Discovery of a highly efficient TylF methyltransferase via random mutagenesis for improving tylosin production

• Published in: Computational and structural biotechnology journal Vol. 21; pp. 2759 - 2766
• Main Authors: Fan, Jingyan, Yao, Zhiming, Yan, Chaoyue, Hao, Meilin, Dai, Jun, Zou, Wenjin, Ni, Minghui, Li, Tingting, Li, Lu, Li, Shuo, Liu, Jie, Huang, Qi, Zhou, Rui
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2023; Research Network of Computational and Structural Biotechnology; Elsevier
• Subjects: biosynthesis; biotechnology; Catalytic activity; class; directed evolution; Directed protein evolution; enzyme activity; fermentation; humans; Macrocin O-methyltransferase; medicine; methyltransferases; mutagenesis; mutants; phenylalanine; protein structure; Rate-limiting enzyme; Streptomyces fradiae; thermal stability; Tylosin; tyrosine; aa; TSB; RU; TSA; Streptomyces fradiae; MTP; Catalytic activity; Rate-limiting enzyme; S. fradiae; SPR; PMSF; LB; Macrocin O-methyltransferase; Directed protein evolution; IPTG; KD; SAH; Tylosin; HPLC; SAM

Macrolides are currently a class of extensively used antibiotics in human and animal medicine. Tylosin is not only one of the most important veterinary macrolides but also an indispensable material for the bio- and chemo-synthesis of new generations of macrolide antibiotics. Thus, improving its prod...