinus; Polymerase chain reaction; Acariformes; Arachnida; Acari; Arthropoda; Cross reaction; Pyroglyphidae; Molecular biology; Invertebrata; ELISA assay; Allergen; Skin test

Summary Background Tropomyosin belongs to a class of highly conserved proteins in invertebrates and vertebrates. The invertebrate tropomyosins are allergenic in man with high IgE cross‐reactivity and have been therefore referred to as pan‐allergens. Objectives This study aimed to clone and identify...