Stromal fibroblasts shape the myeloid phenotype in normal colon and colorectal cancer and induce CD163 and CCL2 expression in macrophages

• Published in: Cancer letters Vol. 520; pp. 184 - 200
• Main Authors: Stadler, Mira, Pudelko, Karoline, Biermeier, Alexander, Walterskirchen, Natalie, Gaigneaux, Anthoula, Weindorfer, Claudia, Harrer, Nathalie, Klett, Hagen, Hengstschläger, Markus, Schüler, Julia, Sommergruber, Wolfgang, Oehler, Rudolf, Bergmann, Michael, Letellier, Elisabeth, Dolznig, Helmut
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.11.2021; Elsevier Limited
• Subjects: Angiogenesis; Antigens; Antigens, CD - genetics; Antigens, Differentiation, Myelomonocytic - genetics; Cancer associated fibroblasts; Cancer-Associated Fibroblasts - metabolism; Cancer-Associated Fibroblasts - pathology; Carcinogenesis; CCL2; CCR2 protein; CD163 antigen; Cell adhesion & migration; Cell culture; Cell Differentiation - genetics; Cell Movement - genetics; Cell Proliferation - genetics; Chemokine CCL2 - genetics; Collagen; Colon - metabolism; Colon - pathology; Colon cancer; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - blood; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Female; Fibroblasts; Flow cytometry; Gene Expression Regulation, Neoplastic - genetics; HCT116 Cells; Homeostasis; Humans; Immune cell recruitment and polarization; Interleukin 6; Interleukin 8; Macrophage colony-stimulating factor; Macrophage Colony-Stimulating Factor - genetics; Macrophages; Male; Medical prognosis; Monocyte chemoattractant protein 1; Monocytes; Myeloid Cells - metabolism; Myeloid Cells - pathology; Phenotypes; Pore size; Receptors, Cell Surface - genetics; Signal Transduction - genetics; Spheroids; Tumor cells; Tumor microenvironment; Tumor Microenvironment - genetics; United States > US; Germany; CCL2; Colon cancer; Cancer associated fibroblasts; Tumor microenvironment; Immune cell recruitment and polarization

Colorectal cancer (CRC) accounts for about 10% of cancer deaths worldwide. Colon carcinogenesis is critically influenced by the tumor microenvironment. Cancer associated fibroblasts (CAFs) and tumor associated macrophages (TAMs) represent the major components of the tumor microenvironment. TAMs prom...