Distal ExsA-Binding Site in Pseudomonas aeruginosa Type III Secretion System Promoters Is the Primary Determinant for Promoter-Specific Properties

• Published in: Journal of Bacteriology Vol. 194; no. 10; pp. 2564 - 2572
• Main Authors: Brutinel, Evan D, King, Jessica M, Marsden, Anne E, Yahr, Timothy L
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.05.2012
• Subjects: adenine; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Base Sequence; Binding Sites; Biological and medical sciences; Cells; DNA, Bacterial; DNA-binding domains; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial - physiology; Gram-negative bacteria; hybrids; Microbiology; Miscellaneous; Mutagenesis; Mutation; promoter regions; Promoter Regions, Genetic - genetics; Protein Binding; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Trans-Activators - genetics; Trans-Activators - metabolism; Tuberculosis; Type III secretion system; Pseudomonadales; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa; Binding site; Type III secretion system

Transcription of the Pseudomonas aeruginosa type III secretion system is controlled by ExsA, a member of the AraC/XylS family of regulators. Each ExsA-dependent promoter contains two adjacent binding sites for monomeric ExsA. The promoter-proximal site (binding site 1) consists of highly conserved G...