Discovery of a highly efficient TylF methyltransferase via random mutagenesis for improving tylosin production - Fan, Jingyan; Yao, Zhiming; Yan, Chaoyue; Hao, Meilin; Dai, Jun; Zou, Wenjin; Ni, Minghui; Li, Tingting; Li, Lu; Li, Shuo; Liu, Jie; Huang, Qi; et al. (Computational and structural biotechnology journal, 2023)
Keywords: biosynthesis; biotechnology; Catalytic activity; class; directed evolution; Directed protein evolution; enzyme activity; fermentation; humans; Macrocin O-methyltransferase; medicine; methyltransferases; mutagenesis; mutants; phenylalanine; protein structure; Rate-limiting enzyme; Streptomyces fradiae; thermal stability; Tylosin; tyrosine; aa; TSB; RU; TSA; Streptomyces fradiae; MTP; Catalytic activity; Rate-limiting enzyme; S. fradiae; SPR; PMSF; LB; Macrocin O-methyltransferase; Directed protein evolution; IPTG; KD; SAH; Tylosin; HPLC; SAM