Effects of cilostamide and forskolin on the meiotic resumption and embryonic development of immature human oocytes

• Published in: Human reproduction (Oxford) Vol. 23; no. 3; pp. 504 - 513
• Main Authors: Shu, Yi-min, Zeng, Hai-tao, Ren, Zi, Zhuang, Guang-lun, Liang, Xiao-yan, Shen, Hong-wei, Yao, Shu-zhong, Ke, Pei-qi, Wang, Ning-ning
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.03.2008; Oxford Publishing Limited (England)
• Subjects: Adenylyl Cyclases - metabolism; Adult; Biological and medical sciences; Cells, Cultured; cilostamide; Colforsin - pharmacology; cytoplasmic maturation; Drug Synergism; Embryonic Development - drug effects; Female; forskolin; Gap Junctions - drug effects; Gap Junctions - physiology; Gynecology. Andrology. Obstetrics; human oocyte; Humans; Medical sciences; Meiosis - drug effects; meiotic resumption; Oocytes - cytology; Oocytes - drug effects; Phosphodiesterase Inhibitors - pharmacology; Quinolones - pharmacology; meiotic resumption; forskolin; human oocyte; cytoplasmic maturation; cilostamide; Human; Embryonic development; 3',5'-Cyclic-nucleotide phosphodiesterase; Enzyme; Enzyme inhibitor; Esterases; Phosphoric diester hydrolases; Germinal cell; Phosphodiesterase inhibitor; Cilostamide; Ripening; Vertebrata; Mammalia; Hydrolases; Oocyte

BACKGROUND In an attempt to allow for acquisition of oocyte cytoplasmic maturation, PDE3 specific inhibitor, cilostamide and adenylate cyclase activator, forskolin were used to extend pre-maturation culture of immature human oocytes. METHODS Cumulus–oocyte complexes retrieved from unstimulated ovari...