Cross-Bridge Number, Position, and Angle in Target Zones of Cryofixed Isometrically Active Insect Flight Muscle

• Published in: Biophysical journal Vol. 86; no. 5; pp. 3009 - 3019
• Main Authors: Tregear, Richard T., Reedy, Mary C., Goldman, Yale E., Taylor, Kenneth A., Winkler, Hanspeter, Franzini-Armstrong, Clara, Sasaki, Hiroyuki, Lucaveche, Carmen, Reedy, Michael K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2004; Biophysical Society; The Biophysical Society
• Subjects: Actins - chemistry; Actins - metabolism; Adenosine Triphosphate - chemistry; Animals; Calcium - metabolism; Cellular biology; Flight, Animal; Hemiptera; Image Processing, Computer-Assisted; Kinetics; Microscopy, Electron; Muscle Contraction; Muscle Fibers, Skeletal - cytology; Muscles - metabolism; Muscles and Contractility; Muscular system; Myosin Subfragments - chemistry; Myosins - chemistry; Normal Distribution; Proteins

Electron micrographic tomograms of isometrically active insect flight muscle, freeze substituted after rapid freezing, show binding of single myosin heads at varying angles that is largely restricted to actin target zones every 38.7 nm. To quantify the parameters that govern this pattern, we measure...