Ordered carbohydrate-derived porous carbons immobilized gold nanoparticles as a new electrode material for electrocatalytical oxidation and determination of nicotinamide adenine dinucleotide

• Published in: Biosensors & bioelectronics Vol. 59; pp. 412 - 417
• Main Authors: Hosseini, Hadi, Behbahani, Mohammad, Mahyari, Mojtaba, Kazerooni, Hanif, Bagheri, Akbar, Shaabani, Ahmad
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.09.2014; Elsevier
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensors; Biotechnology; Carbohydrates; Carbon; Carbon - chemistry; Electrochemical biosensor; Electrochemical Techniques - instrumentation; Electrodes; Fundamental and applied biological sciences. Psychology; Gold; Gold - chemistry; Gold nanoparticles; Limit of Detection; Mesoporous carbon materials; Metal Nanoparticles - chemistry; Methods. Procedures. Technologies; NAD - analysis; NADH; Nicotinamide adenine dinucleotide; Ordered carbohydrate-derived porous carbons; Oxidation; Oxidation-Reduction; Porosity; Sensors; Various methods and equipments; Voltammetry; Nicotinamide adenine dinucleotide; Electrochemical biosensor; Ordered carbohydrate-derived porous carbons; Gold nanoparticles; Mesoporous carbon materials; Gold; Nanoparticle; Materials; Carbon; Electrodes; Biosensor; Electrochemistry; Carbohydrate; Nicotinamide; Oxidation
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2014.02.046

The ordered carbohydrate-derived porous carbons (OC-DPCs) were first functionalized with thiol groups (−SH) and then immobilized with gold nanoparticles (AuNPs). The Au-SH-OC-DPCs were characterized by CHN analysis, transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and x-ray diffraction (XRD). The Au-SH-OC-DPCs were applied for the fabrication of a new electrochemical sensor. The electrocatalytic capabilities of the new sensor were tested by the oxidation of nicotinamide adenine dinucleotide (NADH) in a 0.1M Robinson buffer solution (pH 7.0) using cyclic voltammetry (CV), linear sweep voltammetry (LSV), and differential pulse voltammetry (DPV). The Au-SH-OC-DPCs showed a good voltammetric performance in the electrochemical detection of NADH with a low detection limit (1.0nM), high sensitivity (4.934μA/μM), and wide linear concentration range (5.0nM–10µM). •The Ordered carbohydrate-derived porous carbons immobilized gold nanoparticles (Au-SH-OC-DPCs) as a new electrocatalyst.•The Au-SH-OC-DPCs as a new sensor for the electrooxidation of NADH.•The detection limit of 1.0nM and linear concentration range of 5.0nM–10µM for analysis of NADH at Au-SH-OC-DPCs.