Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 107; no. 17; pp. 7811 - 7816
• Main Authors: Lynch-Day, Molly A, Bhandari, Deepali, Menon, Shekar, Huang, Ju, Cai, Huaqing, Bartholomew, Clinton R, Brumell, John H, Ferro-Novick, Susan, Klionsky, Daniel J
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 27.04.2010; National Acad Sciences
• Subjects: Antibodies; Autophagy; Autophagy - physiology; Biological Sciences; Biological transport; Chromatography, Gel; Cytoplasm; DNA Primers - genetics; Eukaryotes; Fluorescence; Guanine Nucleotide Exchange Factors - metabolism; Immunoblotting; Immunoprecipitation; Lipids; Membranes; Microscopy, Fluorescence; P branes; Phagosomes - metabolism; rab GTP-Binding Proteins - metabolism; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins - metabolism; Secretory pathway; Starvation; Traffic; Vacuoles; Vesicular Transport Proteins - metabolism; Yeasts

Macroautophagy (hereafter autophagy) is a ubiquitous process in eukaryotic cells that is integrally involved in various aspects of cellular and organismal physiology. The morphological hallmark of autophagy is the formation of double-membrane cytosolic vesicles, autophagosomes, which sequester cytop...