Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients
Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethylligand extracts in allergic patients and healthy...
Saved in:
| Published in | Brazilian journal of medical and biological research Vol. 56; no. 1; p. e12957 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English Portuguese |
| Published |
Associacao Brasileira de Divulgacao Cientifica (ABDC)
01.01.2023
Associação Brasileira de Divulgação Científica |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethylligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non- atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed thatcarboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy. Key words: Allergen; Ion-exchange chromatography; Beta-expansin; Lolium multiflorum; IgE; IgG4 |
|---|---|
| AbstractList | Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethyl-ligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2-specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non-atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed that carboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy. Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethyl-ligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2-specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non-atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed that carboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy. Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethylligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non- atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed thatcarboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy. Key words: Allergen; Ion-exchange chromatography; Beta-expansin; Lolium multiflorum; IgE; IgG4 Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim of this study was to investigate antibody reactivity to L. multiflorum crude and carboxymethylligand extracts in allergic patients and healthy individuals. Ion exchange carboxymethyl (CM) chromatography (CM-Sepharose) was used to isolate proteins (S2) from L. multiflorum crude extract (S1), which were assessed by SDS-PAGE. S1- and S2specific IgE and IgG4 levels were measured by ELISA using sera from 55 atopic and 16 non- atopic subjects. Reactive polypeptide bands in S1 and S2 were detected by immunoblotting, and the most prominent bands in S2 were analyzed by mass spectrometry (MS-MS). Similar IgE and IgG4 levels were observed to both S1 (IgE median absorbance: 1.22; IgG4 median absorbance: 0.68) and S2 (IgE median absorbance: 1.26; IgG4 median absorbance: 0.85) in atopic subjects. S1 and S2 had positive correlations for IgE and IgG4 (IgE: r=0.9567; IgG4: r=0.9229; P<0.0001) levels. Homology between S1 and S2 was confirmed by IgE (84%) and IgG4 (83%) inhibition. Immunoblotting revealed that the 29-32 kDa band was recognized by 100% of atopic subjects in both S1 and S2. MS-MS analysis identified similarity profile to groups 1 and 5 grass allergens. This study revealed thatcarboxymethyl-ligand fraction played an important role for pollen allergy diagnosis by containing clinically relevant allergens and constituted a promising candidate for allergen-specific immunotherapy. |
| Audience | Academic |
| Author | Resende, R.O Correa, A.S Taketomi, E.A Moreira, P.F.S Cunha, J.P., Jr Miranda, J.S Oliveira, L.A.R Vieira, F.A.M |
| AuthorAffiliation | Universidade de Caxias do Sul Instituto Oswaldo Cruz, Fiocruz Universidade Federal de Uberlândia |
| AuthorAffiliation_xml | – name: Universidade de Caxias do Sul – name: Instituto Oswaldo Cruz, Fiocruz – name: Universidade Federal de Uberlândia |
| Author_xml | – sequence: 1 fullname: Correa, A.S – sequence: 2 fullname: Miranda, J.S – sequence: 3 fullname: Oliveira, L.A.R – sequence: 4 fullname: Moreira, P.F.S – sequence: 5 fullname: Vieira, F.A.M – sequence: 6 fullname: Cunha, J.P., Jr – sequence: 7 fullname: Resende, R.O – sequence: 8 fullname: Taketomi, E.A |
| BookMark | eNpVUk1v1DAQjVCR2BZ-ARdLXMohxR-Jk5xQWRVYaRGHcujNcuzJdlaOvTjJape_wp_FYatWK8uyPZ73_Gb8LrMLHzxk2XtGb1jZ0E-sYEVeCPbAKRfAeFNWr7LFc_QiW1BGaV5XzcOb7HIYtpTykhZskf1dWfAjdmj0iMGT0BGjYxsOxx7Gx6Mj18sfH_MWvUW_IbsYRkA_EAsR92BJF0NP1sHh1JN-conIhZj2u-AceAKHMWozEu1tmiO2wR5JhBTCPY5Hgp58ifoPOtSe6ASJGzRkl6QkUcPb7HWn3QDvntar7P7r3a_l93z989tqebvOTVmWY65bS6m1WkrR6JJDJTQUBmQjjDG8YVq0LU2NkVUnuGCy1rK2QvJGC-hqcZWtTqw26K3aRex1PKqgUf0PhLhROo5oHKim7aSU0HArikI2bV3rojLQlLxmZS114ro5cQ0GwQW1DVP0Sbq6n_uv5v7PP0TpfJQVTYDPJ8BuanuwJpUdtTtTcX7j8VFtwl4xWlY14zPD9RNDDL8nGEbV42DAOe0hTIPi6dGCJn1VSv1wSt3oVAz6Lsy_M6er26oRrOaiZi81nGWlYaFHk3zXYYqfAcQJYGIYhgjds3xG1WxPNRtRJSMeXuwp_gFWZdrP |
| CitedBy_id | crossref_primary_10_3390_vaccines12040408 |
| Cites_doi | 10.1016/0003-2697(76)90527-3 10.1111/j.1467-7652.2011.00654.x 10.1016/j.jaci.2018.09.039 10.1016/S1808-8694(15)31005-3 10.1016/j.ebiom.2019.11.006 10.1111/cea.12317 10.1007/s12016-018-8712-1 10.1172/JCI119330 10.1016/j.aller.2015.03.001 10.1590/S0100-879X2010005000004 10.1007/s00709-006-0176-0 10.1159/000464104 10.1016/S0031-3955(16)36544-0 10.1016/S1808-8694(15)31262-3 10.1371/journal.pone.0128402 10.1016/0021-8707(62)90067-9 10.7748/ns.2016.e9220 10.1073/pnas.76.9.4350 10.1002/pmic.200401038 10.1038/227680a0 10.1111/all.13210 10.1046/j.1432-1327.1999.00462.x 10.1104/pp.103.020024 10.1155/2019/9840890 10.1159/000092531 10.1016/j.imlet.2017.04.015 10.1016/j.pop.2016.04.009 10.1111/all.12696 10.1016/j.arbr.2018.12.020 10.1186/s12948-019-0110-6 10.1016/j.homp.2015.12.063 10.29328/journal.aaai.1001024 10.1159/000321821 10.1016/j.molimm.2018.03.012 10.1016/j.molimm.2008.08.267 10.1001/archopht.1990.01070030090035 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2023 Associacao Brasileira de Divulgacao Cientifica (ABDC) This work is licensed under a Creative Commons Attribution 4.0 International License. |
| Copyright_xml | – notice: COPYRIGHT 2023 Associacao Brasileira de Divulgacao Cientifica (ABDC) – notice: This work is licensed under a Creative Commons Attribution 4.0 International License. |
| DBID | AAYXX CITATION INF 7X8 5PM GPN DOA |
| DOI | 10.1590/1414-431X2023e12957 |
| DatabaseName | CrossRef Gale OneFile: Informe Academico MEDLINE - Academic PubMed Central (Full Participant titles) SciELO DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE - Academic |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Biology |
| EISSN | 1414-431X |
| EndPage | e12957 |
| ExternalDocumentID | oai_doaj_org_article_9bf666e92d34469b88a47ce95281586a S0100_879X2023000100670 A793182381 10_1590_1414_431x2023e12957 |
| GeographicLocations | Brazil |
| GeographicLocations_xml | – name: Brazil |
| GroupedDBID | --- 23N 2WC 53G 5GY 5VS 6J9 AAYXX ABIVO ABXHO ACGFO ADBBV ADRAZ AENEX AFPKN AIAGR ALMA_UNASSIGNED_HOLDINGS AOIJS APOWU AZFZN BAWUL BCNDV C1A CITATION CS3 DIK E3Z EBD EBS EJD EMOBN FRP GROUPED_DOAJ GX1 HYE IAO IEA IHR IHW INF INH INR IPNFZ ITC KQ8 M48 M~E OK1 P2P P6G PGMZT PV9 RIG RNS RPM RSC RZL SCD SV3 TR2 TUS W2D XSB ZXP 7X8 5PM GPN |
| ID | FETCH-LOGICAL-c555t-abd00dda6639a52e73ae4ce693ccc291a3bb020267f323168a68d3629a3ef83 |
| IEDL.DBID | RPM |
| ISSN | 0100-879X 1414-431X |
| IngestDate | Fri Oct 04 13:07:29 EDT 2024 Fri Oct 18 20:39:28 EDT 2024 Tue Sep 17 21:29:21 EDT 2024 Fri Aug 16 01:01:28 EDT 2024 Wed May 29 17:41:19 EDT 2024 Tue Jun 04 11:53:08 EDT 2024 Fri Aug 23 02:09:46 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | IgG4 Beta-expansin Ion-exchange chromatography Lolium multiflorum IgE Allergen |
| Language | English Portuguese |
| License | This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This work is licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0 |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c555t-abd00dda6639a52e73ae4ce693ccc291a3bb020267f323168a68d3629a3ef83 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0003-4854-6822 0009-0008-5749-5629 0000-0003-1446-5063 0000-0002-1370-0714 0000-0001-8509-8964 0000-0002-6711-6353 0000-0002-2182-0863 0000-0001-6182-4298 |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578120/ |
| PQID | 2879408157 |
| PQPubID | 23479 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_9bf666e92d34469b88a47ce95281586a scielo_journals_S0100_879X2023000100670 pubmedcentral_primary_oai_pubmedcentral_nih_gov_10578120 proquest_miscellaneous_2879408157 gale_infotracmisc_A793182381 gale_infotracacademiconefile_A793182381 crossref_primary_10_1590_1414_431x2023e12957 |
| PublicationCentury | 2000 |
| PublicationDate | 2023-01-01 |
| PublicationDateYYYYMMDD | 2023-01-01 |
| PublicationDate_xml | – month: 01 year: 2023 text: 2023-01-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationTitle | Brazilian journal of medical and biological research |
| PublicationTitleAlternate | Braz J Med Biol Res |
| PublicationYear | 2023 |
| Publisher | Associacao Brasileira de Divulgacao Cientifica (ABDC) Associação Brasileira de Divulgação Científica |
| Publisher_xml | – name: Associacao Brasileira de Divulgacao Cientifica (ABDC) – name: Associação Brasileira de Divulgação Científica |
| References | Ownby DR (ref21) 1988; 35 Abelson MB (ref29) 1990; 108 Bernardes CT (ref10) 2010; 43 Kuruvilla ME (ref26) 2019; 56 García-Mozo H (ref17) 2017; 72 Laemmli UK (ref24) 1970; 227 Taketomi EA (ref39) 2017 Kailaivasan T (ref18) 2018; 100 de Souza CCT (ref19) 2021; 5 Asam C (ref6) 2015; 70 Shamji MH (ref38) 2019; 143 Kakli HA (ref1) 2016; 43 Silva LVER (ref8) 2005; 71 Vrtala S (ref35) 1997; 99 Sterner T (ref33) 2019; 17 Malley A (ref14) 1962; 33 Jin Y (ref31) 2006; 228 Bartle J (ref2) 2016; 30 Vizuete JAC (ref4) 2019; 55 Valenta R (ref37) 2017; 189 Towbin H (ref25) 1979; 76 Davies JM (ref16) 2008; 46 Davies JM (ref15) 2014; 44 Resende RO (ref23) 2019; 2019 Grobe K (ref30) 1999; 263 Taketomi EA (ref28) 2006; 72 Tscheppe A (ref34) 2017; 172 Drew AC (ref12) 2011; 154 Manduzio H (ref32) 2012; 10 Moreira PFS (ref11) 2015; 10 Bradford MM (ref22) 1976; 72 Corti V (ref7) 2005; 5 Sopelete MC (ref27) 2006; 140 Pandey V (ref5) 2016; 105 Bartra J (ref3) 2009; 19 Araujo LML (ref9) 2016; 44 Eckl-Dorna J (ref36) 2019; 50 Li LC (ref13) 2003; 132 Pacharn P (ref20) 2019; 37 Asam, C; Hofer, H; Wolf, M; Aglas, L; Wallner, M 2015; 70 Valenta, R; Campana, R; Niederberger, V 2017; 189 Sterner, T; Uldahl, A; Svensson, A; Borres, MP; Sjölander, S; Tunsäter, A 2019; 17 Pandey, V 2016; 105 Corti, V; Cattaneo, A; Bachi, A; Rossi, RE; Monasterolo, G; Paolucci, C 2005; 5 Kuruvilla, ME; Lee, FEH; Lee, GB 2019; 56 Davies, JM; Mittag, D; Dang, TD; Symons, K; Voskamp, A; Rolland, JM 2008; 46 Grobe, K; Becker, WM; Schlaak, M; Petersen, A 1999; 263 Bartra, J; Sastre, J; del Cuvillo, A; Montoro, J; Jáuregui, I; Dávila, I 2009; 19 Silva, LVER; de Mello Jr, JF; Mion, O 2005; 71 Drew, AC; Davies, JM; Dang, TD; Rolland, JM; O'Hehir, RE 2011; 154 Resende, RO; Ynoue, LH; Miranda, JS; de Almeida, KC; Silva, DAO; Sopelete, MC 2019; 2019 Malley, A; Reed, CE; Lietze, A 1962; 33 Manduzio, H; Fitchette, AC; Hrabina, M; Chabre, H; Batard, T; Nony, E 2012; 10 Davies, JM 2014; 44 Bradford, MM 1976; 72 Kailaivasan, T; Davies, JM 2018; 100 Bernardes, CT; Moreira, PF; Sopelete, MC; Vieira, FA; Sung, SS; Silva, DA 2010; 43 Laemmli, UK 1970; 227 Vizuete, JAC; Sastre, J; Bernal, ADC; Picado, C; Moragón, EM; García, JMI 2019; 55 García-Mozo, H 2017; 72 Sopelete, MC; Moreira, PFS; Silva, DAO; Cunha-Júnior, JP; Vieira, FAM; Sung, SSJ 2006; 140 Taketomi, EA; Sopelete, MC; Moreira, PFS; Vieira, FAM 2006; 72 Bartle, J 2016; 30 Li, LC; Bedinger, PA; Volk, C; Jones, AD; Cosgrove, DJ 2003; 132 Pacharn, P; Songnual, W; Siriwatanakul, U; Thongngarm, T; Reamtong, O; Jirapongsananuruk, O 2019; 37 Araujo, LML; Rosario, NA; Mari, A 2016; 44 Ownby, DR 1988; 35 Shamji, MH; Kappen, J; Abubakar-Waziri, H; Zhang, J; Steveling, E; Watchman, S 2019; 143 Towbin, H; Staehelin, T; Gordon, J 1979; 76 Moreira, PFS; Gangl, K; Vieira, FAM; Ynoue, LH; Linhart, B; Flicker, S 2015; 10 de Souza, CCT; Rosario Filho, NA; Taketomi, EA; Miranda, JS; Godoi, RHM 2021; 5 Abelson, MB; Chambers, WA; Smith, LM 1990; 108 Jin, Y; Tashpulatov, AS; Katholnigg, H; Heberle-Bors, E; Touraev, A 2006; 228 Tscheppe, A; Breiteneder, H 2017; 172 Vrtala, S; Hirtenlehner, K; Vangelista, L; Pastore, A; Eichler, HG; Sperr, WR 1997; 99 Eckl-Dorna, J; Weber, M; Stanek, V; Linhart, B; Ristl, R; Waltl, EE 2019; 50 Kakli, HA; Riley, TD 2016; 43 Taketomi, EA; Miranda, JS; Júnior, JPC; Silva, DAO; Metodiev, K 2017 |
| References_xml | – volume: 72 start-page: 248 year: 1976 ident: ref22 article-title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding publication-title: Anal Biochem doi: 10.1016/0003-2697(76)90527-3 contributor: fullname: Bradford MM – volume: 10 start-page: 184 year: 2012 ident: ref32 article-title: Glycoproteins are species-specific markers and major IgE reactants in grass pollens publication-title: Plant Biotechnol J doi: 10.1111/j.1467-7652.2011.00654.x contributor: fullname: Manduzio H – start-page: 381 volume-title: Allergen-specific immunotherapy follow-up by measuring allergen-specific IgG as an objective parameter year: 2017 ident: ref39 contributor: fullname: Taketomi EA – volume: 143 start-page: 1067 year: 2019 ident: ref38 article-title: Nasal allergen-neutralizing IgG(4) antibodies block IgE-mediated responses: novel biomarker of subcutaneous grass pollen immunotherapy publication-title: J Allergy Clin Immunol doi: 10.1016/j.jaci.2018.09.039 contributor: fullname: Shamji MH – volume: 72 start-page: 4 year: 2006 ident: ref28 article-title: Pollen allergic disease: pollens and its major allergens publication-title: Braz J Otorhinolaryngol doi: 10.1016/S1808-8694(15)31005-3 contributor: fullname: Taketomi EA – volume: 50 year: 2019 ident: ref36 article-title: Two years of treatment with the recombinant grass pollen allergy vaccine BM32 induces a continuously increasing allergen-specific IgG4 response publication-title: EBioMedicine doi: 10.1016/j.ebiom.2019.11.006 contributor: fullname: Eckl-Dorna J – volume: 44 start-page: 790 year: 2014 ident: ref15 article-title: Grass pollen allergens globally: the contribution of subtropical grasses to burden of allergic respiratory diseases publication-title: Clin Exp Allergy doi: 10.1111/cea.12317 contributor: fullname: Davies JM – volume: 56 start-page: 219 year: 2019 ident: ref26 article-title: Understanding asthma phenotypes, endotypes, and mechanisms of disease publication-title: Clinl Rev Allergy Immunol doi: 10.1007/s12016-018-8712-1 contributor: fullname: Kuruvilla ME – volume: 99 start-page: 1673 year: 1997 ident: ref35 article-title: Conversion of the major birch pollen allergen, Bet v 1, into two nonanaphylactic T cell epitope-containing fragments: candidates for a novel form of specific immunotherapy publication-title: J Clin Invest doi: 10.1172/JCI119330 contributor: fullname: Vrtala S – volume: 44 start-page: 18 year: 2016 ident: ref9 article-title: Molecular-based diagnosis of respiratory allergic diseases in children from Curitiba, a city in Southern Brazil publication-title: Allergol Immunopathol (Madr) doi: 10.1016/j.aller.2015.03.001 contributor: fullname: Araujo LML – volume: 37 start-page: 30 year: 2019 ident: ref20 article-title: Beta-expansin of Bermuda, Johnson, and Para grass pollens, is a major cross-reactive allergen for Allergic Rhinitis patients in subtropical climate publication-title: Asian Pac J Allergy Immunol contributor: fullname: Pacharn P – volume: 43 start-page: 166 year: 2010 ident: ref10 article-title: IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis publication-title: Braz J Med Biol Res doi: 10.1590/S0100-879X2010005000004 contributor: fullname: Bernardes CT – volume: 228 start-page: 13 year: 2006 ident: ref31 article-title: Isolation and characterisation of two wheat beta-expansin genes expressed during male gametophyte development publication-title: Protoplasma doi: 10.1007/s00709-006-0176-0 contributor: fullname: Jin Y – volume: 172 start-page: 187 year: 2017 ident: ref34 article-title: Recombinant allergens in structural biology, diagnosis, and immunotherapy publication-title: Int Arch Allergy Immunol doi: 10.1159/000464104 contributor: fullname: Tscheppe A – volume: 35 start-page: 995 year: 1988 ident: ref21 article-title: Allergy testing: in vivo versus in vitro publication-title: Pediatr Clin North Am doi: 10.1016/S0031-3955(16)36544-0 contributor: fullname: Ownby DR – volume: 71 start-page: 590 year: 2005 ident: ref8 article-title: Evaluation of Brazilian web site information on allergic rhinitis publication-title: Braz J Otorhinolaryngol doi: 10.1016/S1808-8694(15)31262-3 contributor: fullname: Silva LVER – volume: 10 start-page: e0128402 year: 2015 ident: ref11 article-title: Allergen microarray indicates pooideae sensitization in Brazilian publication-title: PLoS One doi: 10.1371/journal.pone.0128402 contributor: fullname: Moreira PFS – volume: 33 start-page: 84 year: 1962 ident: ref14 article-title: Isolation of allergens from timothy pollen publication-title: J Allergy doi: 10.1016/0021-8707(62)90067-9 contributor: fullname: Malley A – volume: 30 start-page: 48 year: 2016 ident: ref2 article-title: Patient education in the effective management of hay fever publication-title: Nurs Stand doi: 10.7748/ns.2016.e9220 contributor: fullname: Bartle J – volume: 76 start-page: 4350 year: 1979 ident: ref25 article-title: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.76.9.4350 contributor: fullname: Towbin H – volume: 5 start-page: 729 year: 2005 ident: ref7 article-title: Identification of grass pollen allergens by two-dimensional gel electrophoresis and serological screening publication-title: Proteomics doi: 10.1002/pmic.200401038 contributor: fullname: Corti V – volume: 227 start-page: 680 year: 1970 ident: ref24 article-title: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 publication-title: Nature doi: 10.1038/227680a0 contributor: fullname: Laemmli UK – volume: 72 start-page: 1849 year: 2017 ident: ref17 article-title: Poaceae pollen as the leading aeroallergen worldwide: a review publication-title: Allergy doi: 10.1111/all.13210 contributor: fullname: García-Mozo H – volume: 263 start-page: 33 year: 1999 ident: ref30 article-title: Grass group I allergens (beta-expansins) are novel, papain-related proteinases publication-title: Eur J Biochem doi: 10.1046/j.1432-1327.1999.00462.x contributor: fullname: Grobe K – volume: 132 start-page: 2073 year: 2003 ident: ref13 article-title: Purification and characterization of four beta-expansins (Zea m 1 isoforms) from maize pollen publication-title: Plant Physiol doi: 10.1104/pp.103.020024 contributor: fullname: Li LC – volume: 2019 start-page: 9840890 year: 2019 ident: ref23 article-title: IgE, IgG1, and IgG4 reactivity to Dermatophagoides pteronyssinus glycosylated extract in allergic patients publication-title: Biomed Res Int doi: 10.1155/2019/9840890 contributor: fullname: Resende RO – volume: 140 start-page: 121 year: 2006 ident: ref27 article-title: Sensitization to Lolium multiflorum grass pollen in pollinosis patients: evaluation of allergenic fractions recognized by specific IgE antibodies publication-title: Int Arch Allergy Immunol doi: 10.1159/000092531 contributor: fullname: Sopelete MC – volume: 189 start-page: 19 year: 2017 ident: ref37 article-title: Recombinant allergy vaccines based on allergen-derived B cell epitopes publication-title: Immunol Lett doi: 10.1016/j.imlet.2017.04.015 contributor: fullname: Valenta R – volume: 19 start-page: 3 year: 2009 ident: ref3 article-title: From pollinosis to digestive allergy publication-title: J Investig Allergol Clin Immunol contributor: fullname: Bartra J – volume: 43 start-page: 465 year: 2016 ident: ref1 article-title: Allergic rhinitis publication-title: Prim Care doi: 10.1016/j.pop.2016.04.009 contributor: fullname: Kakli HA – volume: 70 start-page: 1201 year: 2015 ident: ref6 article-title: Tree pollen allergens-an update from a molecular perspective publication-title: Allergy doi: 10.1111/all.12696 contributor: fullname: Asam C – volume: 55 start-page: 146 year: 2019 ident: ref4 article-title: Asthma, rhinitis, and nasal polyp multimorbidities publication-title: Arch Bronconeumol (Engl Ed) doi: 10.1016/j.arbr.2018.12.020 contributor: fullname: Vizuete JAC – volume: 17 start-page: 6 year: 2019 ident: ref33 article-title: IgE sensitization in a cohort of adolescents in southern Sweden and its relation to allergic symptoms publication-title: Clin Mol Allergy doi: 10.1186/s12948-019-0110-6 contributor: fullname: Sterner T – volume: 105 start-page: 202 year: 2016 ident: ref5 article-title: Hay fever & homeopathy: a case series evaluation publication-title: Homeopathy doi: 10.1016/j.homp.2015.12.063 contributor: fullname: Pandey V – volume: 5 start-page: 14 year: 2021 ident: ref19 article-title: Cyn d 1 airborne allergen in a Southern Brazilian city publication-title: Arch Asthma Allergy Immunol doi: 10.29328/journal.aaai.1001024 contributor: fullname: de Souza CCT – volume: 154 start-page: 295 year: 2011 ident: ref12 article-title: Purification of the major group 1 allergen from Bahia grass pollen, Pas n 1 publication-title: Int Arch Allergy Immunol doi: 10.1159/000321821 contributor: fullname: Drew AC – volume: 100 start-page: 126 year: 2018 ident: ref18 article-title: The molecular allergology of subtropical grass pollen publication-title: Mol Immunol doi: 10.1016/j.molimm.2018.03.012 contributor: fullname: Kailaivasan T – volume: 46 start-page: 286 year: 2008 ident: ref16 article-title: Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahia grass Paspalum notatum pollen publication-title: Mol Immunol doi: 10.1016/j.molimm.2008.08.267 contributor: fullname: Davies JM – volume: 108 start-page: 84 year: 1990 ident: ref29 article-title: Conjunctival allergen challenge: a clinical approach to studying allergic conjunctivitis publication-title: Arch Ophthalmol doi: 10.1001/archopht.1990.01070030090035 contributor: fullname: Abelson MB – volume: 46 start-page: 286 year: 2008 end-page: 293 article-title: Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahia grass Paspalum notatum pollen publication-title: Mol Immunol contributor: fullname: Davies, JM; Mittag, D; Dang, TD; Symons, K; Voskamp, A; Rolland, JM – volume: 227 start-page: 680 year: 1970 end-page: 685 article-title: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 publication-title: Nature contributor: fullname: Laemmli, UK – volume: 76 start-page: 4350 year: 1979 end-page: 4354 article-title: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications publication-title: Proc Natl Acad Sci USA contributor: fullname: Towbin, H; Staehelin, T; Gordon, J – start-page: 381 year: 2017 end-page: 394 publication-title: Immunotherapy - Myths, Reality, Ideas, Future contributor: fullname: Taketomi, EA; Miranda, JS; Júnior, JPC; Silva, DAO; Metodiev, K – volume: 228 start-page: 13 year: 2006 end-page: 19 article-title: Isolation and characterisation of two wheat beta-expansin genes expressed during male gametophyte development publication-title: Protoplasma contributor: fullname: Jin, Y; Tashpulatov, AS; Katholnigg, H; Heberle-Bors, E; Touraev, A – volume: 43 start-page: 166 year: 2010 end-page: 175 article-title: IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis publication-title: Braz J Med Biol Res contributor: fullname: Bernardes, CT; Moreira, PF; Sopelete, MC; Vieira, FA; Sung, SS; Silva, DA – volume: 35 start-page: 995 year: 1988 end-page: 1009 article-title: Allergy testing: in vivo versus in vitro publication-title: Pediatr Clin North Am contributor: fullname: Ownby, DR – volume: 10 start-page: 184 year: 2012 end-page: 194 article-title: Glycoproteins are species-specific markers and major IgE reactants in grass pollens publication-title: Plant Biotechnol J contributor: fullname: Manduzio, H; Fitchette, AC; Hrabina, M; Chabre, H; Batard, T; Nony, E – volume: 70 start-page: 1201 year: 2015 end-page: 1211 article-title: Tree pollen allergens-an update from a molecular perspective publication-title: Allergy contributor: fullname: Asam, C; Hofer, H; Wolf, M; Aglas, L; Wallner, M – volume: 44 start-page: 790 year: 2014 end-page: 801 article-title: Grass pollen allergens globally: the contribution of subtropical grasses to burden of allergic respiratory diseases publication-title: Clin Exp Allergy contributor: fullname: Davies, JM – volume: 154 start-page: 295 year: 2011 end-page: 298 article-title: Purification of the major group 1 allergen from Bahia grass pollen, Pas n 1 publication-title: Int Arch Allergy Immunol contributor: fullname: Drew, AC; Davies, JM; Dang, TD; Rolland, JM; O'Hehir, RE – volume: 5 start-page: 14 year: 2021 end-page: 16 article-title: Cyn d 1 airborne allergen in a Southern Brazilian city publication-title: Arch Asthma Allergy Immunol contributor: fullname: de Souza, CCT; Rosario Filho, NA; Taketomi, EA; Miranda, JS; Godoi, RHM – volume: 2019 start-page: 9840890 year: 2019 article-title: IgE, IgG1, and IgG4 reactivity to Dermatophagoides pteronyssinus glycosylated extract in allergic patients publication-title: Biomed Res Int contributor: fullname: Resende, RO; Ynoue, LH; Miranda, JS; de Almeida, KC; Silva, DAO; Sopelete, MC – volume: 43 start-page: 465 year: 2016 end-page: 475 article-title: Allergic rhinitis publication-title: Prim Care contributor: fullname: Kakli, HA; Riley, TD – volume: 55 start-page: 146 year: 2019 end-page: 155 article-title: Asthma, rhinitis, and nasal polyp multimorbidities publication-title: Arch Bronconeumol (Engl Ed) contributor: fullname: Vizuete, JAC; Sastre, J; Bernal, ADC; Picado, C; Moragón, EM; García, JMI – volume: 56 start-page: 219 year: 2019 end-page: 233 article-title: Understanding asthma phenotypes, endotypes, and mechanisms of disease publication-title: Clinl Rev Allergy Immunol contributor: fullname: Kuruvilla, ME; Lee, FEH; Lee, GB – volume: 71 start-page: 590 year: 2005 end-page: 597 article-title: Evaluation of Brazilian web site information on allergic rhinitis publication-title: Braz J Otorhinolaryngol contributor: fullname: Silva, LVER; de Mello Jr, JF; Mion, O – volume: 72 start-page: 1849 year: 2017 end-page: 1858 article-title: Poaceae pollen as the leading aeroallergen worldwide: a review publication-title: Allergy contributor: fullname: García-Mozo, H – volume: 140 start-page: 121 year: 2006 end-page: 130 article-title: Sensitization to Lolium multiflorum grass pollen in pollinosis patients: evaluation of allergenic fractions recognized by specific IgE antibodies publication-title: Int Arch Allergy Immunol contributor: fullname: Sopelete, MC; Moreira, PFS; Silva, DAO; Cunha-Júnior, JP; Vieira, FAM; Sung, SSJ – volume: 263 start-page: 33 year: 1999 end-page: 40 article-title: Grass group I allergens (beta-expansins) are novel, papain-related proteinases publication-title: Eur J Biochem contributor: fullname: Grobe, K; Becker, WM; Schlaak, M; Petersen, A – volume: 105 start-page: 202 year: 2016 end-page: 208 article-title: Hay fever & homeopathy: a case series evaluation publication-title: Homeopathy contributor: fullname: Pandey, V – volume: 44 start-page: 18 year: 2016 end-page: 22 article-title: Molecular-based diagnosis of respiratory allergic diseases in children from Curitiba, a city in Southern Brazil publication-title: Allergol Immunopathol (Madr) contributor: fullname: Araujo, LML; Rosario, NA; Mari, A – volume: 143 start-page: 1067 year: 2019 end-page: 1076 article-title: Nasal allergen-neutralizing IgG(4) antibodies block IgE-mediated responses: novel biomarker of subcutaneous grass pollen immunotherapy publication-title: J Allergy Clin Immunol contributor: fullname: Shamji, MH; Kappen, J; Abubakar-Waziri, H; Zhang, J; Steveling, E; Watchman, S – volume: 19 start-page: 3 year: 2009 end-page: 10 article-title: From pollinosis to digestive allergy publication-title: J Investig Allergol Clin Immunol contributor: fullname: Bartra, J; Sastre, J; del Cuvillo, A; Montoro, J; Jáuregui, I; Dávila, I – volume: 132 start-page: 2073 year: 2003 end-page: 2085 article-title: Purification and characterization of four beta-expansins (Zea m 1 isoforms) from maize pollen publication-title: Plant Physiol contributor: fullname: Li, LC; Bedinger, PA; Volk, C; Jones, AD; Cosgrove, DJ – volume: 30 start-page: 48 year: 2016 end-page: 53 article-title: Patient education in the effective management of hay fever publication-title: Nurs Stand contributor: fullname: Bartle, J – volume: 100 start-page: 126 year: 2018 end-page: 135 article-title: The molecular allergology of subtropical grass pollen publication-title: Mol Immunol contributor: fullname: Kailaivasan, T; Davies, JM – volume: 72 start-page: 248 year: 1976 end-page: 254 article-title: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding publication-title: Anal Biochem contributor: fullname: Bradford, MM – volume: 99 start-page: 1673 year: 1997 end-page: 1681 article-title: Conversion of the major birch pollen allergen, Bet v 1, into two nonanaphylactic T cell epitope-containing fragments: candidates for a novel form of specific immunotherapy publication-title: J Clin Invest contributor: fullname: Vrtala, S; Hirtenlehner, K; Vangelista, L; Pastore, A; Eichler, HG; Sperr, WR – volume: 108 start-page: 84 year: 1990 end-page: 88 article-title: Conjunctival allergen challenge: a clinical approach to studying allergic conjunctivitis publication-title: Arch Ophthalmol contributor: fullname: Abelson, MB; Chambers, WA; Smith, LM – volume: 33 start-page: 84 year: 1962 end-page: 93 article-title: Isolation of allergens from timothy pollen publication-title: J Allergy contributor: fullname: Malley, A; Reed, CE; Lietze, A – volume: 172 start-page: 187 year: 2017 end-page: 202 article-title: Recombinant allergens in structural biology, diagnosis, and immunotherapy publication-title: Int Arch Allergy Immunol contributor: fullname: Tscheppe, A; Breiteneder, H – volume: 10 start-page: e0128402 year: 2015 article-title: Allergen microarray indicates pooideae sensitization in Brazilian publication-title: PLoS One contributor: fullname: Moreira, PFS; Gangl, K; Vieira, FAM; Ynoue, LH; Linhart, B; Flicker, S – volume: 17 start-page: 6 year: 2019 article-title: IgE sensitization in a cohort of adolescents in southern Sweden and its relation to allergic symptoms publication-title: Clin Mol Allergy contributor: fullname: Sterner, T; Uldahl, A; Svensson, A; Borres, MP; Sjölander, S; Tunsäter, A – volume: 189 start-page: 19 year: 2017 end-page: 26 article-title: Recombinant allergy vaccines based on allergen-derived B cell epitopes publication-title: Immunol Lett contributor: fullname: Valenta, R; Campana, R; Niederberger, V – volume: 72 start-page: 4 year: 2006 article-title: Pollen allergic disease: pollens and its major allergens publication-title: Braz J Otorhinolaryngol contributor: fullname: Taketomi, EA; Sopelete, MC; Moreira, PFS; Vieira, FAM – volume: 50 year: 2019 article-title: Two years of treatment with the recombinant grass pollen allergy vaccine BM32 induces a continuously increasing allergen-specific IgG4 response publication-title: EBioMedicine contributor: fullname: Eckl-Dorna, J; Weber, M; Stanek, V; Linhart, B; Ristl, R; Waltl, EE – volume: 5 start-page: 729 year: 2005 end-page: 736 article-title: Identification of grass pollen allergens by two-dimensional gel electrophoresis and serological screening publication-title: Proteomics contributor: fullname: Corti, V; Cattaneo, A; Bachi, A; Rossi, RE; Monasterolo, G; Paolucci, C – volume: 37 start-page: 30 year: 2019 end-page: 35 article-title: Beta-expansin of Bermuda, Johnson, and Para grass pollens, is a major cross-reactive allergen for Allergic Rhinitis patients in subtropical climate publication-title: Asian Pac J Allergy Immunol contributor: fullname: Pacharn, P; Songnual, W; Siriwatanakul, U; Thongngarm, T; Reamtong, O; Jirapongsananuruk, O |
| SSID | ssj0025041 |
| Score | 2.4046526 |
| Snippet | Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim... Lolium multiflorum grass is the major pollen allergen source in the southern region of Brazil, but most of its allergens remain poorly characterized. The aim... |
| SourceID | doaj scielo pubmedcentral proquest gale crossref |
| SourceType | Open Website Open Access Repository Aggregation Database |
| StartPage | e12957 |
| SubjectTerms | Allergen Antigen-antibody reactions Beta-expansin Binding proteins BIOLOGY Care and treatment Grasses Hay-fever Health aspects IgE IgG4 Immunological research Immunotherapy Ion-exchange chromatography Lolium multiflorum Materia medica, Vegetable MEDICINE, RESEARCH & EXPERIMENTAL Methods Plant extracts Pollen |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LixQxEA6yLOJFfOLoukQQVLDZfiWTHHeXXRZxvKgwt5AnNozpZR6y41_xz1rVySzT7MGLh7lMmqZIVaq-Sld9RchbYZ2BvMIUwoYGPzPyQvPAC-Fq5Idy3DLsHZ594Vff209zNt8b9YU1YYkeOG3ciTQBELaXtWsgc5FGCN1OrZesFhUTPEGjiu2SqZxqsbKtMscQk-VJ1VZtAaHyBoeFewhxGI324tBA13_XKd8tlDzEuLTYD0GXj8jDjB3paZL5Mbnn4xNyf5a_jj8lf1LXbcjXcLQP1Oql6W-2OCd6u6Dvz2cfMBXGeEUHhoYurqgDI_zlHcVOE_q5X3Sbn3SoMwwLZGqg13i3ECl4ceyoojo6-K0707stBchp0_wJ2kV6ttS_O7w3oTihZQlOlWbe1tUz8vXy4tv5VZGHLxSWMbYutHFl6ZwGRCI1q_200b61nsvGWlvLSjfGANSs-TQ0NU6_0lw4iIZSNz6I5jk5iH30LwgFTRhhZOUw9eLamLoOwkwrW2lwtNZNyMedItR1YthQmJmA3hTqTY31NiFnqKzbR5Eee_gDjEZlo1H_MpoJeYeqVniIce907kUAiZEOS52C14LEC9DMhByNnoTDZ0fLb3bGonAJK9ai7zcrBZmobAFvocRiZEUj0ccrsfsxMHzj8GVAXiXImQxOZc-ywmq8slTw8jnuCUL0odXq5f_YllfkAb403TAdkYP1cuNfA-Zam-PheP0F21UpbA priority: 102 providerName: Directory of Open Access Journals – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1ba9RAFB5qFfFF6g23VhlBUMFoMslMZh6ktMVSxPVFC_s2zC0aiEmb3ZVd_0r_bM9JstVQBR_ykknCkHOfmfN9hLyQzluoK2wkXZHiNqOIjChEJD1DfCgvHMfe4elncXKafZzx2RbZsKIOP3D-19IO-aRO2-rt6ny9Dwb_vmPvUfG7JEuyCALhDKnAAwQwnt8gN1mWZqjy0-xqWwHhupIBeugfL47CU4fif91XXz8_eQvDVfVnZDreIXeHlJIe9Dpwj2yF-j65PR02zR-Qi74ZtxhW52hTUGda26zWSB-9ruiro-lrrJAxjNEOuKGs59SDbv4MnmIDCv3UVOXyB-2OHxYVAjjQM1xyqCk4d2y0oqb2cC1K2_g1hUzU9bQUtKzpYWt-lbicQpG4pQVfSwc41_lD8uX4w9ejk2jgZIgc53wRGevj2HsDiYoynIU8NSFzQajUOcdUYlJrIQNlIi9ShqRYRkgPQVKZNBQyfUS266YOjwkFSVhpVeKxIhPGWsYKafPEJQb8r_MT8mYjCH3WA29oLFhAbhrlpkFuq99ym5BDFNbVo4ia3d1o2m96MEKtbAHVWlDMp1AFKyulyXIXFGcy4VKYCXmJotaobfjvzNCiADNGlCx9AM4M6jFIciZkb_Qk2KQbDT_fKIvGITzIVodmOddQoKoM0jCcsRxp0Wjq45G6_N4BfyMnMyRkMcyzVzi9sRc8pBfHGj7e6TJm7l0H1u5_TOQJuYPv9OtKe2R70S7DU8i0FvZZZz2XxwQnKQ priority: 102 providerName: Scholars Portal |
| Title | Identification of carboxymethyl (CM)-binding proteins derived from Lolium multiflorum pollen extract and antibody reactivity in Brazilian allergic patients |
| URI | https://search.proquest.com/docview/2879408157 https://pubmed.ncbi.nlm.nih.gov/PMC10578120 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100670&lng=en&tlng=en https://doaj.org/article/9bf666e92d34469b88a47ce95281586a |
| Volume | 56 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3di9NAEF_uDhFfxE-snscKggrmms_N7uNd8TjEiKBC35b9yhlIk5K2cvVf8Z91JtnohXvzoXnohnbIzM7MbzPzG0Jec2M14AodcFMm-JqRBYqVLOA2Rn4oy0yGvcPFZ3b5Pf24zJYHhI29MH3RvtHVaVOvTpvqR19buV6Z-VgnNv9SLHA2LQSmcH5IDvMkGTG6h1lZmA5TCMMQ9rpYeq6hTITzKI3SAELmEoeGOwh1GJVuxKOetv-2c75dMHkH41N9MxRdPCD3fQ5JzwZZH5ID1zwidwv_lvwx-T1035b-OI62JTWq0-31HudF72v6dlG8Q0iMcYv2TA1Vs6EWjPGnsxQ7Tuintq52K9rXG5Y1MjbQNZ4xNBS8OXZWUdVY-Gwr3do9hdTTDHMoaNXQ8079qvD8hOKklg6cK_X8rZsn5OvFh2-Ly8APYQhMlmXbQGkbhtYqyEyEymKXJ8qlxjGRGGNiEalEa0g5Y5aXSYxTsBTjFqKiUIkrefKUHDVt454RClrRXIvIIgRjSus4LrnOIxMpcLjGzsj7URFyPTBtSEQooDeJepOgt-t_epuRc1TW31uRJrv_ou2upDcWKXQJ8MyJ2CYAe4XmXKW5cSKLeZRxpmbkDapa4mbGZ6d8TwJIjLRY8gy8FwAwyGpm5HhyJ2xCM1l-NRqLxCWsXGtcu9tIQKQihbwLJeYTK5qIPl0Bw--ZvkdDBzkHg5Pew2ywKi8MJdo3PhNM1fuWq-f__ycvyD38qeF86ZgcbbudewkZ11af9CcVcC1SftJvtj8UpCzz |
| link.rule.ids | 230,315,733,786,790,870,891,2115,24346,27955,27956,53825,53827 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LbxMxELZKQZQLb0SggJGQAIlN9mXHPrYRVYCkQqKI3Cy_lq5IdqNkg2j_Cn-WmX1AQ09wyCVeJd7155n5vDPfEPJCWGeAV5hA2CzB14w80DzjgXAx6kM5bhnWDk-P-fhz-n7GZjuEd7UwddK-NXm_mC_6RX5a51YuF3bQ5YkNPk5H2JsWHFM4uEKuwoaNWcfSW6LFwrTpQxiGsNvlrFUbYjIcRGmUBuA0Z9g23IOzQ790wSPVwv2XzfPllMlr6KHmF53R0S3ypbuNJgflW39Tmb49_0vh8d_v8za52can9KAZv0N2fHGXXJ-2b-DvkZ9NZW_WHvXRMqNWrwzMA3tRn83pq9H0NdJt9Im0VoHIizV1APTv3lGsZqGTcp5vFrTOZczmqAZBl3h-UVDwFFi1RXXh4FPlpnRnFMJa2_S4oHlBD1f6PMezGYpdYFZguGmrDbu-Tz4dvT0ZjYO2wUNgGWNVoI0LQ-c0RD1Ss9gPE-1T67lMrLWxjHRiDISzMR9mSYwdtjQXDjyu1InPRPKA7BZl4R8SCutthJGRQ3rHtTFxnAkzjGykwZhb1yNvuiVWy0bFQyH7AUQoRIQCRPz4g4geOUQY_L4UJbjrL8rVV9UukJImA-rnZewSoNTSCKHTofWSxSJiguseeYkgUmgo8Nnptt4BZoySW-oALCOQO4iYemR_60rY4HZr-HkHQ4VDmBVX-HKzVsB2ZQoxHc5YbOFza-rbIwC2WkW8AxfMs4Gyaq3XGjP-wlDhzsFngjSgLud69P9_8ozsjU-mEzV5d_zhMbmBP9ucY-2T3Wq18U8gsqvM03ob_wKe2U1D |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF5BQRUX3ohAgUVCAiSc-J3dYxuICjRVJUCKxGG1L4OFs46cBNH-Ff4sM_YaGnrrIZd4lay9387Mt575hpAXTBsFvEIFTBcJvmbMA5kXecBMjPpQJtcZ1g7PjvPDL-mHeTb3WZUrn1bptCqHrloMXfm9za1cLvSozxMbncwm2JsWHFM4WppidJVcg00bj3um7slWFqZdL8IwhB3P515xKOPhKEqjNADHOcfW4RYcHvqmc16pFe-_aKIvpk1eRy9VnXdI01vka38rXR7Kj-FmrYb67D-Vx8vd621y08epdL8bc4dcse4u2Z35N_H3yO-uwrfwR360LqiWjYK5YE_q04q-msxeI-1G30hbNYjSragBwP-0hmJVCz2qq3KzoG1OY1GhKgRd4jmGo-AxsHqLSmfgsy5VbU4phLe663VBS0cPGnlW4hkNxW4wDRhw6jViV_fJp-m7z5PDwDd6CHSWZetAKhOGxkiIfrjMYjtOpE21zXmitY55JBOlIKyN83GRxNhpS-bMgOflMrEFSx6QHVc7-5BQWHPFFI8M0rxcKhXHBVPjSEcSjLo2A_KmX2ax7NQ8BLIgQIVAVAhAxa9_qBiQA4TC36Eoxd1-UTffhF8kwVUBFNDy2CRArbliTKZjbXkWsyhjuRyQlwgkgQYDn530dQ8wY5TeEvtgIYHkQeQ0IHtbI2Gj663Lz3soCryE2XHO1puVANbLU4jtcMZsC6NbU9--AoBr1cR7gME8OzgLb8VWmPkXhgJ3Dz4TpANtWdejy__JM7J78nYqjt4ff3xMbuCvdsdZe2Rn3WzsEwjw1uppu5P_APTAT8M |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Identification+of+carboxymethyl+%28CM%29-binding+proteins+derived+from+Lolium+multiflorum+pollen+extract+and+antibody+reactivity+in+Brazilian+allergic+patients&rft.jtitle=Brazilian+journal+of+medical+and+biological+research&rft.au=Correa%2C+A+S&rft.au=Miranda%2C+J+S&rft.au=Oliveira%2C+L+A+R&rft.au=Moreira%2C+P+F+S&rft.date=2023-01-01&rft.eissn=1414-431X&rft.volume=56&rft.spage=e12957&rft.epage=e12957&rft_id=info:doi/10.1590%2F1414-431X2023e12957&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0100-879X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0100-879X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0100-879X&client=summon |