The Impact of DNA Polymerase and Number of Rounds of Amplification in PCR on 16S rRNA Gene Sequence Data

• Published in: mSphere Vol. 4; no. 3
• Main Authors: Sze, Marc A., Schloss, Patrick D.
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 22.05.2019
• Subjects: 16S rRNA gene; Abundance; Bacteria - genetics; Bias; Data analysis; Deoxyribonucleic acid; DNA; DNA polymerase; DNA, Bacterial - genetics; DNA-directed DNA polymerase; DNA-Directed DNA Polymerase - genetics; DNA-Directed DNA Polymerase - standards; Ecological and Evolutionary Science; environmental microbiology; Feces - microbiology; Fidelity; Humans; Investigations; microbial ecology; microbiome; Microbiota; Nucleotide sequence; PCR; Pipelines; Polymerase chain reaction; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - standards; Researchers; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA - standards; Software; Taxonomy; environmental microbiology; polymerase; bias; sequence analysis; microbial ecology; microbiome; PCR; 16S rRNA gene

A steep decline in sequencing costs drove an explosion in studies characterizing microbial communities from diverse environments. Although a significant amount of effort has gone into understanding the error profiles of DNA sequencers, little has been done to understand the downstream effects of the...