Three dimensional multiphoton imaging of fresh and whole mount developing mouse mammary glands

• Published in: BMC cancer Vol. 13; no. 1; p. 373
• Main Authors: Johnson, Michael D, Mueller, Susette C
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 06.08.2013; BioMed Central
• Subjects: Animals; Breasts; Collagen; Cytomegalovirus; Diagnostic imaging; Dietary fiber; Extracellular Matrix - metabolism; Extracellular Matrix - pathology; Female; Fiber optic networks; Genetic engineering; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Health services; Imaging, Three-Dimensional; Mammary glands; Mammary Glands, Animal - growth & development; Mammary Glands, Animal - metabolism; Mammary Glands, Animal - pathology; Membrane Glycoproteins - physiology; Methods; Mice; Mice, Transgenic; Microscopy; Microscopy, Fluorescence, Multiphoton - methods; Public health; Rodents; Studies; Technical Advance; United States

The applications of multiphoton microscopy for deep tissue imaging in basic and clinical research are ever increasing, supplementing confocal imaging of the surface layers of cells in tissue. However, imaging living tissue is made difficult by the light scattering properties of the tissue, and this...