Membrane potential dye imaging of ventromedial hypothalamus neurons from adult mice to study glucose sensing

• Published in: Journal of visualized experiments Vol. 81; no. 81; pp. 1 - 9
• Main Authors: Vazirani, Reema P, Fioramonti, Xavier, Routh, Vanessa H
• Format: Journal Article
• Language: English
• Published: United States JoVE 2013; MyJove Corporation
• Subjects: Animal biology; Animals; Development Biology; Fluorescent Dyes - chemistry; Glucose - metabolism; Life Sciences; Male; Membrane Potentials - physiology; Mice; Mice, Inbred C57BL; Neurons - cytology; Neurons - metabolism; Neurons - physiology; Neurons and Cognition; Neuroscience; Spectrometry, Fluorescence - methods; Staining and Labeling - methods; Ventromedial Hypothalamic Nucleus - cytology; Ventromedial Hypothalamic Nucleus - metabolism; Ventromedial Hypothalamic Nucleus - physiology; inbred c57bl; nucleus/cytology/metabolism/ physiology; glucose/ metabolism; fluorescence/ methods; neurons/cytology/metabolism/ physiology; ventromedial hypothalamic; fluorescent dyes/ chemistry; mice; staining and labeling/ methods; membrane potentials/physiology; spectrometry; animals; male

Studies of neuronal activity are often performed using neurons from rodents less than 2 months of age due to the technical difficulties associated with increasing connective tissue and decreased neuronal viability that occur with age. Here, we describe a methodology for the dissociation of healthy h...