Characterization of GAPCenA, a GTPase activating protein for Rab6, part of which associates with the centrosome
The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification and characterization of a novel human Rab6‐interacting protein named human GAPCenA (for ‘GAP and centrosome‐associated’). Primary sequence ana...
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| Published in | The EMBO journal Vol. 18; no. 7; pp. 1772 - 1782 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Chichester, UK
John Wiley & Sons, Ltd
01.04.1999
Blackwell Publishing Ltd |
| Subjects | |
| Online Access | Get full text |
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| Abstract | The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification and characterization of a novel human Rab6‐interacting protein named human GAPCenA (for ‘GAP and centrosome‐associated’). Primary sequence analysis indicates that GAPCenA displays similarities, within a central 200 amino acids domain, to both the yeast Rab GTPase activating proteins (GAPs) and to the spindle checkpoint proteins Saccharomyces cerevisiae Bub2p and Schizosaccharomyces pombe Cdc16p. We demonstrate that GAPCenA is indeed a GAP, specifically active in vitro on Rab6 and, to a lesser extent, on Rab4 and Rab2 proteins. Immunofluorescence and cell fractionation experiments showed that GAPCenA is mainly cytosolic but that a minor pool is associated with the centrosome. Moreover, GAPCenA was found to form complexes with cytosolic γ‐tubulin and to play a role in microtubule nucleation. Therefore, GAPCenA may be involved in the coordination of microtubule and Golgi dynamics during the cell cycle. |
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| AbstractList | The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification and characterization of a novel human Rab6-interacting protein named human GAPCenA (for 'GAP and centrosome-associated'). Primary sequence analysis indicates that GAPCenA displays similarities, within a central 200 amino acids domain, to both the yeast Rab GTPase activating proteins (GAPs) and to the spindle checkpoint proteins Saccharomyces cerevisiae Bub2p and Schizosaccharomyces pombe Cdc16p. We demonstrate that GAPCenA is indeed a GAP, specifically active in vitro on Rab6 and, to a lesser extent, on Rab4 and Rab2 proteins. Immunofluorescence and cell fractionation experiments showed that GAPCenA is mainly cytosolic but that a minor pool is associated with the centrosome. Moreover, GAPCenA was found to form complexes with cytosolic gamma-tubulin and to play a role in microtubule nucleation. Therefore, GAPCenA may be involved in the coordination of microtubule and Golgi dynamics during the cell cycle. The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification and characterization of a novel human Rab6-interacting protein named human GAPCenA (for 'GAP and centrosome-associated'). Primary sequence analysis indicates that GAPCenA displays similarities, within a central 200 amino acids domain, to both the yeast Rab GTPase activating proteins (GAPs) and to the spindle checkpoint proteinsSaccharomyces cerevisiae Bub2p and Schizosaccharomyces pombe Cdc16p. We demonstrate that GAPCenA is indeed a GAP, specifically active in vitro on Rab6 and, to a lesser extent, on Rab4 and Rab2 proteins. Immunofluorescence and cell fractionation experiments showed that GAPCenA is mainly cytosolic but that a minor pool is associated with the centrosome. Moreover, GAPCenA was found to form complexes with cytosolic [gamma]-tubulin and to play a role in microtubule nucleation. Therefore, GAPCenA may be involved in the coordination of microtubule and Golgi dynamics during the cell cycle. The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification and characterization of a novel human Rab6‐interacting protein named human GAPCenA (for ‘GAP and centrosome‐associated’). Primary sequence analysis indicates that GAPCenA displays similarities, within a central 200 amino acids domain, to both the yeast Rab GTPase activating proteins (GAPs) and to the spindle checkpoint proteins Saccharomyces cerevisiae Bub2p and Schizosaccharomyces pombe Cdc16p. We demonstrate that GAPCenA is indeed a GAP, specifically active in vitro on Rab6 and, to a lesser extent, on Rab4 and Rab2 proteins. Immunofluorescence and cell fractionation experiments showed that GAPCenA is mainly cytosolic but that a minor pool is associated with the centrosome. Moreover, GAPCenA was found to form complexes with cytosolic γ‐tubulin and to play a role in microtubule nucleation. Therefore, GAPCenA may be involved in the coordination of microtubule and Golgi dynamics during the cell cycle. |
| Author | Bordes, Nicole Possmayer, Franziska Zander, Hilke Jollivet, Florence Cuif, Marie-Hélène Bornens, Michel Langsley, Gordon Goud, Bruno Couedel-Courteille, Anne Janoueix-Lerosey, Isabelle |
| AuthorAffiliation | UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France |
| AuthorAffiliation_xml | – name: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France |
| Author_xml | – sequence: 1 givenname: Marie-Hélène surname: Cuif fullname: Cuif, Marie-Hélène – sequence: 2 givenname: Franziska surname: Possmayer fullname: Possmayer, Franziska organization: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, Cedex 05, 75248, Paris, France – sequence: 3 givenname: Hilke surname: Zander fullname: Zander, Hilke organization: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, Cedex 05, 75248, Paris, France – sequence: 4 givenname: Nicole surname: Bordes fullname: Bordes, Nicole organization: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, Cedex 05, 75248, Paris, France – sequence: 5 givenname: Florence surname: Jollivet fullname: Jollivet, Florence organization: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, Cedex 05, 75248, Paris, France – sequence: 6 givenname: Anne surname: Couedel-Courteille fullname: Couedel-Courteille, Anne organization: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, Cedex 05, 75248, Paris, France – sequence: 7 givenname: Isabelle surname: Janoueix-Lerosey fullname: Janoueix-Lerosey, Isabelle – sequence: 8 givenname: Gordon surname: Langsley fullname: Langsley, Gordon organization: URA CNRS 1960, Département d'Immunologie, Institut Pasteur, 25 Rue du Dr Roux, Cedex 15, 75724, Paris, France – sequence: 9 givenname: Michel surname: Bornens fullname: Bornens, Michel organization: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, Cedex 05, 75248, Paris, France – sequence: 10 givenname: Bruno surname: Goud fullname: Goud, Bruno email: bgoud@curie.fr organization: UMR CNRS 144 et 168, Institut Curie, 26 Rue d'Ulm, Cedex 05, 75248, Paris, France |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10202141$$D View this record in MEDLINE/PubMed |
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| Snippet | The Rab6 GTPase regulates intracellular transport at the level of the Golgi apparatus, probably in a retrograde direction. Here, we report the identification... |
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| SubjectTerms | Amino Acid Sequence Amino acids Animals Base Sequence Carrier Proteins - metabolism centrosome Centrosome - metabolism Cloning, Molecular DNA Primers - genetics Enzyme Activation Fractionation Fungal Proteins - genetics Golgi apparatus GTP Phosphohydrolases - metabolism GTPase activating protein GTPase-Activating Proteins HeLa Cells Humans Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - metabolism mitosis Molecular Sequence Data Proteins - genetics Proteins - metabolism rab GTP-Binding Proteins Rab6 ras GTPase-Activating Proteins ras Proteins - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Schizosaccharomyces - genetics Schizosaccharomyces pombe Sequence Homology, Amino Acid Subcellular Fractions - metabolism Tubulin - metabolism Yeasts |
| Title | Characterization of GAPCenA, a GTPase activating protein for Rab6, part of which associates with the centrosome |
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