A key role for mitochondria in endothelial signaling by plasma cysteine/cystine redox potential

• Published in: Free radical biology & medicine Vol. 48; no. 2; pp. 275 - 283
• Main Authors: Go, Young-Mi, Park, Heonyong, Koval, Michael, Orr, Michael, Reed, Matthew, Liang, Yongliang, Smith, Debra, Pohl, Jan, Jones, Dean P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.01.2010
• Subjects: Actin; Animals; Cardiovascular Diseases - metabolism; Cardiovascular Diseases - pathology; Cardiovascular Diseases - physiopathology; Cell Adhesion - genetics; Cysteine - metabolism; Cystine - metabolism; Cytoskeletal Proteins - metabolism; Cytoskeleton; Endothelial cells; Endothelium, Vascular - metabolism; Endothelium, Vascular - pathology; Extracellular redox state; Free radicals; Humans; Mass Spectrometry; Membrane Proteins - metabolism; Mice; Mice, Transgenic; Mitochondria - genetics; Mitochondria - metabolism; Mitochondrial thioredoxin 2; NIH 3T3 Cells; Oxidation-Reduction; Oxidative Stress; Proinflammatory signaling; Proteomics; Reactive Oxygen Species - metabolism; Redox ICAT; Redox proteomics; Signal Transduction - genetics; Thioredoxins - genetics; Thioredoxins - metabolism; E hCySS; GSSG; Free radicals; DTT; DCF; AMS; Proinflammatory signaling; Redox ICAT; Endothelial cells; ICAT; CVD; MAEC; Redox proteomics; Tg; NLS; Extracellular redox state; Actin; CySS; ROS; Cytoskeleton; Mitochondrial thioredoxin 2; Trx; GSH; WT

The redox potential of the plasma cysteine/cystine couple ( E hCySS) is oxidized in association with risk factors for cardiovascular disease (CVD), including age, smoking, type 2 diabetes, obesity, and alcohol abuse. Previous in vitro findings support a cause–effect relationship for extracellular E...