Type 2 innate lymphoid cells disrupt bronchial epithelial barrier integrity by targeting tight junctions through IL-13 in asthmatic patients

• Published in: Journal of allergy and clinical immunology Vol. 141; no. 1; pp. 300 - 310.e11
• Main Authors: Sugita, Kazunari, Steer, Catherine A., Martinez-Gonzalez, Itziar, Altunbulakli, Can, Morita, Hideaki, Castro-Giner, Francesc, Kubo, Terufumi, Wawrzyniak, Paulina, Rückert, Beate, Sudo, Katsuko, Nakae, Susumu, Matsumoto, Kenji, O'Mahony, Liam, Akdis, Mübeccel, Takei, Fumio, Akdis, Cezmi A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2018; Elsevier Limited
• Subjects: Allergies; Allergy and Immunology; Animal models; Animals; Asthma; Asthma - immunology; Asthma - metabolism; barrier; Bone marrow transplantation; bronchial epithelium; Cytokines; Dermatitis; Dextran; Disease Models, Animal; Epithelial cells; Epithelial Cells - metabolism; Epithelium; Fluorescein isothiocyanate; Humans; IL-13; Immune system; Immunity, Innate; Inflammation; Innate lymphoid cell; Interleukin 13; Interleukin-13 - antagonists & inhibitors; Interleukin-13 - metabolism; Intranasal administration; Lymphocyte Subsets - immunology; Lymphocyte Subsets - metabolism; Lymphocytes B; Lymphoid cells; Medical research; Mice; Mice, Transgenic; mRNA; Mucus - metabolism; Pathogens; Permeability; Pollutants; RAG gene; RAG2 protein; Recombination; Respiratory Mucosa - immunology; Respiratory Mucosa - metabolism; Respiratory Mucosa - pathology; Respiratory tract; Respiratory tract diseases; tight junction; Tight junctions; Tight Junctions - metabolism; Transplantation; TER; EF1α; ILC; Innate lymphoid cell; BMT; GO; IL-13; BAL; HDM; ILC2; FITC; barrier; ROR; ZO; TJ; HBEC; Rag2; bronchial epithelium; WT; tight junction; ALI; HDAC; Fluorescein isothiocyanate; Histone deacetylase; Bone marrow transplantation; Gene ontology; Human bronchial epithelial cell; Bronchoalveolar lavage; Recombination-activating gene 2; Zonula occludens; Transepithelial electrical resistance; Retinoic acid–related orphan receptor; Air-liquid interface; House dust mite; Wild-type; Elongation factor 1α; Type 2 innate lymphoid cell
• ISSN: 0091-6749; 1097-6825; 1097-6825
• DOI: 10.1016/j.jaci.2017.02.038

Bronchial epithelial barrier leakiness and type 2 innate lymphoid cells (ILC2s) have been separately linked to asthma pathogenesis; however, the influence of ILC2s on the bronchial epithelial barrier has not been investigated previously. We investigated the role of ILC2s in the regulation of bronchi...