Histone methyltransferase G9a promotes liver cancer development by epigenetic silencing of tumor suppressor gene RARRES3

• Published in: Journal of hepatology Vol. 67; no. 4; pp. 758 - 769
• Main Authors: Wei, Lai, Chiu, David Kung-Chun, Tsang, Felice Ho-Ching, Law, Cheuk-Ting, Cheng, Carol Lai-Hung, Au, Sandy Leung-Kuen, Lee, Joyce Man-Fong, Wong, Carmen Chak-Lui, Ng, Irene Oi-Lin, Wong, Chun-Ming
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2017; Elsevier Science Ltd
• Subjects: 3' Untranslated Regions; Animal models; Animals; Carcinogenesis; Carcinogens; Carcinoma, Hepatocellular - enzymology; Carcinoma, Hepatocellular - etiology; Carcinoma, Hepatocellular - genetics; Cell Line, Tumor; Cell proliferation; Chromosome 6; Copy number; CRISPR; Enzymes; Epigenesis, Genetic; Epigenetics; G9a; Gastroenterology and Hepatology; Gene amplification; Gene Dosage; Gene expression; Gene Knockdown Techniques; Gene Knockout Techniques; Gene Silencing; Genes, Tumor Suppressor; Hepatocellular carcinoma; Histocompatibility Antigens - genetics; Histocompatibility Antigens - metabolism; Histone methyltransferase; Histone modifications; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - metabolism; Humans; Liver cancer; Liver Neoplasms - enzymology; Liver Neoplasms - etiology; Liver Neoplasms - genetics; Liver Neoplasms, Experimental - enzymology; Liver Neoplasms, Experimental - etiology; Liver Neoplasms, Experimental - genetics; Metastases; Mice; Mice, Nude; MicroRNAs - genetics; MicroRNAs - metabolism; miR-1; Post-transcription; RARRES3; Receptors, Retinoic Acid - antagonists & inhibitors; Receptors, Retinoic Acid - genetics; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Neoplasm - genetics; RNA, Neoplasm - metabolism; RNA-mediated interference; Tumor suppressor genes; Up-Regulation; Epigenetics; Gene amplification; Histone modifications; miR-1; RARRES3; G9a

[Display omitted] •G9a was frequently upregulated in human HCC and associated with HCC aggressiveness.•G9a promoted HCC growth and metastasis both in vitro and in vivo.•Upregulation of G9a in HCC was attributed to gene amplification and loss of miR-1.•G9a epigenetically silenced the expression of tu...