Amino acid 159 of the envelope protein affects viral replication and T-cell infiltration by West Nile virus in intracranial infection

• Published in: Scientific reports Vol. 10; no. 1; p. 7168
• Main Authors: Kobayashi, Shintaro, Kaneko, Chisato, Kawakami, Ryoko, Hasebe, Rie, Sawa, Hirofumi, Yoshii, Kentaro, Kariwa, Hiroaki
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.04.2020; Nature Publishing Group
• Subjects: 13/1; 13/109; 13/51; 38/109; 38/77; 631/326/596/1879; 631/326/596/2555; 64/60; Amino acids; Animals; Brain - metabolism; Brain - pathology; Brain - virology; CD3 antigen; CD8 antigen; Cell culture; Chlorocebus aethiops; Encephalitis; HEK293 Cells; Humanities and Social Sciences; Humans; Infiltration; Lymphocytes T; Mice; Mortality; multidisciplinary; Neurovirulence; Pathogenicity; Pathogens; Proteins; Replication; Science; Science (multidisciplinary); Structural proteins; T-Lymphocytes - metabolism; T-Lymphocytes - pathology; Vector-borne diseases; Vero Cells; Viral envelope proteins; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Virus Replication; West Nile Fever - genetics; West Nile Fever - metabolism; West Nile Fever - pathology; West Nile virus; West Nile virus - physiology

West Nile virus (WNV) is an important cause of viral encephalitis in birds and animals, including humans. Amino acid 159 of the envelope (E) protein is reportedly implicated in the different levels of neurovirulence in mice infected with WNV NY99 or Eg101. We investigated the role of amino acid 159...