Real-Time Analysis of the Effects of Cholesterol on Lipid Raft Behavior Using Atomic Force Microscopy

• Published in: Biophysical journal Vol. 84; no. 3; pp. 1827 - 1832
• Main Authors: Lawrence, Jared C., Saslowsky, David E., Michael Edwardson, J., Henderson, Robert M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2003; Biophysical Society
• Subjects: Cellular biology; Cholesterol; Cholesterol - chemistry; Filipin - chemistry; Lipid Bilayers - chemistry; Lipids; Macromolecular Substances; Membrane Fluidity; Membrane Microdomains - chemistry; Membranes; Microscopy, Atomic Force - methods; Molecular Conformation; Phosphatidylcholines - chemistry; Sphingomyelins - chemistry; Time Factors

Cholesterol plays a crucial role in cell membranes, and has been implicated in the assembly and maintenance of sphingolipid-rich rafts. We have examined the cholesterol-dependence of model rafts (sphingomyelin-rich domains) in supported lipid monolayers and bilayers using atomic force microscopy. Sp...